[1]高谋,徐如祥,杨志军,等.两种干细胞对颅脑创伤炎症反应调控作用的对比研究[J].第三军医大学学报,2015,37(17):1697-1703.
 Gao Mou,Xu Ruxiang,Yang Zhijun,et al.Comparison on immunomodulatory effects of 2 kinds of transplanted stem cells on inflammation secondary to traumatic brain injury in mice[J].J Third Mil Med Univ,2015,37(17):1697-1703.
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两种干细胞对颅脑创伤炎症反应调控作用的对比研究(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
37卷
期数:
2015年第17期
页码:
1697-1703
栏目:
论著
出版日期:
2015-09-15

文章信息/Info

Title:
Comparison on immunomodulatory effects of 2 kinds of transplanted stem cells on inflammation secondary to traumatic brain injury in mice
作者:
高谋徐如祥杨志军董勤姚慧张洪钿杨阳刘宁陈晨朱建伟邹明明籍新潮
第三军医大学研究生管理大队;北京军区总医院附属八一脑科医院神经外科;首都医科大学附属复兴医院神经内科
Author(s):
Gao Mou Xu Ruxiang Yang Zhijun Dong Qin Yao Hui Zhang Hongtian Yang Yang Liu Ning Chen Chen Zhu Jianwei Zou Mingming Ji Xinchao

Postgraduate Team, Third Military Medical University, Chongqing, 400038; Department of Neurosurgery,  Bayi Brain Hospital, General Hospital of Beijing Military Command, Beijing, 100700; Department of Neurology, Fuxing Hospital, Capital Medical University, Beijing, 100038, China

关键词:
神经干细胞间充质干细胞颅脑创伤炎症反应细胞因子
Keywords:
neural stem cells mesenchymal stem cells traumatic brain injury inflammation cytokine
分类号:
R318.2; R392.3; R651.1
文献标志码:
A
摘要:

目的      比较神经干细胞(neural stem cells,NSCs)与间充质干细胞(mesenchymal stem cells, MSCs)移植对颅脑创伤(traumatic brain injury,TBI)后炎症反应的调控作用。      方法      将264只成年雄性C57BL/6小鼠按随机数字表法分为假手术组(54只)和TBI组(162只), 采用自由落体脑打击装置制备TBI模型并于伤后12 h进行NSS评分,将3~7分者按随机数字表法分为3组,每组54只,于伤后24 h将离体培养的同品系小鼠成体NSCs、MSCs和PBS分别定向注射到创伤模型鼠脑内。于移植后24、72 h和7 d处死动物,取脑组织病理切片染色,观察脑损伤修复和免疫细胞浸润情况;分别提取脑组织mRNA和蛋白检测细胞因子表达量。      结果      ①NSCs或MSCs移植7 d后, 脑损伤较PBS处理组局限,组织内渗出减少。②NSCs或MSCs移植72 h和7 d后,脑组织内浸润的小胶质细胞/巨噬细胞数量明显少于PBS处理组(P<0.05);NSCs移植7 d后,脑内浸润的T细胞数量明显少于PBS处理组(P<0.05)。③MSCs移植后IL-6和TNF-α mRNA和蛋白水平在各时间点均低于PBS处理组(P<0.05);NSCs移植后IL-10 mRNA水平在24 h和72 h明显高于PBS处理组(P<0.05),其蛋白水平在72 h和7 d也较PBS处理组和MSCs移植组明显升高(P<0.05)。      结论       NSCs与MSCs可影响TBI后炎症反应中免疫细胞浸润和细胞因子表达,二者调控作用既有相同之处,也有明显差异。

Abstract:

Objective      To compare the immunomodulatory effects of transplanted neural stem cells (NSCs) and mesenchymal stem cells (MSCs) on inflammation secondary to traumatic brain injury (TBI).       Methods      A total of 264 adult male C57BL/6 mice were randomly assigned into sham operation group (n=54) and TBI group (n=162). Animal models of TBI were established using a free-falling weight drop device, and after 12 h, the mice with neurological severity score scores of 3-7 points were selected for further research. The syngeneic adult NSCs, MSCs and PBS were injected into the brain of TBI mice with aid of a stereotaxic apparatus at 24 h after injury. Then, the mice were randomly sacrificed at 24 and 72 h and 7 d after injection, and the brain sections were stained for morphological analysis and evaluation of infiltrated immune cells. Besides, the expression of cytokines in the brain tissues were detected.       Results      In contrast to the PBS-treated group, the brain damage and tissue exudation were reduced obviously in the NSCs- or MSCs-transplanted group after 7 d. The number of microglias/macrophages in the NSCs- or MSCs-transplanted group was significantly less than that in the PBS-treated group at 72 h and on 7 d(P<0.05), and the number of T cells in the NSCs-transplanted group was significantly less than that in the PBS-treated group on the 7th day (P<0.05). The mRNA and protein levels of interleukin (IL)-6 and tumor necrosis factor (TNF)-α in the MSCs-transplanted group were significantly lower than those in the PBS-treated group (P<0.05). The IL-10 mRNA levels in the NSCs-transplanted group were significantly higher than those in the PBS-treated group at 24 and 72 h (P<0.05), and the IL-10 protein levels were also significantly higher than those in the PBS-treated group and the MSCs-transplanted group at 72 h and on 7 d (P<0.05).       Conclusion      Transplanted NSCs and MSCs can affect the infiltration of immune cells and the expression of cytokines so as to regulate the inflammation after TBI.

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更新日期/Last Update: 2015-09-07