[1]魏蕾,江黎珠,于超,等.下调FoxM1表达对人喉癌Hep-2细胞顺铂敏感性的影响[J].第三军医大学学报,2015,37(16):1603-1608.
 Wei Lei,Jiang Lizhu,Yu Chao,et al.FoxM1 down-regulation promotes sensitivity of laryngeal carcinoma Hep-2 cells to cisplatin[J].J Third Mil Med Univ,2015,37(16):1603-1608.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
37卷
期数:
2015年第16期
页码:
1603-1608
栏目:
论著
出版日期:
2015-08-30

文章信息/Info

Title:
FoxM1 down-regulation promotes sensitivity of laryngeal carcinoma Hep-2 cells to cisplatin
作者:
魏蕾江黎珠于超文韬宇陈鸿雁
重庆医科大学附属第一医院耳鼻喉科
Author(s):
Wei Lei Jiang Lizhu Yu Chao Wen Taoyu Chen Hongyan

Department of Otolaryngology, the First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China

关键词:
FoxM1硫链丝菌肽Hep-2细胞顺铂化疗敏感性细胞凋亡
Keywords:
forkhead box protein M1 thiostrepton Hep-2 cells cisplatin chemosensitivity apoptosis
分类号:
R73-361;R739.65;R979.19
文献标志码:
A
摘要:

目的      探讨下调FoxM1表达对人喉癌Hep-2细胞顺铂敏感性的影响及其可能机制。      方法      分别选用FoxM1 siRNA以及FoxM1特异性抑制剂(硫链丝菌肽)下调Hep-2细胞FoxM1表达。实时定量PCR、Western blot检测siRNA或硫链丝菌肽处理细胞后FoxM1 mRNA、蛋白表达量;MTT法检测下调FoxM1表达后Hep-2细胞的顺铂敏感性;流式细胞术检测下调FoxM1表达后顺铂诱导的凋亡率变化;实时定量PCR、Western blot检测顺铂作用于FoxM1下调组及对照组细胞,其FoxM1 mRNA、蛋白及凋亡相关蛋白Bcl-2、Bax表达变化。      结果      siRNA及硫链丝菌肽均能下调FoxM1表达(P<0.05);两种方式均能降低顺铂作用下细胞存活率以及IC50[NC组顺铂IC50=(2.609±0.102)μg/mL,干扰组IC50=(0.771±0.058)μg/mL,P<0.05;对照组顺铂IC50=(2.142±0.198)μg/mL,抑制剂组IC50=(0.773±0.063)μg/mL,P<0.05],siRNA法处理后NC组、干扰组、NC+顺铂组、干扰+顺铂组凋亡率依次为(4.197±0.273)%、(12.553±0.183)%、(37.465±4.305)%、(82.373±7.214)%,干扰+顺铂组凋亡率显著升高(P<0.05);抑制剂处理后对照组、抑制剂组、顺铂组、抑制剂+顺铂组凋亡率依次为(2.343±0.194)%、(10.127±0.479)%、(35.075±1.995)%、(62.843±1.824)%,抑制剂+顺铂组凋亡率显著升高(P<0.05);下调FoxM1表达,凋亡抑制蛋白Bcl-2下调,促凋亡蛋白Bax表达上调(P<0.05)。      结论      下调FoxM1表达可提高Hep-2细胞对顺铂的敏感性,其机制可能与凋亡相关蛋白Bcl-2下调、Bax上调相关。

Abstract:

Objective      To determine the effect of down-regulation of forkhead box protein M1 (FoxM1) on the cisplatin sensitivity of human laryngeal carcinoma Hep-2 cells and investigate the potential mechanisms.       Methods      Small interfering RNA (siRNA) and FoxM1 inhibitor thiostrepton were used respectively to suppress FoxM1 expression in Hep-2 cells. The expression of FoxM1 at mRNA and protein levels was detected by qRT-PCR and Western blotting respectively. Cell viability and apoptosis of Hep-2 cells were detected by MTT assay and flow cytometry. Furthermore, qRT-PCR and Western blotting were used to investigate the changes of FoxM1 in Hep-2 cells with FoxM1 suppression and cisplatin treatment. The changes of apoptosis-related proteins were detected by Western blotting.       Results      Both of siRNA and thiostrepton suppressed FoxM1 expression, reduced the cell survival after cisplatin treatment and IC50 (cisplatin treated normal cells IC50=2.609±0.102  g/mL, siRNA infected cells IC50=0.771±0.058  g/mL, P<0.05; cisplatin treated cells IC50=2.142±0.198  g/mL, thiostrepton treated cells IC50=0.773±0.063  g/mL, P<0.05). The apoptotic rate was (4.197±0.273)%, (12.553±0.183)%, (37.465±4.305)% and (82.373±7.214) % respectively in the normal Hep-2 cells and the cells with siRNA infection, cisplatin treatment and infection plus cisplatin treatment, with that of the latter group of cells the highest (P<0.05). The rate was (2.343 3±0.193 6)%, (10.127±0.479)%, (35.075±1.995)% and (62.843±1.824)% separately in the normal Hep-2 cells and the cells after thiostrepton treatment, cisplatin treatment and thiostrepton plus cisplatin treatment, with that of the latter group of cells the highest (P<0.05). Down-regulation of FoxM1 inhibited the expression of Bcl-2 and up-regulated that of Bax.       Conclusion      FoxM1 promotes the sensitivity of Hep-2 cells to cisplatin, which potentially is due to its down-regulation of Bcl-2 and up-regulation of Bax.

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相似文献/References:

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 Lin Li,Deng Bi,Shou Zhu,et al.Effect of thiostrepton on proliferation and FoxM1 expression in human laryngocarcinoma Hep-2 cell line[J].J Third Mil Med Univ,2012,34(16):2086.
[2]于超,李艮平,陈鸿雁.硫链丝菌肽抑制FOXM1表达后对裸鼠鼻咽癌移植瘤的抑制作用[J].第三军医大学学报,2015,37(20):2038.
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更新日期/Last Update: 2015-08-19