[1]王钰,龙爽,谭力,等.粒细胞集落刺激因子激活β-catenin促进创面修复[J].陆军军医大学学报(原第三军医大学学报),2015,37(16):1648-1651.
 Wang Yu,Long Shuang,Tan Li,et al.Granulocyte colony-stimulating factors promote wound healing by activation of β-catenin in green fluorescent protein chimeric mice[J].J Amry Med Univ (J Third Mil Med Univ),2015,37(16):1648-1651.
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陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
37卷
期数:
2015年第16期
页码:
1648-1651
栏目:
论著
出版日期:
2015-08-30

文章信息/Info

Title:
Granulocyte colony-stimulating factors promote wound healing by activation of β-catenin in green fluorescent protein chimeric mice
作者:
王钰龙爽谭力陈泽林史春梦粟永萍
第三军医大学军事预防医学院全军复合伤研究所,创伤、烧伤与复合伤国家重点实验室
Author(s):
Wang Yu Long Shuang Tan Li Chen Zelin Shi Chunmeng Su Yongping

State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury, College of Military Preventive Medicine, Third Military Medical University, Chongqing, 400038, China

关键词:
粒细胞集落刺激因子创面愈合&beta-连环蛋白
Keywords:
granulocyte colony-stimulating factor wound healing &beta-catenin
分类号:
R331.22;R64;R977.1
文献标志码:
A
摘要:

目的      利用转绿色荧光蛋白(green fluorescent protein, GFP)嵌合小鼠的动物模型,研究粒细胞集落刺激因子(granulocyte colony-stimulating factor,G-CSF)骨髓动员在创面修复中的作用和机制。      方法      C57骨髓嵌合小鼠随机分成骨髓动员组和对照组。骨髓动员组小鼠连续5 d予以皮下注射G-CSF,对照组小鼠给予相同剂量的生理盐水。在小鼠背部制作皮肤全层缺损伤,1.3 cm×1.3 cm大小。骨髓动员组继续给予G-CSF 3 d,对照组给予生理盐水。伤后每天观察各组动物创面大小及愈合情况。取创伤后第1天的创面组织,消化成单细胞悬液,流式检测GFP+细胞比例。取伤后第3天对照组和骨髓动员组创面组织制取石蜡切片,免疫组化检测β-catenin 表达。      结果      骨髓动员组小鼠创面愈合增快为(12.5±0.9) d,对照组愈合时间为(18.3±0.8) d。骨髓动员组在伤后1 d创面局部GFP+细胞明显多于对照组,差异有统计学意义(P<0.05)。骨髓动员组创面处β-catenin在创缘组织中的表达明显增强,与对照组相比差异具有统计学意义(P<0.05),并部分呈现核表达。      结论      G-CSF动员后,GFP+细胞大量聚集于创面,并促进创面β-catenin的表达和核转位,加快创面愈合。

Abstract:

Objective      To determine the effect of granulocyte colony-stimulating factor (G-CSF) stimulation on cutaneous wound healing in green fluorescent protein (GFP) chimeric mice and investigate the underlying mechanism.       Methods      C57BL/6 mice were exposed to 8.5 Gy 60Co γ irradiation and then received 5×106 bone marrow cells derived from C57BL/6-EGFP mice through tail vein in 4 h later to establish GFP chimeric mice. The spleen of the chimeric mice was resected in 2 months after irradiation, and then divided into 2 groups, bone marrow mobilization group (G-CSF group) and control group. G-CSF of 50 μg·(kg·d)-1  was injected to the mice of the G-CSF group for 5 d before and for 3 d after wound cutting (1.3 cm×1.3 cm) on their back. Normal saline instead of G-CSF was injected in the mice of control group after wounding. Residual wound area and wound healing time were observed. On day 1 after wounding, the wound tissues were harvested and digested into single cell suspension to detect the ratio of GFP+ cells by flow cytometry. On day 3, wound tissues were collected again for histological analysis of β-catenin expression.       Results      G-CSF treatment accelerated wound closure in mice than the control mice (12.5±0.9 vs 18.3±0.8 d). There were more GFP+ cells on wound area in the mobilized group than the control mice (P<0.05) on day 1. The expression of β-catenin was significantly enhanced in the wound of the mobilized group and mainly in located in the nuclei (P<0.05).       Conclusion      G-CSF mobilization aggregates GFP+ cells in the wound area, promotes the expression and nuclear translocation of β-catenin, and accelerates wound healing after skin injury.

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更新日期/Last Update: 2015-08-19