[1]赵欣,张健.RGD修饰共载紫杉醇与microRNA-34a脂质体对肺癌裸鼠移植瘤的生长抑制作用[J].陆军军医大学学报(原第三军医大学学报),2015,37(12):1243-1247.
 Zhao Xin,Zhang Jian.Effect of RGD-modified paclitaxel and microRNA-34a co-loaded liposome on inhibiting tumor growth in A549-bearing nude mice[J].J Amry Med Univ (J Third Mil Med Univ),2015,37(12):1243-1247.
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RGD修饰共载紫杉醇与microRNA-34a脂质体对肺癌裸鼠移植瘤的生长抑制作用(/HTML )
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陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
37卷
期数:
2015年第12期
页码:
1243-1247
栏目:
论著
出版日期:
2015-06-30

文章信息/Info

Title:
Effect of RGD-modified paclitaxel and microRNA-34a co-loaded liposome on inhibiting tumor growth in A549-bearing nude mice
作者:
赵欣张健
新乡医学院第一附属医院:呼吸科,儿童重症监护室
Author(s):
Zhao Xin Zhang Jian

Department of Respiratory Diseases, Pediatric Intensive Care Unit, First Affiliated Hospital, Xinxiang Medical University, Xinxiang, Henan Province, 453000, China

关键词:
整合素受体紫杉醇microRNA-34a肺癌
Keywords:
integrin receptor paclitaxel microRNA-34a lung cancer
分类号:
R734.2;R969;R979.1
文献标志码:
A
摘要:

目的      探讨精氨酸-甘氨酸-天冬氨酸(Arg-Gly-Asp,RGD)修饰共载紫杉醇与microRNA-34a脂质体(RGD miLPs-34a/PTX)的体内药代动力学以及体内对肺癌裸鼠移植瘤的生长抑制作用。      方法      采用荧光分光光度法检测RGD miLPs-34a/PTX的体外血清稳定性和体内药代动力学。构建肺癌裸鼠移植瘤模型,近红外活体成像研究RGD miLPs-34a/PTX在荷瘤裸鼠的体内分布。荷瘤裸鼠治疗实验检测RGD miLPs-34a/PTX对肿瘤的生长抑制作用。      结果      RGD miLPs-34a/PTX在50%血清中具有良好的稳定性。药代动力学实验表明,在荷瘤小鼠血液中的microRNA-34a的消除速率明显快于miLPs-34a/PTX和RGD miLPs-34a/PTX组,差异有统计学意义(P<0.05);活体成像实验结果显示,RGD miLPs-34a/PTX在肿瘤组织的荧光分布显著强于miLPs-34a/PTX。肿瘤生长抑制实验结果显示,RGD miLPs-34a/PTX对肿瘤生长的抑制作用显著强于其他脂质体组,差异有统计学意义(P<0.01)。各给药组对肿瘤生长抑制能力显著强于生理盐水组,差异有统计学意义(P<0.01)。      结论      体内研究证实RGD修饰的共载紫杉醇和miRNA脂质体能够延长药物体内循环时间,达到长循环效果,具有良好的体内肿瘤靶向性和体内肿瘤治疗效果。

Abstract:

Objective      To research the pharmacokinetics and antitumor effect of arginine-glycine-aspartic acid (RGD)-modified paclitaxel and microRNA-34a co-loaded liposome (RGD miLPs-34a/PTX) in A549-bearing nude mice.        Methods      Fluorescence spectrophotometry was used to evaluate in vitro stability of RGD miLPs-34a/PTX in 50% fetal bovine serum (FBS) and in vivo pharmacokinetics. Near infrared imaging was adopted to evaluate the targeting efficiency of the liposome. A549 cells were xenografted into athymic mice to establish lung cancer mouse models, which were used to evaluate the antitumor effect of the liposome.       Results      RGD miLPs-34a/PTX could be stable in 50% FBS for 24 h. The elimination of microRNA-34a was much quicker than that of miLPs-34a/PTX and RGD miLPs-34a/PTX (P<0.05). Near infrared imaging demonstrated that the accumulation of RGD miLPs-34a/PTX was much higher than that of miLPs-34a/PTX. The antitumor effect of RGD miLPs-34a/PTX was much better than that of other liposomes (P<0.05).        Conclusion      RGD miLPs-34a/PTX shows high targeting efficiency and antitumor effect, and is a promising drug delivery system targeting lung cancer.

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更新日期/Last Update: 2015-06-24