[1]易茜,蹇朝,李勇,等.氧化应激标志物在房颤患者心房结构重构中的变化及意义[J].陆军军医大学学报(原第三军医大学学报),2015,37(01):55-59.
 Yi Qian,Jian Zhao,Li Yong,et al.Changes and significance of oxidative stress biomarkers in atrial structural remodeling in atrial fibrillation patients[J].J Amry Med Univ (J Third Mil Med Univ),2015,37(01):55-59.
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陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
37卷
期数:
2015年第01期
页码:
55-59
栏目:
论著
出版日期:
2015-01-15

文章信息/Info

Title:
Changes and significance of oxidative stress biomarkers in atrial structural remodeling in atrial fibrillation patients
作者:
易茜蹇朝李勇肖颖彬陈林唐富琴马瑞彦
第三军医大学新桥医院全军心血管外科研究中心
Author(s):
Yi Qian Jian Zhao Li Yong Xiao Yingbin Chen Lin Tang Fuqin Ma Ruiyan

Center of Cardiovascular Surgery, Xinqiao Hospital, Third Military Medical University, Chongqing, 400037, China

关键词:
房颤心房重构氧化应激NADPH氧化酶晚期氧化蛋白产物
Keywords:
atrial fibrillation atrial remodeling oxidative stress nicotinamide adenine dinucleotide phosphate oxidase advanced oxidative protein products
分类号:
R362; R364.33; R541.75
文献标志码:
A
摘要:

目的      通过分析多种氧化应激标志物在血清及组织中的动态变化,观察其在心房结构重构中的表达,探讨房颤(atrial fibrillation,AF)的发生机制。      方法        选择我中心44例2014年2-5月行单纯二尖瓣置换手术的风湿性心脏病患者,根据病史及术前动态心电图记录分为2组:持续性房颤(AF)组26例,窦性心律(SR)组18例。术中取部分右心耳组织,行Masson染色观察纤维化情况,采用免疫组化、免疫荧光染色法观察右心耳组织NOX2蛋白表达,用ELISA法测定右心耳组织中丙二醛(malondialdehyde,MDA)、超氧化物歧化酶(superoxide dismutase,SOD)、活性氧(reactive oxygen species,ROS)、晚期氧化蛋白产物(advanced oxidative protein products,AOPP)浓度以及血清中ROS、AOPP含量。       结果         AF组心房组织中MDA[(15.41±1.69)μmol/mg]、SOD[(13.32±1.25)μmol/mg]、ROS[(18.53±3.47)μmol/mg]、AOPP[(29.69±2.09)μmol/mg]含量与SR组MDA[(11.46±1.83)μmol/mg]、SOD[(10.65±1.15)μmol/mg]、ROS[(13.65±3.29)μmol/mg]、AOPP[(21.94±1.94)μmol/mg]比较差异有统计学意义(P<0.05),AF组血清中ROS含量[(34.6±2.61)μmol/L]明显高于SR组[(17.8±1.03)μmol/L],2组患者血清中AOPP含量差异无统计学意义 (P>0.05)。与SR组相比,AF组右心耳组织胶原纤维增生明显,NOX2蛋白表达呈强阳性。      结论        房颤时发生以间质纤维化改变的心房结构重构,氧化应激参与了房颤发生和结构重构过程。

Abstract:

Objective       To detect the levels and the dynamic expression levels of several oxidative stress biomarkers in the serum and cardiac tissues during atrial remodeling in atrial fibrillation patients in order to investigate the underlying mechanism of atrial fibrillation development.       Methods       A total of 44 patients with rheumatic heart disease undergoing mitral valve replacement in our center from February to May 2014 were enrolled in the present study. The patients were divided into 2 groups according to their history and preoperative electrocardiography (ECG) reports, that is persistent atrial fibrillation group (AF,n=26) and sinus rhythm group (SR, n=18). Right auricle tissue samples were obtained during the operation, and then stained by Masson trichrome staining to examine the degree of fibrosis. The protein expression of NOX2 was measured by immunohistochemical assay and laser confocal microscopy. Oxidative stress was estimated by the amount of malondialdehyde (MDA), superoxide dismutase (SOD), reactive oxygen species (ROS), advanced oxidative protein products (AOPP) in the right auricle, and by the serum levels of ROS and AOPP by enzyme-linked immunosorbent assay (ELISA).       Results       Significant differences were seen in the amount of MDA (15.41±1.69 vs 11.46±1.83  mol/mg), SOD (13.32±1.25 vs 10.65±1.15  mol/mg), ROS (18.53±3.47 vs 13.65±3.29  mol/mg), AOPP (29.69±2.09 vs 21.94±1.94  mol/mg) between the AF group and SR group (P<0.05). The serum ROS level was higher in the AF group than SR group (34.6±2.61 vs 17.8±1.03  mol/L), but there was no obvious difference in that of AOPP (P>0.05). Compared to the SR group, the AF group had markedly more collagenous fibers and stronger NOX2 protein expression in the right auricle.       Conclusion       Myocardial interstitial fibrosis is the main characteristics of atrial structural remodeling in atrial fibrillation, and oxidative stress is involved in the process of atrial fibrillation and structural remodeling.

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[2]曹哲哲,马瑞彦,蹇朝,等.CaMKⅡ和PLB在房颤心房重构中的作用及意义[J].陆军军医大学学报(原第三军医大学学报),2019,41(03):243.
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更新日期/Last Update: 2015-01-05