[1]王本瀚,宋来君,姚安会,等.不同刺激对N9小胶质细胞炎性细胞因子分泌及存活的影响[J].陆军军医大学学报(原第三军医大学学报),2014,36(18):1899-1903.
 Wang Benhan,Song Laijun,Yao Anhui,et al.Inflammatory cytokines expression and survival of N9 microglia after different stimulations[J].J Amry Med Univ (J Third Mil Med Univ),2014,36(18):1899-1903.
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不同刺激对N9小胶质细胞炎性细胞因子分泌及存活的影响(/HTML )
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陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
36卷
期数:
2014年第18期
页码:
1899-1903
栏目:
论著
出版日期:
2014-09-30

文章信息/Info

Title:
Inflammatory cytokines expression and survival of N9 microglia after different stimulations
作者:
王本瀚宋来君姚安会李经纶唐斌刘伟卫润斐曹付强
解放军第153中心医院神经外科;郑州大学第一附属医院神经外科
Author(s):
Wang Benhan Song Laijun Yao Anhui Li Jinglun Tang Bin Liu Wei Wei Runfei Cao Fuqiang

Department of Neurosurgery, No. 153 Central Hospital of PLA, Zhengzhou, Henan Province, 450042; Department of Neurosurgery, First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan Province, 450052, China

关键词:
N9小胶质细胞极化炎性细胞因子细胞死亡
Keywords:
N9 microglia polarization inflammatory cytokines cell death
分类号:
R322.8;R363.27;R392.11
文献标志码:
A
摘要:

目的       探讨不同刺激对N9小胶质细胞炎性细胞因子分泌及存活的影响。       方法       体外培养N9小胶质细胞,分为空白对照组(Control组)、M1型刺激组(LPS+IFN-γ组)、M2型刺激组(IL-4组)。刺激24 h后,通过实时荧光定量PCR及ELISA检测N9小胶质细胞在极化状态下细胞因子的表达;同时体外培养N9小胶质细胞,分为Control组、LPS组、IFN-γ组、LPS+IFN-γ组及IL-4组,通过Hoechst-PI染色的方法检测不同因子对细胞死亡的影响,并用Western blot检测各组Caspase-3的表达。       结果       LPS+IFN-γ组N9细胞促炎因子表达明显高于Control组及IL-4组(P<0.01),IL-4组N9细胞抑炎因子的表达明显高于Control组及LPS+IFN-γ组(P<0.01)。在细胞存活方面,IFN-γ组及LPS+IFN-γ组N9细胞的死亡数及Caspase-3的表达明显高于Control组、LPS组、IL-4组(P<0.05,P<0.01)。       结论       N9细胞可发生极化,在不同极化状态表达不同的炎性因子;IFN-γ可影响N9细胞的存活,其可能是通过Caspase-3而导致细胞死亡。

Abstract:

Objective       To investigate inflammatory cytokines expression and survival of N9 microglia after different stimulations.        Methods       In vitro, cultured N9 microglias were divided into a control group, a LPS+IFN-γ  stimulation group (M1) and an IL-4 stimulation group (M2). Total RNA were extracted and inflammatory cytokine mRNA were detected through Q-RT-PCR and total culture media were collected to detect the concentration of inflammatory cytokines through ELISA, 24 h after stimulation. N9 microglias were divided into a control group, a LPS group, an IFN-γ group, a LPS+IFN-γ group, and an IL-4 group to study the effects of the cytokines on N9 microglia survival. The survival rates of each group were detected through Hoechst-PI staining. The expression of caspase-3 was detected by Western blotting.        Results       In the aspect of inflammatory cytokines secretion, the cells in group M1 expressed more pro-inflammatory cytokines than those in the control group and group M2 (P<0.01). The cells in group M2 expressed more anti-inflammatory cytokines than those in the other 2 groups (P<0.01). In the aspect of cell survival, we found the cells in the IFN-γ and LPS+IFN-γ groups had lower survival rates and higher caspase-3 expression than those in other groups (P<0.05,P<0.01).        Conclusion       N9 microglia can be polarized like macrophages and express different inflammatory cytokines after different stimulations. IFN-γ may affect the survival of N9 microglia though regulating Caspase-3 expression.

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更新日期/Last Update: 2014-09-18