[1]文玲,邓永兵,朱小云,等.5-羟色胺转运体敲除小鼠在高架十字迷宫中下丘脑CRF mRNA表达的变化[J].第三军医大学学报,2014,36(18):1876-1880.
 Wen Ling,Deng Yongbing,Zhu Xiaoyun,et al.Alteration of CRF mRNA expression in hypothalamus of SERT-knockout mice exposed to elevated plus maze[J].J Third Mil Med Univ,2014,36(18):1876-1880.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
36卷
期数:
2014年第18期
页码:
1876-1880
栏目:
论著
出版日期:
2014-09-30

文章信息/Info

Title:
Alteration of CRF mRNA expression in hypothalamus of SERT-knockout mice exposed to elevated plus maze
作者:
文玲邓永兵朱小云蒋雪
重庆医科大学生物化学与分子生物学教研室;重庆市急救医疗中心神经外科
Author(s):
Wen Ling Deng Yongbing Zhu Xiaoyun Jiang Xue

Department of Biochemistry and Molecular Biology, College of Basic Medical Sciences, Chongqing Medical University, Chongqing, 400016; Department of Neurosurgery, Emergent Medical Center, Chongqing, 400014, China

关键词:
5-羟色胺转运体高架十字迷宫下丘脑室旁核肾上腺皮质激素释放激素
Keywords:
serotonin transporterelevated plus mazehypothalamusnucleus paraventriculariscorticotrophin-releasing factor
分类号:
R338.27;R363.27;R394.3
文献标志码:
A
摘要:

目的       研究5-羟色胺转运体(serotonin transporter,SERT)敲除小鼠在高架十字迷宫(elevated plus maze, EPM)中下丘脑促肾上腺皮质激素释放因子(corticotrophin-releasing factor, CRF) mRNA表达的变化。       方法       将小鼠按基因型(SERT+/+、SERT +/-、SERT -/-)分为对照组和EPM组,每组10只,EPM组利用高架十字迷宫作为应激原刺激小鼠。测定各组小鼠下丘脑以及室旁核部位CRF mRNA的表达。       结果       在基础条件下(对照组),SERT敲除小鼠下丘脑及室旁核的CRF mRNA表达降低(P<0.05);EPM刺激下(EPM组),SERT+/-和SERT -/-小鼠在下丘脑及室旁核的CRF mRNA表达显著高于SERT+/+ (P<0.01);构建的CRF mRNA探针反义链在下丘脑PVN部位检测到高密度的CRF mRNA,而正义链却不能探测到CRF mRNA;EPM组hnRNA SERT-/-小鼠表达较对照组明显增高(P<0.01)。        结论       应激能明显促进下丘脑CRF mRNA的表达。SERT敲除小鼠的异常应激与CRF mRNA的表达升高有关。

Abstract:

Objective       To determine the expression alteration of corticotrophin-releasing factor (CRF) mRNA in the hypothalamus of serotonin transporter (SERT)-knockout mice exposed to elevated plus maze (EPM).        Methods       Mice were divided into 2 groups, a control group and an EPM group, according their genotypes (SERT+/+, SERT+/-, and SERT-/-), with 10 mice in each group. EPM was used as a stressor to stimulate the mice. Then anxious like behavior was observed, and the CRF mRNA expression in the hypothalamus and nucleus paraventricularis (PVN) were measured.        Results       The non-stress expression of CRF mRNA in the hypothalamus and PVN were lower in both SERT+/- and SERT-/- mice than in SERT+/+ mice (P<0.05). Exposure to the EPM significantly increased CFR mRNA in the hypothalamus and PVN of SERT+/- and SERT-/- mice (P<0.05), higher than that of the SERT+/+ mice. CRF mRNA antisense probes in the PVN detected high density of CRF mRNA, but CRF sense mRNA did not. With exposure to the EPM, the expression of hnRNA in SERT-/ - mice was significantly higher than that in the control group (P<0.01).        Conclusion       Stress from exposure to EPM significantly increases CRF mRNA expression in the PVN and hypothalamus of SERT+/- and SERT-/- mice. The abnormal stress reaction of SERT-knockout mice is associated with the increased CRF mRNA expression.

相似文献/References:

[1]蒋雪,邓永兵,安娜,等.5-羟色胺转运体敲除小鼠在应激下焦虑行为和HPA轴的改变[J].第三军医大学学报,2012,34(01):58.
 Jiang Xue,Deng Yongbing,An Na,et al.Change of anxious behavior and hypothalamic-pituitary-adrenal axis in serotonin transporter knockout mice under stress[J].J Third Mil Med Univ,2012,34(18):58.
[2]赵中兴,伍亚民.一种新的大鼠创伤后应激障碍模型的建立及其行为学检测[J].第三军医大学学报,2012,34(10):928.
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[3]余庆,蒋雪,李琴.焦虑模型大鼠下丘脑-垂体-肾上腺轴高反应性与糖皮质激素受体蛋白表达降低相关[J].第三军医大学学报,2017,39(14):1464.
 YU Qing,JIANG Xue,LI Qin.Hyperactivity of hypothalamus-pituitary-adrenalaxis is associated with reduced expression of glucocorticoid steroid receptor in a rat model of anxiety[J].J Third Mil Med Univ,2017,39(18):1464.

更新日期/Last Update: 2014-09-18