[1]党万太,谢文光,赵明才,等.痛风性关节炎患者NLRP3炎性体基因转录剪接体mRNA表达与临床检测指标的相关分析[J].第三军医大学学报,2014,36(16):1713-1719.
 Dang Wantai,Xie Wenguang,Zhao Mingcai,et al.Correlation analysis of expression of NLRP3 gene transcript variant mRNA and clinical examination indexes in patients with gouty arthritis[J].J Third Mil Med Univ,2014,36(16):1713-1719.
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痛风性关节炎患者NLRP3炎性体基因转录剪接体mRNA表达与临床检测指标的相关分析(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
36卷
期数:
2014年第16期
页码:
1713-1719
栏目:
论著
出版日期:
2014-08-30

文章信息/Info

Title:
Correlation analysis of expression of NLRP3 gene transcript variant mRNA and clinical examination indexes in patients with gouty arthritis
作者:
党万太谢文光赵明才蔡燕李玲琴周畅周京国
成都中医药大学临床医学院中西医结合系;川北医学院附属医院风湿免疫研究所
Author(s):
Dang Wantai Xie Wenguang Zhao Mingcai Cai Yan Li Lingqin Zhou Chang Zhou Jingguo
Department of Integrated Chinese and Western Medicines, School of Clinical Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan Province, 610075; Institute of Rheumatology and Immunology, Affiliated Hospital of North Sichuan Medical College, Sichuan Province, Nanchong 637000, China
关键词:
痛风性关节炎NLRP3炎性体基因转录剪接体
Keywords:
gouty arthritis NLRP3 inflammasome gene transcript variant
分类号:
R392.13;R446.6;R589.7
文献标志码:
A
摘要:
目的      探讨痛风性关节炎(gouty arthritis,GA)患者外周血单个核细胞(peripheral blood mononuclear cells,PBMCs)中核苷酸结合寡聚化结构域样受体3(nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing, NLRP3)炎性体基因及其转录剪接体的改变与GA患者临床实验指标的关系。      方法      用半定量聚合酶链反应RT-PCR检测44例急性痛风性关节炎(acute gouty arthritis, AGA)患者、52例非急性痛风性关节炎(non-acute gouty arthritis,NAGA)患者和50名健康体检(healthy control, HC)者PBMCs中NLRP3、凋亡相关斑点样蛋白[PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase recruitment domain (CARD), PYCARD]、半胱天冬酶1(caspase 1, CASP1)基因及其转录剪接体mRNA表达水平;检测AGA、NAGA患者与HC血常规、肝肾功能、血脂指标,比较上述3组指标的差异并分析NLRP3炎性体各基因及其转录剪接体mRNA表达与各指标的相关性。      结果      NAGA组NLRP3 mRNA表达量显著低于HC组与AGA组(P<0.05),AGA组、NAGA组NLRP3-2、3、4 mRNA表达量均显著高于HC组(P<0.05);AGA组、NAGA组PYCARD、PYCARD-1、PYCARD-2 mRNA表达量均显著高于HC组(P<0.05);AGA组CASP1 mRNA表达量显著高于HC组与NAGA组(P<0.05),AGA组CASP1-6 mRNA表达量显著低于HC组(P<0.05),NAGA组CASP1-7 mRNA表达量显著低于HC组(P<0.05),AGA组CASP1-gamma mRNA表达量显著高于HC组与NAGA组(P<0.05)。AGA组、NAGA组血常规、肝肾功能、血脂指标与HC组比较差异均有统计学意义(P<0.05);相关性分析显示NLRP3炎性体部分基因及其转录剪接体mRNA表达与AGA组、NAGA组部分临床实验指标具有相关性(P<0.05),与HC组临床实验指标均未见相关性(P>0.05)。      结论      NLRP3炎性体基因转录剪接体mRNA在GA患者中表达异常,提示在GA患者的炎症反应中NLRP3炎性体基因转录剪接体可能发挥重要的调控作用;GA患者临床实验指标与HC组比较存在差异,且NLRP3炎性体部分基因及其转录剪接体与GA患者部分临床实验指标具有相关性。
Abstract:
Objective      To study the correlation between the expression level of NLR family, pyrin domain containing 3 (NLRP3)  inflammasome gene  transcript variant mRNA and the clinical examination indexes of patients with gouty arthritis (GA).       Methods      NLRP3, PYRIN-PAAD-DAPIN domain and a C-terminal caspase recruitment domain (PYCARD) and caspase 1 (CASP1) mRNA expression in peripheral blood mononuclear cells (PBMCs) was measured by reverse transcription-polymerase chain reaction (RT-PCR). The expression of NLRP3, PYCARD and CASP1 gene mRNA in PBMCs was compared between the patients with acute gouty arthritis (AGA) (n=44), the patients with non-acute gouty arthritis (NAGA) (n=52) and healthy controls (HCs) (n=50), with β-actin as internal control. Routine blood analysis, blood biochemistry, and blood lipid indexes were measured using a routine blood analyzer and a blood biochemistry analyzer to analyze the correlation between the mRNA expression of NLRP3 inflammasome gene transcript variant and each clinical index.       Results      The mRNA expression of NLRP3 was significantly lower in the HCs and the AGA patients than in the NAGA patients (P<0.05). The mRNA expression of NLRP3-2, NLRP3-3, and NLRP3-4 increased significantly in the patients with AGA and NAGA as compared to the HCs (P<0.05). The mRNA expression of PYCARD, PYCARD-1, and PYCARD-2 increased significantly in the AGA and NAGA patients as compared to the HCs (P<0.05). The mRNA expression of CASP1 increased significantly in the AGA patients as compared to the HCs and the NAGA patients (P<0.05). The mRNA expression of CASP1-6 was significantly lower in the AGA patients as compared to the HCs (P<0.05). The mRNA expression of CASP1-7 were significantly lower in the NAGA patients compared to the HCs (P<0.05). The mRNA expression of CASP1-gamma increased significantly in the AGA patients as compared to the HCs and NAGA patients (P<0.05). There were statistically significant differences of each clinical index between the AGA and NAGA patients and the HCs (P<0.05). A positive correlation was found between the mRNA expression of NLRP3 inflammasome gene transcript variant and the clinical indexes in the AGA and NAGA patients (P<0.05), but not in the HCs (P>0.05).       Conclusion      The abnormal expression of the NLRP3 inflammasome gene and transcript variant mRNA in GA shows that the gene of NLRP3 inflammasome and its transcript variant may play a regulatory role in the inflammatory reaction. The correlation between the gene of NLRP3 inflammasome and its transcript variant and the clinical examination indexes of GA indicates the potential application in clinical diagnosis and treatment of GA.

参考文献/References:

党万太, 谢文光, 赵明才, 等. 痛风性关节炎患者NLRP3炎性体基因转录剪接体mRNA表达与临床检测指标的相关分析[J].第三军医大学学报,2014,36(16):1713-1719.

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更新日期/Last Update: 2014-08-28