[1]程哲,田驹,郑树国.Trps1调控胆管上皮细胞上皮-间质转化的实验研究[J].陆军军医大学学报(原第三军医大学学报),2014,36(08):759-763.
 Cheng Zhe,Tian Ju,Zheng Shuguo.Trps1 regulates epithelial-mesenchymal transition of intrahepatic biliary epithelial cells[J].J Amry Med Univ (J Third Mil Med Univ),2014,36(08):759-763.
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Trps1调控胆管上皮细胞上皮-间质转化的实验研究(/HTML )
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陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
36卷
期数:
2014年第08期
页码:
759-763
栏目:
论著
出版日期:
2014-04-30

文章信息/Info

Title:
Trps1 regulates epithelial-mesenchymal transition of intrahepatic biliary epithelial cells
作者:
程哲田驹郑树国
第三军医大学西南医院全军肝胆外科研究所
Author(s):
Cheng Zhe Tian Ju Zheng Shuguo
Institute of Hepatobiliary Surgery, Southwest Hospital, Third Military Medical University, Chongqing, 400038, China
关键词:
Trps1胆管上皮细胞上皮-间质转化冷保存再灌注损伤
Keywords:
Trps1 biliary epithelial cells epithelial-mesenchymal transition cold preservation reperfusion injury
分类号:
R-331;R394.3;R657.4
文献标志码:
A
摘要:
目的      探讨毛发鼻指(趾)综合征1型相关基因(tricho-rhino-pharyngeal syndrome type 1,Trps1)在冷保存再灌注损伤(cold preservation reperfusion injury,CPRI)诱导人肝内胆管上皮细胞(human intrahepatic biliary epithelial cells,HIBECs)上皮-间质转化(epithelial-mesenchymal transition,EMT)中的作用。      方法      体外模拟CPRI处理HIBECs检测上皮标志物E-cadherin、CK19,间质标志物Vimentin、α-SMA及目标基因Trps1的表达。用Trps1腺病毒或Trps1 siRNA载体转染HIBECs后,重复CPRI诱导实验,探讨Trps1对HIBECs EMT的调控作用。      结果      随着冷保存时间的延长,上皮标志物E-cadherin、CK19 mRNA和蛋白表达持续减少(P=0.018,0.011;P=0.039,0.044),间质标志物Vimentin、α-SMA表达持续增加(P=0.002,0.026;P=0.035,0.016);冷保存12 h,细胞内Trps1 mRNA和蛋白表达增加(P=0.018,0.031),其后随冷保存时间延长持续减少(P=0.026,0.009)。相关分析显示在该过程中,Trps1与上皮标志物表达正相关(r=0.981,0.913;P=0.000,0.002),而与间质标志物表达负相关(r=-0.711,-0.847; P=0.009, 0.005)。 Trps1腺病毒转染HIBEC 后,重复上述CPRI诱导实验,E-cadherin表达增加(P=0.002,0.000),Vimentin表达减少(P=0.000,0.017),CPRI诱导HIBECs EMT效应受到抑制;Trps1 siRNA转染HIBECs后,重复上述CPRI诱导实验,E-cadherin表达减少(P=0.032,0.044),Vimentin表达增加(P=0.047,0.031),加强CPRI诱导HIBECs EMT效应。      结论      体外模拟CPRI可诱导HIBECs发生EMT,Trps1参与调控HIBECs EMT,发挥重要的拮抗作用并可能逆转该过程。
Abstract:
Objective      To observe the epithelial-mesenchymal transition (EMT) of human intrahepatic biliary epithelial cells (HIBECs) induced by cold preservation reperfusion injury (CPRI), and to study the role of tricho-rhino-pharyngeal syndrome type 1 (Trps1) in the occurrence of HIBECs EMT.       Methods      HIBECs were incubated with simulated CPR1 in vitro, then expressions of E-cadherin, CK19, Vimentin, α-SMA and the target gene Trps1 in HIBECs were detected by RT-PCR and Western blotting after treatment of HIBECs with CPRI.  The effect of Trps1 was increased with recombinant adenovirus vector containing Trps1 gene or blocked with Trps1 siRNA, then the role of Trps1 was studied.       Results      With the prolongation of cold preservation time, the mRNA and protein expression of EMT markers, E-cadherin and CK19 was decreased (P=0.018, 0.011; P=0.039, 0.044), while that of Vimentin and α-SMA was significantly increased (P=0.002, 0.026; P=0.035, 0.016). The expression of Trps1 mRNA and protein reached its peak at 12 h (P=0.018, 0.031) and began to decrease at 24 and 48 h, respectively (P=0.026, 0.009). Trps1 was positively correlated with the epithelial markers (r=0.981, 0.913; P=0.000, 0.002) but negatively correlated with the mesenchymal markers (r=-0.711, -0.847; P=0.009, 0.005). Recombinant adenovirus vector containing Trps1 gene could up-regulate the CPRI-induced expression decrease of E-cadherin (P=0.002, 0.000) but down-regulate the expression increase of Vimentin (P=0.000, 0.017). Trps1 siRNA could down-regulate the CPRI-induced expression decrease of E-cadherin (P=0.032, 0.044) but up-regulate the expression increase of Vimentin and α-SMA (P=0.047, 0.031).       Conclusion      CPRI can induce the occurrence of HIBECs EMT. Trps1 participates in the regulation of HIBECs EMT as a key antagonistic factor, and may reverse the process.

相似文献/References:

[1]黄坤,张建国,李开龙,等.Trps1在大鼠肾脏缺血再灌注损伤修复过程中的表达及意义[J].陆军军医大学学报(原第三军医大学学报),2012,34(12):1158.
 Huang Kun,Zhang Jianguo,Li Kailong,et al.Expression and significance of Trps1 in rat kidney repair after ischemia/reperfusion injury[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(08):1158.

更新日期/Last Update: 2014-04-21