[1]郑波,杨源瑞,周振华,等.PPAR-γ通过PI3K/Akt及NF-κB通路调控猪颈动脉支架植入术后的血管重构[J].第三军医大学学报,2014,36(09):888-892.
 Zheng Bo,Yang Yuanrui,Zhou Zhenhua,et al.PPAR-γ regulates vascular remodeling after pig carotid artery stent implantation through PI3K/Akt and NF-κB pathway[J].J Third Mil Med Univ,2014,36(09):888-892.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
36卷
期数:
2014年第09期
页码:
888-892
栏目:
论著
出版日期:
2014-05-15

文章信息/Info

Title:
PPAR-γ regulates vascular remodeling after pig carotid artery stent implantation through PI3K/Akt and NF-κB pathway
作者:
郑波杨源瑞周振华吴虹辰刘渠陈康宁
第三军医大学西南医院神经内科
Author(s):
Zheng Bo Yang Yuanrui Zhou Zhenhua Wu Hongchen Liu Qu Chen Kangning
Department of Neurology, Southwest Hospital, Third Military Medical University, Chongqing, 400038, China
关键词:
过氧化物酶体增殖物激活受体-γAktp-AktNF-κB血管重构
Keywords:
peroxisome proliferator activated receptors-γ Akt p-Akt NF-κB vascular remodeling swine
分类号:
R322.121; R363.22; R394.3
文献标志码:
A
摘要:
目的      观察过氧化物酶体增殖物激活受体-γ(peroxisome proliferator activated  receptors-γ,PPAR-γ)在猪颈动脉支架植入术后血管重构中的作用。      方法        将12只小型猪分为正常对照组(n=4)和介入手术组(n=8),介入手术组采用猪颈动脉支架植入建立动物模型,分为支架组(n=4)和罗格列酮(ROSI)组(n=4)。术后3个月,应用数字减影血管造影(digital subtraction angiography,DSA)技术及HE染色评估血管形态学变化,应用免疫组织化学方法检测p-Akt及NF-κB的表达变化,应用Western blot方法检测支架段血管PPAR-γ、Akt、p-Akt及NF-κB的表达变化。      结果        ①猪颈动脉DSA和HE染色结果发现支架组血管狭窄较ROSI组明显(P<0.01)。②免疫组织化学染色结果发现支架组颈动脉血管p-Akt和NF-κB表达较正常对照组明显增加(P<0.01),ROSI组颈动脉血管p-Akt和NF-κB表达较支架组明显降低(P<0.01)。③Western  blot检测结果显示:支架组PPAR-γ蛋白表达水平较正常对照组明显降低(P<0.01),NF-κB和p-Akt蛋白表达水平较正常对照组明显增加(P<0.05);ROSI组PPAR-γ蛋白表达水平较支架组明显增加(P<0.01),NF-κB和p-Akt蛋白表达水平较支架组明显降低(P<0.01);Akt在正常对照组、支架组和ROSI组中的蛋白表达量差异无统计学意义(P>0.05)。      结论        PPAR-γ可能通过调节PI3K/Akt及NF-κB的表达,从而改善支架植入术后的血管重构。
Abstract:
Objective      To determine the role of peroxisome proliferator activated receptors-γ (PPAR-γ) in swine vascular remodeling after carotid artery stent implantation.       Methods        Totally 12 miniature pigs were randomly divided into normal control group (n=4) and intervention group (n=8). The animals of intervention group were adopted to establish the animal model of carotid artery stenting, and then randomly divided into stent subgroup (n=4) and rosiglitazone treatment (ROSI) subgroup (n=4). In 3 months after surgery, digital subtraction angiography (DSA) and HE staining was used to assess vascular morphology, immunohistochemical staining was applied to detect the expression of P-akt and NF-κB, and Western blotting was employed to detect the expression of PPAR-γ, Akt, p-Akt and NF-κB.       Results        DSA and HE staining demonstrated that vascular stenosis was more severe in the stent subgroup than the ROSI group (P<0.01). Immunohistochemical staining showed that the expression of p-Akt and NF-κB were significantly increased in the carotid artery stent groups than normal control group (P<0.01), and that in ROSI subgroup was significantly lower than in the stent subgroup (P<0.01). Western blot analysis showed that the protein expression level of PPAR-γ was significantly lower (P<0.01), and those of NF-κB and p-Akt were obviously higher in the stent subgroup than the normal control group (P<0.05), while that of PPAR-γ was significantly enhanced (P<0.01), and those of NF-κB and p-Akt were markedly reduced in the ROSI subgroup than in the stent subgroup (P<0.01). However, there was no significant difference in the protein expression of Akt in normal control group, stent subgroup and ROSI subgroup (P>0.05).       Conclusion        PPAR-γ might mediate the expression of PI3K/Akt and NF-κB, and then improve the vascular remodeling after stent implantation.

参考文献/References:

郑波, 杨源瑞, 周振华, 等. PPAR-γ通过PI3K/Akt及NF-κB通路调控猪颈动脉支架植入术后的血管重构[J].第三军医大学学报,2014,36(9):888-892.

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更新日期/Last Update: 2014-05-05