[1]赵海,马婧,卢俊杰,等.γ-氨基丁酸通过其A型受体参与小鼠母胎血管的形成研究[J].陆军军医大学学报(原第三军医大学学报),2014,36(12):1237-1242.
 Zhao Hai,Ma Jing,Lu Junjie,et al.Gamma-aminobutyric acid participates in mouse feto-maternal vasculogenesis through GABA type A receptor[J].J Amry Med Univ (J Third Mil Med Univ),2014,36(12):1237-1242.
点击复制

γ-氨基丁酸通过其A型受体参与小鼠母胎血管的形成研究(/HTML )
分享到:

陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
36卷
期数:
2014年第12期
页码:
1237-1242
栏目:
论著
出版日期:
2014-06-30

文章信息/Info

Title:
Gamma-aminobutyric acid participates in mouse feto-maternal vasculogenesis through GABA type A receptor
作者:
赵海马婧卢俊杰谭冬梅张倩罗文萍谭毅
重庆医科大学实验动物中心
Author(s):
Zhao Hai Ma Jing Lu Junjie Tan Dongmei Zhang Qian Luo Wenping Tan Yi
Laboratory Animal Center, Chongqing Medical University, Chongqing, 400016, China
关键词:
γ-氨基丁酸蜕膜化迷路区胎盘
Keywords:
γ-aminobutyric acid decidualization labyrinth placenta
分类号:
R321.4;R322.12;R341.7
文献标志码:
A
摘要:
目的      探讨不同浓度的γ-氨基丁酸 (γ-aminobutyric acid,GABA) A型受体激动剂/抑制剂对小鼠胎盘形成的影响。      方法      免疫组化检测孕鼠中晚期胎盘中GABA的A型受体(GABAAR)π亚基(GABRP)的表达规律。将昆明孕鼠分为8组,每组3只,处理组从妊娠第7~13天分别腹腔注射0.009 5、 0.095 mg/kg及0.95 mg/kg A型受体激动剂和0.018 4、0.184 mg/kg 及1.84 mg/kg的A型受体抑制剂溶液,对照组注射生理盐水,空白组不作任何处理。第14天时称量各组胎盘和胚胎,HE染色观察胎盘形态学变化,检测增殖细胞核抗原(proliferating cell nuclear antigen, PCNA)及血管内皮生长因子(vascular endothelial growth factor, VEGF)表达情况。      结果      GABRP强表达于孕鼠第10~17天的母胎蜕膜区;与对照组比较,0.95 mg/kg GABAAR激动剂组蜕膜区血管数量和胎盘迷路区胎儿有核红细胞的数量显著增加(P<0.05),同时迷路区PCNA和VEGF的表达显著增加(P<0.05);1.84 mg/kg GABAAR抑制剂组胎盘和胚胎的质量显著降低(P<0.05),同时迷路区PCNA和VEGF表达均降低。      结论      GABA可能通过与其A型受体结合参与调节小鼠胎盘增殖和血管的生成过程。
Abstract:
Objective      To study the potential roles of γ-aminobutyric acid(GABA) type A receptor (GABAAR) agonist and antagonist in mouse placentation.       Methods      Immunohistochemistry was applied to detect the expression of the π subunit of GABAAR (GABRP) in the placentas of mid and late phase during mouse pregnancy. Kunming pregnant mice were divided into 8 groups (n=3) randomly, including 3 treatment groups with daily intraperitoneal injection of GABAAR agonist of 0.009 5, 0.095, and 0.95 mg/kg separately from D7 to D13, 3 treatment groups with daily intraperitoneal injection of GABAAR antagonist of 0.018 4, 0.184, and 1.84 mg/kg separately from D7 to D13, a control group with injection of saline solution, and a blank group without any treatment. The weights of embryos and placentas were measured and the structure of placenta was observed by HE staining on D14. Besides, the state of proliferation and vasculogenesis in the placenta were evaluated by the expression levels of proliferating cell nuclear antigen (PCNA) and vascular endothelial growth factor (VEGF) using immunohistochemical assay.       Results      GABRP was mainly expressed in the decidua basalis of the uterus from D10 to D17 in pregnant rats. The numbers of blood vessels in the placental decidual zone and nucleated red cells in the labyrinth were significantly increased in the 0.95 mg/kg GABAAR agonist group (P<0.05), while the expression of PCNA and VEGF was also significantly increased in the placenta labyrinth (P<0.05). Surprisingly, the weights of placenta and embryo were significantly decreased in the 1.84 mg/kg GABAAR antagonist group as compared to the control group (P<0.05), while the expression of PCNA and VEGF was reduced in the placenta labyrinth.       Conclusion      GABA may participate in mouse placentation and vasculogenesis via GABAAR.

相似文献/References:

[1]欧阳俊彦,胡卓炎,褚玥,等.γ-氨基丁酸对情绪应激大鼠额叶皮质一氧化氮合酶和一氧化氮水平的影响[J].陆军军医大学学报(原第三军医大学学报),2013,35(05):385.
 Ouyang Junyan,Hu Zhuoyan,Chu Yue,et al.Modulation of emotional stress-induced NOS and NO changes in rat frontal cortex by γ-aminobutyric acid[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(12):385.
[2]何建荣,陈丽峰,李瑶琛.视网膜变性对RCS-P+大鼠γ-氨基丁酸及其合成酶谷氨酸脱羧酶65表达的影响[J].陆军军医大学学报(原第三军医大学学报),2013,35(05):390.
 He Jianrong,Chen Lifeng,Li Yaochen.Effect of retinal degeneration on expression of γ-aminobutyric acid and glutamic acid decarboxylase 65 in RCS-P+ rats[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(12):390.
[3]张映琦,廖维宏,迟路湘,等.癫痫状态大鼠海马GABA、GABAA受体α5亚单位表达的动态变化[J].陆军军医大学学报(原第三军医大学学报),2006,28(20):2047.
[4]史良会,张才全.γ-氨基丁酸及其A型受体在胃癌SGC-7901细胞的表达及对细胞增殖的影响[J].陆军军医大学学报(原第三军医大学学报),2011,33(20):2132.
 Shi Lianghui,Zhang Caiquan.Expression of GABA and GABRP in gastric cancer SGC-7901 cells and its effect on cell migration ability[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(12):2132.
[5]汪绪武,孔维佳.大鼠前庭内侧核γ-氨基丁酸受体mRNA的表达[J].陆军军医大学学报(原第三军医大学学报),2005,27(21):2124.
[6]陈康宁,瞿伟,覃园园,等.焦虑抑郁障碍患者ET的变化[J].陆军军医大学学报(原第三军医大学学报),2004,26(16):0.[doi:10.16016/j.1000-5404.2004.16.002 ]
[7]毕晓宁,刘祚周,廖维宏,等.侧脑室注射GABA、PTX对猫背海马神经元伤害性放电的影响[J].陆军军医大学学报(原第三军医大学学报),1989,11(01):0.[doi:10.16016/j.1000-5404.1989.01.003 ]
 Bi Xiaoning,et al.[J].J Amry Med Univ (J Third Mil Med Univ),1989,11(12):0.[doi:10.16016/j.1000-5404.1989.01.003 ]
[8]黎海蒂,张问德.安定加强小鼠的促肾上腺皮质激素镇痛作用[J].陆军军医大学学报(原第三军医大学学报),1989,11(03):0.[doi:10.16016/j.1000-5404.1989.03.008 ]
 Li Haiti and Zhang Wende.[J].J Amry Med Univ (J Third Mil Med Univ),1989,11(12):0.[doi:10.16016/j.1000-5404.1989.03.008 ]

更新日期/Last Update: 2014-06-20