[1]赵德华,卢来春,管海燕,等.牛蒡子苷自微乳在体肠吸收分析方法学研究[J].陆军军医大学学报(原第三军医大学学报),2013,35(24 ):2681-2685.
 Zhao Dehua,Lu Laichun,Guan Haiyan,et al.Analytical methodology on in situ rat intestine absorption of arctiin SMEDDS[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(24 ):2681-2685.
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陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
35卷
期数:
2013年第24期
页码:
2681-2685
栏目:
论著
出版日期:
2013-12-30

文章信息/Info

Title:
Analytical methodology on in situ rat intestine absorption of arctiin SMEDDS
作者:
赵德华卢来春管海燕张卿尚京川
重庆医科大学药学院药物分析教研室;第三军医大学大坪医院野战外科研究所药剂科
Author(s):
Zhao Dehua Lu Laichun Guan Haiyan Zhang Qing Shang Jingchuan
Department of Pharmaceutical Analysis, School of Pharmacy, Chongqing Medical University, Chongqing, 400016; Department of Pharmacy, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing, 400042, China
关键词:
HPLC牛蒡子苷自微乳在体肠吸收含量测定
Keywords:
HPLC arctiin SMEDDS in situ perfusion content determination
分类号:
R282.71;R944.9;R965
文献标志码:
A
摘要:
目的      建立HPLC法测定牛蒡子苷自微乳中牛蒡子苷含量并考察牛蒡子苷自微乳在大鼠小肠吸收情况。      方法      采用自微乳化释药系统(SMEDDS)技术制备牛蒡子苷自微乳,并运用大鼠在体灌流的方法建立牛蒡子苷大鼠肠吸收模型,以流动相乙腈-水(35∶65),流速1 mL/min,检测波长280 nm为色谱条件,测定自微乳中牛蒡子苷含量及肠循环液中牛蒡子苷、苷元含量,并考察药物的吸收。      结果      牛蒡子苷含量测定方法在2~200 μg/mL浓度范围内,苷元含量测定方法在1~100 μg/mL浓度范围内,均具有良好线性,回收率均在95%~105%范围内,日内及日间RSD均小于5%。测得牛蒡子苷自微乳载药量为(40.090±0.001)mg/g;在体肠吸收结果表明在10~50 μg/mL范围内,药物单位时间吸收百分率小于25%;牛蒡子苷自微乳在小肠有一定的吸收,但在30 min后开始水解为牛蒡子苷元。      结论      所建HPLC法专属性强,准确度及精密度能够满足牛蒡子苷自微乳以及在体肠吸收液中牛蒡子苷、苷元含量的测定。
Abstract:
Objective      To establish an HPLC method for determination of arctiin in self-microemulsifying drug delivery system (SMEDDS) and studying its absorption in rat intestines.       Methods      An in situ recirculation model was established to study the absorption of arctiin SMEDDS in rat intestines. The contents of arctiin and arctigenin and drug absorption were determined by HPLC, with mobile phase of acetonitrile-water (35∶65), flow rate 1 mL/min, and detection wavelength 248 nm.       Results      The method showed good linearity in arctiin concentration range of 2-200 μg/mL and arctigenin concentration range of 1-100 μg/mL, with recovery rate 95% to 105% and inter-day and intra-day RSD less than 5%. The content of arctiin in microemulsion was (40.090±0.001) mg/g. In the concentration range from 10 to 50 μg/mL, arctiin absorption rate was less than 25%. The arctiin SMEDDS had some absorption in rat intestines but started to hydrolyze and produce arctigenin after 30 min.       Conclusion      The HPLC method with good specificity, accuracy and precision is qualified for determination of arctiin and arctigenin from SMEDDS and intestinal fluids.

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更新日期/Last Update: 2013-12-18