[1]陈军,崔有宏,郝迎学,等.5-氟尿嘧啶及长春新碱对胃癌细胞SGC-7901干细胞相关特性的影响[J].陆军军医大学学报(原第三军医大学学报),2013,35(12):1247-1251.
 Chen Jun,Cui Youhong,Hao Yingxue,et al.Effects of 5-FU and vincristine on stem cell-associated phenotypes and properties of gastric cancer cell line SGC-7901[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(12):1247-1251.
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5-氟尿嘧啶及长春新碱对胃癌细胞SGC-7901干细胞相关特性的影响(/HTML )
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陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
35卷
期数:
2013年第12期
页码:
1247-1251
栏目:
论著
出版日期:
2013-06-30

文章信息/Info

Title:
Effects of 5-FU and vincristine on stem cell-associated phenotypes and properties of gastric cancer cell line SGC-7901
作者:
陈军崔有宏郝迎学唐波植懿丹余佩武
第三军医大学:西南医院全军普通外科中心,微创胃肠外科中心,西南医院病理学研究所,基础医学部全军免疫学研究所
Author(s):
Chen Jun Cui Youhong Hao Yingxue Tang Bo Zhi Yidan Yu Peiwu
Center of General Surgery, Institute of Pathology, Southwest Hospital, Institute of Immunology, College of Basic Medical Sciences, Third Military Medical University,  Chongqing, 400038, China
关键词:
胃癌干细胞5-FU长春新碱上皮-间质转化
Keywords:
gastric cancer stem cells 5-FU vincristine epithelial-to-mesenchymal transition
分类号:
R73-361;R735.2;R979.1
文献标志码:
A
摘要:
目的      探讨化疗药物5-FU、长春新碱对胃癌细胞SGC-7901干细胞相关表型及功能影响。      方法      5-FU、长春新碱分别作用于SGC-7901细胞后,通过流式细胞术及荧光实时定量PCR的方法观察胃癌干细胞候选标志CD44、CD133的表达,检测干性相关基因及上皮-间质转化(epithelial-to-mesenchymal transition,EMT)相关基因的表达变化,并检测药物作用对其集落形成能力、无血清培养基中成球能力及NOD/SCID小鼠体内成瘤能力的影响。      结果      不同浓度5-FU、长春新碱作用3 d后均可显著增加剩余SGC-7901细胞CD44 mRNA的总体表达水平,分别为未用药组的(2.36±0.03)、(1.74±0.05)、(6.03±0.75)、(4.06±0.77)倍,均有显著差异(P<0.05)。CD133及CD44阳性细胞比例亦明显升高,但干性基因表达、克隆形成能力、成瘤能力未见明显增加。而与5-FU不同的是,长春新碱作用后细胞发生了EMT相关基因的表达改变,在无血清培养基中形态与其他组细胞差异显著,且可形成典型的细胞球。      结论      5-FU及长春新碱作用后细胞CD44和CD133的表达增加,此外长春新碱还促进细胞EMT改变,并增加细胞在无血清培养基中的成球能力。
Abstract:
Objective      To identify the effects of 5-fluorouracil (5-FU) and vincristine (VCR) on stem cell-associated phenotypes and properties of gastric cancer cell line SGC-7901.       Methods      SGC-7901 cells were treated with 5-FU and VCR, respectively. Candidate stem cell markers of CD44 and CD133 as well as other stemness genes and epithelial-to-mesenchymal transition (EMT)-associated genes were assayed. Colony formation assay, sphere formation assay and tumorigenic formation assay were used to test the properties of SGC-7901 cells associated with cancer stem cells.       Results      After 5-FU and VCR treatment, the mRNA expression levels of CD44 and CD133 significantly increased in all the doses as compared with the control group (up to 2.36±0.03, 1.74±0.05, 6.03±0.75 and 4.06±0.77 folds respectively, P<0.05), but the capabilities of colony formation and tumorigenic formation had no change. VCR treatment manifested the EMT gene profile and showed different morphologic features in serum-free SGC-7901 cell culture, which was different from 5-FU treatment.       Conclusion      Transient treatment of 5-FU and VCR up-regulates the expression of CD44 and CD133. VCR induces EMT changes in SGC-7901 cells and enhances sphere formation in serum-free culture medium.

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更新日期/Last Update: 2013-06-20