[1]杨先涛,张祯祯,詹学,等.肺结核患儿外周血单个核细胞中microRNA表达的初步研究[J].陆军军医大学学报(原第三军医大学学报),2013,35(19):2051-2055.
　Yang Xiantao,Zhang Zhenzhen,Zhan Xue,et al.microRNA expression in peripheral blood mononuclear cells from pulmonary tuberculosis children[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(19):2051-2055.
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肺结核患儿外周血单个核细胞中microRNA表达的初步研究(/HTML )
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陆军军医大学学报（原第三军医大学学报）[ISSN:1000-5404/CN:51-1095/R]

卷:
35卷
期数:
2013年第19期
页码:
2051-2055
栏目:
论著
出版日期:
2013-10-15

文章信息/Info

Title:
microRNA expression in peripheral blood mononuclear cells from pulmonary tuberculosis children
作者:
杨先涛; 张祯祯; 詹学; 朱朝敏
重庆医科大学附属儿童医院感染科，儿童发育疾病研究教育部重点实验室，儿科学重庆市重点实验室，重庆市儿童发育重大疾病诊治与预防国际科技合作基地
Author(s):
Yang Xiantao;  Zhang Zhenzhen;  Zhan Xue;  Zhu Chaomin
Department of Infectious Diseases, Key Laboratory of Child Development and Disorders of Ministry of Education, Chongqing Key Laboratory of Pediatrics, Chongqing International Science and Technology Cooperation Center for Child Development and Disorders, Children’s Hospital, Chongqing Medical University, Chongqing, 400014, China
关键词:
儿童; 肺结核; microRNA; 诊断; 外周血单个核细胞
Keywords:
children;  pulmonary tuberculosis;  microRNA;  diagnosis;  peripheral blood mononuclear cells
分类号:
R392.12; R446.9; R529.9
文献标志码:
A
摘要:
目的      探讨肺结核患儿与健康儿童外周血单核细胞中microRNA表达水平的差异及在儿童肺结核中的诊断价值。      方法       提取肺结核及健康儿童外周血单个核细胞（peripheral blood mononuclear cell，PBMC）中RNA，进行microRNA芯片检测，筛选出差异表达的microRNA，并用实时荧光定量PCR进行验证，运用t检验对结果进行分析，对目标microRNA进行生物信息学初步分析。      结果       通过microRNA芯片检测到21个差异表达的microRNA，15个表达上调，6个表达下调，最终选取6个microRNA（hsa-miR-193a-3p、hsa-miR-144、hsa-miR-342-5p、hsa-miR-31、hsa-miR-33a、hsa-miR-142-3p）。经实时荧光定量PCR验证，与正常组相比，肺结核患儿PBMC中hsa-miR-193a-3p、hsa-miR-144表达上调（P<0.05），hsa-miR-342-5p、hsa-miR-31表达下调(P<0.05)。生物信息学初步分析提示4种microRNA在结核与宿主细胞相互作用的相关信号通路中可能有发挥一定的作用。      结论       与成人肺结核中microRNA表达相比，儿童肺结核有其特异表达的microRNA，并可能参与结核病发生、发展的相关的一些信号通路，可作为儿童肺结核潜在的诊断价值。
Abstract:
Objective      To determine the expression differences of microRNAs in the peripheral blood mononuclear cells (PBMCs) between children with pulmonary tuberculosis (PTB) and healthy controls in order to evaluate the potential value of microRNAs in PTB diagnosis.       Methods      Total RNAs were extracted from the PBMCs derived from the blood of PTB children and healthy controls. Human microarrays were employed to screen the microRNAs that were differently expressed, and then RT-qPCR was carried out to qualify the expression levels. Students’ t-test was applied to screen out the microRNAs with deferential expression levels. Bioinformatics analysis was performed to understand microRNA-mRNA interaction network.       Results      Analysis of expression profiles showed that the expression of 21 microRNAs was significantly altered in the PBMCs from PTB children compared with healthy control. Among them, 15 microRNAs were up-regulated and 6 were down-regulated. RT-qPCR indicated that among the 6 microRNAs we chose (hsa-miR-193a-3p, hsa-miR-144, hsa-miR-342-5p, hsa-miR-31, hsa-miR-33a and hsa-miR-142-3p), hsa-miR-193a-3p and hsa-miR-144 were upregulated (P<0.05), while hsa-miR-342-5p and hsa-miR-31 were downregulted in the PTB children (P<0.05). Bioinformatics analysis showed that these 4 microRNAs played certain roles in the signalling circuits in molecular interaction networks interactional signal pathway networks between PTB  and the host cells.       Conclusion      Compared with adult tuberculosis microRNA expressions,childhood pulmonary tuberculosis shows some different performances and has some correlations with some signaling pathway and these microRNAs might be considered as potential biomarkers of childhood PTB diagnosis.

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更新日期/Last Update: 2013-09-30