[1]蔡安季,戴勇,李武县,等.强直性脊柱炎患者外周血单个核细胞中急性时相反应蛋白的差异表达研究[J].陆军军医大学学报(原第三军医大学学报),2013,35(19):2084-2087.
 Cai Anji,Dai Yong,Li Wuxian,et al.Expression of acute phase proteins in mononuclear cells from ankylosing spondylitis patients[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(19):2084-2087.
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强直性脊柱炎患者外周血单个核细胞中急性时相反应蛋白的差异表达研究(/HTML )
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陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
35卷
期数:
2013年第19期
页码:
2084-2087
栏目:
论著
出版日期:
2013-10-15

文章信息/Info

Title:
Expression of acute phase proteins in mononuclear cells from ankylosing spondylitis patients
作者:
蔡安季戴勇李武县李紫微涂植光
重庆医科大学检验医学院教育部临床检验诊断学重点实验室
Author(s):
Cai Anji Dai Yong Li Wuxian Li Ziwei Tu Zhiguang
Key Laboratory of Laboratory Medical Diagnostics of Ministry of Education, College of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, China
关键词:
强直性脊柱炎外周血单个核细胞 急性时候反应蛋白差异表达相对和绝对定量的等量异位标签技术
Keywords:
ankylosing spondylitis peripheral blood mononuclear cells acute phase proteins differential expression isobaric tags for relative and absolute quantitation
分类号:
R446.112; R446.9; R593.23
文献标志码:
A
摘要:
目的      检测强直性脊柱炎外周血单个核细胞中急性时相反应蛋白表达水平,探讨其与强直性脊柱炎发病的关系。      方法       收集10例强直性脊柱炎患者和9例健康对照,提取外周血单个核细胞,采用相对和绝对定量的等量异位标签串联质谱技术检测强直性脊柱炎患者外周血单个核细胞中的急性时相反应蛋白表达水平,并运用Western blot方法进行验证差异表达的触珠蛋白(Hp)和铜蓝蛋白(Cp)。      结果       基于质谱分析的蛋白组学显示,19种急性时相反应蛋白在强直性脊柱炎外周血单个核细胞中蛋白表达水平明显升高(P<0.05),主要包括补体C3、纤粘蛋白(FN1)、血浆酶原(PLG)、转铁蛋白(TF)、Hp和Cp;Western blot检测Hp和Cp的表达水平与相对和绝对定量的等量异位标签串联质谱技术检测结果具有相同的变化趋势。      结论       急性时相反应蛋白差异表达可能参与了强直性脊柱炎的发生、发展,有望作为强直性脊柱炎的临床生物标记物。
Abstract:
Objective      To determine the differential expression levels of acute phase proteins (APPs) in peripheral blood mononuclear cells (PBMCs) from patients with ankylosing spondylitis (AS), and to investigate their role in the pathogenesis of AS.       Methods      PBMCs were isolated from 9 health controls and 10 AS patients by using Ficoll-Paque plus solution. The proteins of PBMCs were extracted using cell lysate, and iTRAQ coupled with multiple chromatographic fractionation and tandem mass spectrometry were used to screen the APPs in the obtained PBMCs. Mascot software (Matrix Science) was applied to identify and quantify the proteins, and Western blotting were used to further validate the expression levels of haptoglobin (Hp) and ceruloplasmin (Cp).       Results      Initial mass spectrometry-based proteomic analysis revealed that the expression levels of 19 APPs were obviously increased in the PBMCs from AS patients (P<0.05), mainly including complement C3, isoform 1 of fibronectin, plasminogen, serotransferrin, Hp and Cp. Moreover, Western blotting indicated that the expression levels of Hp and Cp had a similar trend with iTRAQ results.       Conclusion      The differential expression of APPs might be associated with the pathologenesis and development of AS, and can be used as clinical biomarkers of AS.

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更新日期/Last Update: 2013-09-30