[1]周丽蓉,张劼,罗永艾.IDO与Treg在支气管哮喘小鼠中的相互作用及其意义[J].第三军医大学学报,2013,35(13):1384-1387.
 Zhou Lirong,Zhang Jie,Lou Yongai.Interaction of indoleamine 2, 3-dioxygenase and CD4+CD25+Foxp3+ regulatory T cell in asthmatic mice[J].J Third Mil Med Univ,2013,35(13):1384-1387.
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IDO与Treg在支气管哮喘小鼠中的相互作用及其意义(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
35卷
期数:
2013年第13期
页码:
1384-1387
栏目:
论著
出版日期:
2013-07-15

文章信息/Info

Title:
Interaction of indoleamine 2, 3-dioxygenase and CD4+CD25+Foxp3+ regulatory T cell in asthmatic mice
作者:
周丽蓉张劼罗永艾
重庆医科大学附属第一医院呼吸内科;重庆市第三人民医院老年科
Author(s):
Zhou Lirong Zhang Jie Lou Yong’ai
Department of Respiratory Diseases, First Affiliated Hospital, Chongqing Medical University, Chongqing, 400016; Department of Geriatrics, Third People’s Hospital of Chongqing, Chongqing, 400014, China
关键词:
吲哚胺23双加氧酶调节性T细胞支气管哮喘实时荧光定量PCR流式细胞术
Keywords:
indoleamine 23-dioxygenase regulatory T cell asthma real-time fluorescence-based quantitative PCR flow cytometric analysis
分类号:
R392.32; R345; R562.25
文献标志码:
A
摘要:
目的      探讨吲哚胺2,3双加氧酶(indoleamine 2,3-dioxygense,IDO) 与CD4+CD25+Foxp3+调节性T细胞(Treg)之间的相关性及在支气管哮喘发病机制中的作用。      方法       BALB/c小鼠用随机数字表法分成对照组和哮喘组,每组8只。哮喘组以鸡卵清蛋白(ovalbumin ,OVA)致敏,激发小鼠建立哮喘模型,无创肺功能仪检测气道反应性,支气管肺泡灌洗液(BALF)进行细胞学分析,ELISA检测BALF中INF-γ、IL-4、IL-10浓度,实时荧光定量PCR检测肺组织IDO和Foxp3 mRNA表达,免疫组织化学方法检测IDO蛋白表达,流式细胞仪检测Treg占CD4+细胞的百分率。      结果       哮喘组小鼠气道反应性、BALF中细胞总数、嗜酸性粒细胞比例及IL-4浓度明显高于对照组(P<0.01);而INF-γ与IL-10浓度、IDO和Foxp3的mRNA表达、IDO蛋白表达、Treg占CD4+细胞的百分率明显低于对照组(P<0.01);对照组与哮喘组IDO与Foxp3的mRNA表达呈正相关(r=0.819,r=0.807,P<0.05),对照组与哮喘组IDO蛋白表达与Treg占CD4+细胞的百分率呈正相关(r=0.783,r=0.765,P<0.05)。      结论       哮喘小鼠IDO和Foxp3表达降低,Treg数量减少,且IDO蛋白表达与Treg占CD4+细胞的百分率呈正相关,表明IDO与Treg相互调节,打破免疫耐受,诱导哮喘发生。
Abstract:
Objective       To explore the interaction and the role of indoleamine 2,3-dioxygenase (IDO) and CD4+CD25+Foxp3+ regulatory T cell (Treg) in a mice model of allergic bronchial asthma.       Methods       BALB/c mice were sensitized and challenged by ovalbumin (OVA). Penh were measured to evaluate the airway responsiveness by noninvasive lung functional instrument. Bronchoalveolar lavage cytology was analyzed. IFN-γ, IL-4 and IL-10 in BALF were detected by enzyme-linked immunosorbent assay (ELISA). The mRNA expression of IDO and Foxp3 was measured by real-time fluorescence-based quantitative PCR. The protein expression of IDO was detected by immunohistochemistry. The percentage of Treg in CD4+ cells was assessed by flow cytometry.       Results       The airway responsiveness, the total cell number, the eosinophils and IL-4 in BALF of the asthmatic group significantly increased as compared with the control group (P<0.01). The levels of IFN-γ and IL-10 in BALF, the mRNA expression of IDO and Foxp3, the protein expression of IDO, and the percentage of Treg in CD4+ cells in the asthmatic group were significantly lower than those in the control group (P<0.01). The mRNA expression of IDO and Foxp3 was positively correlated with each other (r=0.819, 0.807, P<0.05). The protein expression of IDO was positively correlated with the percentage of Treg in CD4+cells (r=0.783, 0.765, P<0.05).       Conclusions       IDO and Treg reciprocally regulate each other, which surmounts immune tolerance and induces asthma. Therefore, IDO and Treg may play important roles in asthma.

参考文献/References:

周丽蓉,张劼,罗永艾. IDO与Treg在支气管哮喘小鼠中的相互作用及其意义[J].第三军医大学学报,2013,35(13):1384-1387.

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更新日期/Last Update: 2013-07-01