[1]龚丽莎,房兆飞,胡厚源,等.融合蛋白TAP-SSL5对血小板与粒细胞结合的影响[J].第三军医大学学报,2013,35(08):754-758.
 Gong Lisha,Fang Zhaofei,Hu Houyuan,et al.Effect of TAP-SSL5 fusion protein on binding of human platelets to neutrophils[J].J Third Mil Med Univ,2013,35(08):754-758.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
35卷
期数:
2013年第08期
页码:
754-758
栏目:
论著
出版日期:
2013-04-30

文章信息/Info

Title:
Effect of TAP-SSL5 fusion protein on binding of human platelets to neutrophils
作者:
龚丽莎房兆飞胡厚源刘成海孟璟
第三军医大学西南医院心血管内科,重庆市介入心脏病学研究所
Author(s):
Gong Lisha Fang Zhaofei Hu Houyuan Liu Chenghai Meng Jing
Department of Cardiology, Southwest Hospital, Third Military Medical University, Chongqing, 400038, China
关键词:
金黄色葡萄球菌超抗原样蛋白-5蜱抗凝血肽融合蛋白血小板中性粒细胞P-选择素糖蛋白配体-1
Keywords:
staphylococcal superantigen like protein-5 tick anticoagulant peptide fusion protein platelet neutrophil P-selectin glycoprotein ligand-1
分类号:
R331.143;R392.12;R392.3
文献标志码:
A
摘要:
目的   探讨融合蛋白TAP-SSL5对血小板与粒细胞结合作用的影响。   方法   采用CCK-8试剂盒检测TAP-SSL5对细胞活力的影响;流式细胞仪检测THP-1细胞表面CD162(PSGL-1)的表达,及TAP-SSL5对小鼠抗人CD162单抗KPL-1与THP-1细胞结合的抑制作用。以20 μmol/L ADP激活人血小板,采用流式细胞仪、瑞氏-姬姆萨染色检测血小板与THP-1细胞或人中性粒细胞的结合情况,并研究TAP-SSL5的干预作用。   结果   TAP-SSL5浓度在30 mg/L或以下时对THP-1细胞或中性粒细胞的活力无明显影响。流式细胞仪检测显示,TAP-SSL5(终浓度10 mg/L)能显著抑制KPL-1与THP-1细胞的结合;20 μmol/L ADP激活的血小板与THP-1细胞或中性粒细胞的结合率分别为(31.3±8.4)%和(35.6±5.9)%;细胞经10 mg/L TAP-SSL5预先处理后,其结合率分别降至(13.4±6.7)%和(10.4±6.4)% (P<0.01)。瑞氏-姬姆萨染色的结果表明,TAP-SSL5和SSL5均可抑制激活的血小板与中性粒细胞的结合(P<0.05)。   结论   TAP-SSL5可通过与PSGL-1的结合,而直接抑制激活的血小板与粒细胞的结合。
Abstract:
Objective   To investigate the effect of anti-inflammatory and anticoagulant tick anticoagulant peptide (TAP)-staphylococcal superantigen like protein-5 (SSL5) fusion protein on the binding of human platelets to neutrophils.    Methods   Cell viability was assessed by colorimetric cell counting kit-8 (CCK-8). Flow cytometry (FCM) was applied to detect the expression of CD162 (PSGL-1) on THP-1 cells as well as the inhibitory effect of TAP-SSL5 on the binding of mouse anti-human CD162 monoclonal antibody (KPL-1) to THP-1 cells. Platelets were activated by 20 μmol/L of ADP, and the binding rates of the activated platelets to THP-1 cells and neutrophils were assayed by FCM and Wright-Giemsa staining, respectively.    Results   TAP-SSL5≤30 mg/L did not affect the viability of THP-1 cells or human neutrophils, and the TAP-SSL5 at 10 mg/L competitively inhibited KPL-1 binding to THP-1 cells. The binding rates of activated platelets (by 20 μmol/L ADP) to THP-1 cells or neutrophils were (31.3±8.4)% and (35.6±5.9)%, respectively, which decreased to (13.4±6.7)% and (10.4±6.4)% after the THP-1 cells and neutrophils were pre-incubated with 10 mg/L TAP-SSL5 (P<0.01). The results of Wright-Giemsa staining showed that both TAP-SSL5 and SSL5 could inhibit the binding of activated platelets to neutrophils (P<0.05).    Conclusion   TAP-SSL5 can directly inhibit the platelet-neutrophil binding.

参考文献/References:

龚丽莎, 房兆飞, 胡厚源, 等. 融合蛋白TAP-SSL5对血小板与粒细胞结合的影响[J].第三军医大学学报,2013,35(8):754-758.

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更新日期/Last Update: 2013-04-22