[1]程航,曾方银,胡启文,等.广州地区耐甲氧西林金黄色葡萄球菌的分子分型及耐药性分析[J].第三军医大学学报,2013,35(08):696-701.
 Cheng Hang,Zeng Fangyin,Hu Qiwen,et al.Molecular typing and antimicrobial susceptibility of methicillin-resistant Staphylococcus aureus isolated from Guangzhou, China[J].J Third Mil Med Univ,2013,35(08):696-701.
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广州地区耐甲氧西林金黄色葡萄球菌的分子分型及耐药性分析(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
35卷
期数:
2013年第08期
页码:
696-701
栏目:
论著
出版日期:
2013-04-30

文章信息/Info

Title:
Molecular typing and antimicrobial susceptibility of methicillin-resistant Staphylococcus aureus isolated from Guangzhou, China
作者:
程航曾方银胡启文袁文常周人杰张霞袁吉振尚伟龙杨杰胡珍朱军民饶贤才
第三军医大学基础医学部微生物学教研室,重庆市微生物工程实验室;南方医科大学南方医院检验科;第三军医大学新桥医院急诊科
Author(s):
Cheng Hang Zeng Fangyin Hu Qiwen Yuan Wenchang Zhou Renjie Zhang Xia Yuan Jizhen Shang Weilong Yang Jie Hu Zhen Zhu Junmin Rao Xiancai
Department of Microbiology,Laboratory of Microbiological Engineering, College of Basic Medical Sciences, Third Military Medical University, Chongqing, 400038;Department of Laboratory Medicine, Nanfang Hospital, Nanfang Medical University, Guangzhou, Guangdong Province, 510515;Department of Emergency, Xinqiao Hospital, Third Military Medical University, Chongqing, 400037, China
关键词:
耐甲氧西林金黄色葡萄球菌分子分型脉冲场凝胶电泳多位点序列分型耐药谱
Keywords:
methicillin-resistant Staphylococcus aureus molecular typing pulsed-field gel electrophoresis multilocus sequence typing drug resistant profile
分类号:
R181.34;R372;R378.11
文献标志码:
A
摘要:
目的  了解广州地区耐甲氧西林金黄色葡萄球菌(methicillin-resistant Staphylococcus aureus,MRSA)分子型别及其对临床常用抗生素的耐药情况,为防控MRSA感染提供依据。   方法   收集2010年10月至2011年4月广州地区两家教学医院临床分离的MRSA 142株,应用SCCmecspa、多位点序列分型(multilocus sequence typing,MLST)、脉冲场凝胶电泳(pulsed-field gel electrophoresis,PFGE)等方法进行分子分型,检测这些菌株对苯唑西林、克林霉素等17种临床常用抗生素的药物敏感性,分析临床MRSA的耐药情况。   结果   142株MRSA中121株为医院获得型金葡菌(HA-MRSA),20株为社区获得型金葡菌(CA-MRSA),1株未定型MRSA。这些MRSA可分为16种spa型,8种ST型及13种PFGE型。HA-MRSA中的ST239-MRSA-Ⅲ-t030、ST239-MRSA-Ⅲ-t037和ST5-MRSA-Ⅱ-t002为主要流行克隆,也发现CA-MRSA 中ST59-MRSA-Ⅳ-t437克隆的流行,且这些流行克隆有特定的耐药谱。   结论   广州地区流行的金葡菌仍以HA-MRSA为主,同时也有一定量的CA-MRSA检出和传播,且CA-MRSA的多重药物敏感性已在下降。
Abstract:
Objective   To determine the molecular types and drug susceptibilities of methicillin-resistant Staphylococcus aureus (MRSA) prevailed in Guangzhou, China.   Methods   A total of 142 MRSA isolates were collected between October 2010 and April 2011 from two teaching hospitals in Guangzhou. The molecular typing methods, such as staphylococcal cassette chromosome mec (SCCmec) typing, staphylococcal protein A (spa) typing, multilocus sequence typing (MLST), and pulsed-field gel electrophoresis (PFGE), were applied for all MRSA strains, and the drug susceptibilities to 17 kinds of antibiotics were also determined.   Results   In the 142 MRSA isolates, there were 121 hospital-associated isolates (HA-MRSA), 20 community-associated isolates (CA-MRSA), and 1 non typeable MRSA. Sixteen spa types, eight sequence types (ST), and thirteen PFGE types were found. The major predominant clones were ST239-MRSA-Ⅲ-t030, ST239-MRSA-Ⅲ-t037 and ST5-MRSA-Ⅱ-t002 that belong to HA-MRSA. Furthermore, colne ST59-MRSA-Ⅳ-t437 that belongs to CA-MRSA was also found to be prevailed in Guangzhou. The major prevailed clones with specific drug resistant profiles were found.   Conclusion   HA-MRSA is still the most predominant MRSA in Guangzhou, and CA-MRSA with decreasing drug susceptibility is also found. It is the time to monitor the prevalence of CA-MRSA and drug resistance of MRSA.

参考文献/References:

程航, 曾方银, 胡启文, 等. 广州地区耐甲氧西林金黄色葡萄球菌的分子分型及耐药性分析[J].第三军医大学学报,2013,35(8):696-701.

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更新日期/Last Update: 2013-04-19