[1]张光伟,贺学农,周昌龙,等.血小板源性生长因子受体在实验性蛛网膜下腔出血后早期脑损伤中的作用[J].第三军医大学学报,2013,35(13):1398-1401.
 Zhang Guangwei,He Xuenong,Zhou Changlong,et al.Role of platelet-derived growth factor receptor in early brain injury following subarachnoid hemorrhage[J].J Third Mil Med Univ,2013,35(13):1398-1401.
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血小板源性生长因子受体在实验性蛛网膜下腔出血后早期脑损伤中的作用(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
35卷
期数:
2013年第13期
页码:
1398-1401
栏目:
论著
出版日期:
2013-07-15

文章信息/Info

Title:
Role of platelet-derived growth factor receptor in early brain injury following subarachnoid hemorrhage
作者:
张光伟贺学农周昌龙夏小辉孙晓川谢延风
重庆医科大学附属永川医院神经外科;重庆医科大学附属第一医院神经外科
Author(s):
Zhang Guangwei He Xuenong Zhou Changlong Xia Xiaohui Sun Xiaochuan Xie Yanfeng
Department of Neurosurgery,  Yongchuan Affiliated Hospital, Chongqing Medical University, Chongqing, 402160,Department of Neurosurgery,  First Affiliated Hospital, Chongqing Medical University, Chongqing, 400016, China
关键词:
蛛网膜下腔出血早期脑损伤血小板源性生长因子受体凋亡脑水肿
Keywords:
aneurysmal subarachnoid hemorrhage early brain injury platelet-derived growth factor receptor apoptosis cephaledema
分类号:
R341; R363; R743.35
文献标志码:
A
摘要:
目的      通过研究大鼠蛛网膜下腔出血(subarachnoid hemorrhage,SAH)后脑水肿及细胞凋亡情况,初步探讨血小板源性生长因子受体(platelet-derived growth factor receptor,PDGFR)参与早期脑损伤的可能机制。      方法      采用随机数字表法将成年雄性SD大鼠分配至假手术组、SAH组、SAH+Imatinib组、SAH+PDGF-BB组。Imatinib为PDGFR拮抗剂,PDGF-BB为其激动剂,每组18只。采用血管内丝线穿刺颈内动脉分叉处的方法,建立SAH模型。SAH+Imatinib组大鼠在建模前1 h,行腹腔注射Imatinib处理;SAH+PDGF-BB组大鼠建模前1 h,行侧脑室注射PDGF-BB处理。处理后24 h,对各组动物的死亡率、神经功能学评分和脑水含量指标进行研究;RT-PCR、Western blot检测海马组织Caspase-3的表达。      结果      SAH+PDGF-BB组大鼠死亡率、脑组织含水量最高,神经行为功能学评分最低(P<0.01);SAH+Imatinib组与SAH组比较,大鼠死亡率、脑组织含水量有所下降,神经行为功能学评分有所提高(P<0.01);与其他组比较,SAH+PDGF-BB组大鼠海马组织Caspase-3 mRNA表达最高(P<0.01),SAH+Imatinib组与SAH组比较,海马组织Caspase-3 mRNA表达有所下降(P<0.01);Western blot检测趋势与RT-PCR检测结果一致。      结论        PDGFR参与SAH后早期脑损害,其拮抗剂Imatinib能在一定程度上减轻早期脑损伤。
Abstract:
Objective      To observe cephaledema and cell apoptosis in rats following subarachnoid hemorrhage (SAH), and to explore the mechanism of platelet-derived growth factor receptor (PDGFR) involving in the development of early brain injury.       Methods      Adult male SD rats were randomly divided into 4 groups including a sham operation group, a SAH group, a SAH+imatinib group, and a SAH+PDGF-BB group (n=18). SAH mouse models were established by using intravascular silk puncture of the internal carotid artery crotch. The rats of the SAH+imatinib group were given an intraperitoneal injection of imatinib at 1 h before modeling, while those of the SAH+PDGF-BB group were given an intracerebroventricular injection of PDGF-BB at 1 h before modeling. After treatment for 24 h, the mortality, water content in brain and neurological function score were determined. Caspase-3 expression in hippocampus was detected by RT-PCR and Western blotting.       Results      The mortality, brain water content and caspase-3 expression level were the highest in the SAH+PDGF-BB group, but the neurological function score was the lowest (P<0.01). The parameters were reduced when SAH rats were injected with imatinib (P<0.01).       Conclusion      PDGFR may participate in the pathogenesis of early brain injury following SAH, and its antagonist imatinib can reduce brain injury to a certain degree.

参考文献/References:

张光伟,贺学农,周昌龙,等. 血小板源性生长因子受体在实验性蛛网膜下腔出血后早期脑损伤中的作用[J].第三军医大学学报,2013,35(13):1398-1401.

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更新日期/Last Update: 2013-07-01