[1]祝冰晶,王宇亮,罗虎,等.CA916798基因通过PI3K/AKT通路参与肺癌顺铂耐药[J].第三军医大学学报,2013,35(07):618-621.
 Zhu Bingjing,Wang Yuliang,Luo Hu,et al.CA916798 gene is involved in cisplatin resistance in human lung cancer through PI3K/AKT pathway[J].J Third Mil Med Univ,2013,35(07):618-621.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
35卷
期数:
2013年第07期
页码:
618-621
栏目:
论著
出版日期:
2013-04-15

文章信息/Info

Title:
CA916798 gene is involved in cisplatin resistance in human lung cancer through PI3K/AKT pathway
作者:
祝冰晶王宇亮罗虎周向东
第三军医大学西南医院呼吸内科
Author(s):
Zhu Bingjing Wang Yuliang Luo Hu Zhou Xiangdong
Department of Respiratory Diseases, Southwest Hospital, Third Military Medical University, Chongqing, 400038, China
关键词:
CA916798耐药顺铂PI3K/AKT
Keywords:
CA916798 drug resistance cisplatin PI3K/AKT
分类号:
R73-361; R734.2; R979.11
文献标志码:
A
摘要:
目的      在前期体外研究的基础上,建立裸鼠移植瘤模型,观察阻断PI3K/AKT通路对肺腺癌细胞A549及多药耐药肺腺癌细胞A549/CDDP内CA916798基因mRNA表达的影响。      方法       建立A549、A549/CDDP裸鼠移植瘤模型,以LY294002作用A549组和A549/CDDP组后,比较各组移植瘤生长情况,HE染色观察组织结构变化,实时荧光定量PCR检测肿瘤细胞中CA916798基因mRNA 的表达水平。      结果      成功建立裸鼠移植瘤模型,以LY294002分别阻断A549、A549/CDDP组裸鼠移植瘤细胞的PI3K/AKT通路后,移植瘤体积明显缩小,CA916798基因的表达明显下调(P<0.05)。      结论      抑制PI3K/AKT通路能够明显抑制肺腺癌细胞A549及A549/CDDP的恶性增殖。
Abstract:
Objective      To determine the effect of blocking the PI3K/AKT pathway on the expression of CA916798 gene in nude mouse transplanted tumor of human lung adenocarcinoma cell line A549 and the multidrug-resistant cell line A549/CDDP.       Methods      A total of 20 nude mice were randomly and equally divided into 4 groups, that is, A549, A549/CDDP, A549+LY294002, and A549/CDDP+LY294002 groups. The animal model with A549 cells and A549/CDDP cells transplanted tumor was established in these corresponding nude mice. LY294002 of 25 mg/kg was treated in the mice of the 2 later groups. The size of tumor was measured, and morphology of the mass was observed after HE staining. Real-time fluorescent quantitative PCR was performed to detect CA916798 mRNA expression in the tumor.       Results      The model of transplanted tumor was established in nude mice  successfully. Blocking PI3K/AKT pathway with LY294002 resulted in significantly decreased expression of CA916798 at mRNA level (P<0.05), and reduced tumor volume.       Conclusion      Inhibition of PI3K/AKT pathway significantly inhibits the malignant proliferation in lung adenocarcinoma cell A549 and multidrug-resistant cell line A549/CDDP.

参考文献/References:

祝冰晶, 王宇亮, 罗虎, 等. CA916798基因通过PI3K/AKT通路参与肺癌顺铂耐药[J].第三军医大学学报,2013,35(7):618-621.

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更新日期/Last Update: 2013-04-07