[1]皮燕,张莉莉,胡子成,等.miR-145表达状态对大鼠高血压动脉内膜增生的影响[J].第三军医大学学报,2013,35(08):707-711.
 Pi Yan,Zhang Lili,Hu Zicheng,et al.Role of miR-145 expression in arterial intimal hyperplasia of hypertension in rats[J].J Third Mil Med Univ,2013,35(08):707-711.
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miR-145表达状态对大鼠高血压动脉内膜增生的影响(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
35卷
期数:
2013年第08期
页码:
707-711
栏目:
论著
出版日期:
2013-04-30

文章信息/Info

Title:
Role of miR-145 expression in arterial intimal hyperplasia of hypertension in rats
作者:
皮燕张莉莉胡子成黎炳护尹延伟高长越李敬诚
第三军医大学大坪医院野战外科研究所神经内科
Author(s):
Pi Yan Zhang Lili Hu Zicheng Li Binghu Yin Yanwei Gao Changyue Li Jingcheng
Department of Neurology, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing, 400042, China
关键词:
高血压血管平滑肌细胞内膜增生miR-145
Keywords:
hypertension vascular smooth muscle cell intimal hyperplasia miR-145
分类号:
R322.121;R394.2;R544.1
文献标志码:
A
摘要:
目的   观察微小RNA-145(microRNA-145,miR-145)在高血压血管内膜增生中的作用。   方法   常规HE染色观察自发性高血压大鼠(SHR)和对照大鼠(WKY)主动脉内膜增生情况,逆转录多聚酶链反应(RT-PCR)方法检测不同病程SHR大鼠主动脉中miR-145的表达水平,并应用miR-145前体premiR-145和抑制剂2’OMe-miR-145分别升高和抑制血管平滑肌细胞(smooth muscle cell, VSMC)中miR-145的表达水平,从分化标志蛋白α平滑肌肌动蛋白(α-SMA)表达水平、VSMC的增殖和迁移能力等方面检测VSMC表型转化情况。   结果   ①SHR大鼠主动脉内膜增生,内膜/中膜面积百分比(39.7±12.1)%显著高于WKY组(3.8±1.2)%(P=0.001),α-SMA染色阳性的VSMC是增生内膜的主要细胞成分;②与WKY相比,SHR大鼠主动脉来源的VSMC表型转化明显增多,表现为增殖和迁移能力升高,而α-SMA表达降低(P<0.01);③SHR大鼠主动脉中的miR-145水平在8周龄时开始下降(P=0.045 6),至18周龄时降至仅为WKY组的7.5%;④应用premiR-145可上调SHR-VSMC中α-SMA的表达(P=0.005 6)并促进VSMC增殖迁移过程(P<0.01),而2’OMe-miR-145可抑制VSMC中α-SMA的表达(P=0.023 2)和VSMC增殖迁移过程(P<0.01)。   结论   高血压时动脉中miR-145水平呈进行性下降,可能是引起VSMC表型转化并促进动脉内膜增生的一个重要机制。
Abstract:
Objective   To determine the effect of miR-145 on arterial intimal hyperplasia in hypertension.   Methods   Spontaneous hypertension rats (SHR) and Wistar-Kyoto (WKY) rats were used in this study. Aortic intimal hyperplasia was evaluated pathologically. The expression of miR-145 in aorta of SHR was detected by reverse transcription polymerase chain reaction (RT-PCR). The phenotypic modulation of vascular smooth muscle cells (VSMCs) was assessed by detecting the expression of α-smooth muscle actin (α-SMA) and the capability of proliferation and migration in the presence of miR-145 precursor, premiR-145, or inhibitor 2’OMe-miR-145.   Results   SHR aortas exhibited obvious neo-intima formation which was composed primarily of VSMCs, with the ratios of intima/media significant higher than WKY rats (P=0.001). SHR-derived VSMCs exhibited marked phenotypic modulation, however, the expression of α-SMA was lower (P<0.01). The expression of miR-145 in SHR aortas was declined at the 8th week (P=0.045 6) and reduced to 7.5% of that of WKY rats at the 18th week. Expression of α-SMA and proliferative and migratory capacities of VSMCs were respectively upregulated and downregulated by premiR-145 and 2’OMe-miR-145.   Conclusion   The expression of miR-145 decreases progressively during hypertension, which may at least partly contribute to the excessive VSMCs phenotypic modulation and thus arterial intimal hyperplasia.

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更新日期/Last Update: 2013-04-19