[1]李晓萍,张志坚,屈纪富.姜黄素对大鼠重症胰腺炎的干预效应研究[J].第三军医大学学报,2013,35(08):759-763.
 Li Xiaoping,Zhang Zhijian,Qu Jifu.Effects of curcumin intervention on chemokine ENA-78 expression in rat severe acute pancreatitis[J].J Third Mil Med Univ,2013,35(08):759-763.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
35卷
期数:
2013年第08期
页码:
759-763
栏目:
论著
出版日期:
2013-04-30

文章信息/Info

Title:
Effects of curcumin intervention on chemokine ENA-78 expression in rat severe acute pancreatitis
作者:
李晓萍张志坚屈纪富
第三军医大学西南医院急救部; 巴南区人民医院ICU
Author(s):
Li XiaopingZhang ZhijianQu Jifu
Department of Emergency,Southwest Hospital,Third Military Medical University,Chongqing, 400038; Intensive Care Unit, Banan District People’s Hospital, Chongqing, 401320, China
关键词:
重症急性胰腺炎姜黄素趋化因子ENA-78基因表达
Keywords:
severe acute pancreatitis curcumin epithelial neutrophil activating peptide 78gene expression
分类号:
R282.71;R576;R965
文献标志码:
A
摘要:
目的   探讨趋化因子ENA-78(epithelial neutrophil activating peptide 78,ENA-78)在重症急性胰腺炎(acute pancreatitis,SAP)发病过程中的作用及姜黄素(curcumin,CUR)的干预效应。   方法   54只SD大鼠按完全随机法分为SAP组、姜黄素治疗(SAP+CUR)组和对照组,用4%牛磺胆酸钠逆行胆胰管注射建立大鼠模型,SAP+CUR组在建立模型前2 h 腹腔内注射姜黄素溶液(200 mg/kg),余组注射同等剂量的DMSO溶液。用ELISA法测定3组大鼠不同时点血清中ENA-78水平的变化;Western blot和免疫组化法检测胰腺组织不同时点ENA-78蛋白的表达水平;病理组织切片检查各时点胰腺的病理变化。   结果   SAP组血清中ENA-78水平随时间的延长逐渐增强,3 h后各时点血清ENA-78水平均高于对照组及SAP+CUR组(P<0.01);SAP组及SAP+CUR组术后胰腺组织ENA-78蛋白表达随时间延长逐渐增加,术后各时间点表达均显著高于对照组,但同时间点比较SAP+CUR组表达明显低于SAP组(P<0.01);病理组织切片检查显示SAP+CUR组各时点与SAP组相比较,胰腺病理损伤显著减轻。   结论   姜黄素能有效干预SAP,其机制可能与抑制趋化因子ENA-78的表达有关。
Abstract:
Objective   To investigate the role of epithelial neutrophil activating peptide 78 (ENA-78) in the pathogenesis of severe acute pancreatitis (SAP) and effects of curcumin intervention on ENA-78 expression in SAP.    Methods   Fifty-four SD rats were randomized into three groups: a SAP group, a curcumin treatment group (SAP+CUR) and a control group. SAP model was induced by retrograde infusion of 4% sodium taurocholate into the bili-pancreatic duct in rats. Rats in the SAP+CUR group were injected with curcumin (200 mg/kg) through abdominal cavity before operation while those in the control and SAP groups were injected with DMSO. ENA-78 in the serum was detected by ELISA at various time points. The expression of ENA-78 in pancreatic tissues was detected by Western blotting and immunohistochemistry. The histopathological changes were observed at the same time.    Results   Serum level of ENA-78 in 3 h after operation in the SAP group was significantly higher than that in the control group and SAP+CUR group (P<0.01). Compared with the control group, the expression of ENA-78 in the pancreatic tissues of rats in the SAP group and SAP+CUR group increased significantly in a time-dependent manner. Compared with the SAP group, the expression of ENA-78 in the pancreatic tissues of rats in the SAP+CUR group was decreased (P<0.01). Pathological injury of pancreatic tissues was more obvious in the SAP group than in the SAP+CUR group.    Conclusion   Curcumin efficiently interferes SAP and may be related to inhibition of ENA-78 expression.

参考文献/References:

李晓萍, 张志坚, 屈纪富. 姜黄素对大鼠重症胰腺炎的干预效应研究[J].第三军医大学学报,2013,35(8):759-763.

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更新日期/Last Update: 2013-04-22