[1]黄磊,储卫华,袁继超,等.丙戊酸钠通过p21调控大鼠神经干细胞的增殖[J].第三军医大学学报,2013,35(06):487-490.
 Huang Lei,Chu Weihua,Yuan Jichao,et al.Sodium valproate inhibits proliferation in rat neural stem cells through p21 pathway[J].J Third Mil Med Univ,2013,35(06):487-490.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
35卷
期数:
2013年第06期
页码:
487-490
栏目:
论著
出版日期:
2013-03-30

文章信息/Info

Title:
Sodium valproate inhibits proliferation in rat neural stem cells through p21 pathway
作者:
黄磊储卫华袁继超赵明月陈图南蒋周阳林江凯冯华
第三军医大学西南医院神经外科,全军神经外科研究所,全军神经创伤防治重点实验室
Author(s):
Huang Lei Chu Weihua Yuan Jichao Zhao Mingyue Chen Tunan Jiang Zhouyang Lin Jiangkai Feng Hua
Institute of Neurosurgery, PLA Key Laboratory of Neurotrauma Prevention and Treatment,  Southwest Hospital, Third Military Medical University, Chongqing, 400038, China
关键词:
丙戊酸钠脊髓神经干细胞增殖 p21
Keywords:
sodium valproate spinal cord neural stem cells proliferation p21
分类号:
R322.8;R329.28;R971.6
文献标志码:
A
摘要:
目的      探讨丙戊酸钠(sodium valproate,VPA)对体外培养的成年雌大鼠脊髓神经干细胞(neural stem cells,NSCs)增殖的影响。      方法      采用不同浓度的VPA(10-5 、10-4 、10-3、10-2 、10-1、1、10 mmol/L)作用于NSCs,CCK-8法检测在不同时间点(0、24、48、72 h)对细胞增殖的影响;VPA(10-5、1 mmol/L)作用于NSCs 48 h后,流式细胞仪测定细胞周期分布,PCR测定p21在基因水平的表达,Western blot测定p21在蛋白质水平的表达。      结果      CCK-8检测显示,当VPA浓度>10-5mmol/L时,体外培养的成年大鼠脊髓NSCs的增殖受到明显抑制,且具有时间依赖性。流式细胞仪细胞周期检测显示,同样浓度下,VPA可阻滞NSCs由G0/G1期向S期转换,表现为G0/G1期细胞增多,S期细胞减少,G2/M期细胞减少。PCR检测发现VPA可促进p21基因水平的表达。Western blot检测发现VPA可促进p21蛋白质水平的表达,各组间差异均有统计学意义(P<0.05)。      结论      VPA可能通过促进p21表达,使NSCs阻滞于G0/G1期,最终抑制NSCs增殖。
Abstract:
Objective      To determine the effect of sodium valproate (VPA) on the proliferation and cell cycle in adult female rat spinal neural stem cells (NSCs).       Methods      Cell proliferation was assessed by CCK-8 assay after the cells were treated with VPA at different concentrations of 10-5, 10-4, 10-3, 10-2, 10-1, 1 or 10 mmol/L for 0, 24, 48 or 72 h. After NSCs were treated with VPA at a dose of 10-5 and 1 mmol/L for 48 h, cell cycle was analyzed by flow cytometry and the expression of p21(cyclin-dependent kinase inhibitor) was detected by PCR and Western blot analysis.       Results      CCK-8 staining colorimetry showed that the proliferation of NSCs was markedly inhibited in a time-dependent manner when the concentration of VPA was more than 10-5mmol/L. Flow cytometry indicated more cultured NSCs were arrested in the G0/G1 phase and fewer at the S phase after being treated with VPA, which indicated that VPA arrested the transition of NSCs from G0/G1 phase to S phase. PCR and Western blot analysis indicated that VPA enhanced the expression of p21 at mRNA and protein levels(P<0.05).       Conclusion      VPA may arrest NSCs at G0/G1 by increasing the expression of p21, and then finally inhibit the proliferation of NSCs.

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更新日期/Last Update: 2013-03-19