[1]黄翀,江旭品,张琼,等.低氧环境对创面修复主要效应细胞表达MMP-9的作用[J].陆军军医大学学报(原第三军医大学学报),2013,35(04):293-296.
 Huang Chong,Jiang Xupin,Zhang Qiong,et al.Hypoxia up-regulates MMP-9 in epidermal cells during wound repair[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(04):293-296.
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陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
35卷
期数:
2013年第04期
页码:
293-296
栏目:
论著
出版日期:
2013-02-28

文章信息/Info

Title:
Hypoxia up-regulates MMP-9 in epidermal cells during wound repair
作者:
黄翀江旭品张琼贾丽杰张家平黄跃生
第三军医大学西南医院全军烧伤研究所,创伤、烧伤与复合伤国家重点实验室
Author(s):
Huang Chong Jiang Xupin Zhang Qiong Jia Lijie Zhang Jiaping Huang Yuesheng
State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Burns, Southwest Hospital, Third Military Medical University, Chongqing, 400038, China
关键词:
创面愈合低氧HaCaT细胞人成纤维细胞MMP-9
Keywords:
wound healinghypoxia HaCaT cell lineshuman dermal fibroblastMMP-9
分类号:
R322.991;R329.25;R364.4
文献标志码:
A
摘要:
目的      探讨低氧(2%O2)对创面修复主要效应细胞(表皮细胞及真皮成纤维细胞)表达MMP-9的影响。      方法      常规培养人表皮细胞及人真皮成纤维细胞,分为常氧组和低氧组(设低氧培养3 h和6 h两个组),采用Western blot、明胶酶谱法和荧光定量PCR法检测人表皮细胞和真皮成纤维细胞MMP-9细胞蛋白表达、MMP-9细胞外分泌和MMP-9转录水平变化。      结果      常氧培养的表皮细胞中,MMP-9呈一定程度的基础表达和细胞外分泌。与常氧组比较,低氧能明显促进表皮细胞MMP-9的蛋白表达(6 h时增强约20%,P<0.01)和MMP-9的细胞外分泌[(44.03±3.00) vs (60.64±3.77),P<0.01]。常氧与低氧条件下真皮成纤维细胞极少表达和分泌MMP-9。荧光实时定量PCR结果显示,低氧培养条件下表皮细胞MMP-9 mRNA水平显著增高,常氧对照组为(1.67±0.69),低氧6 h组为(5.47±0.51),两者比较差异有统计学意义(P=0.002)。      结论      低氧刺激能显著诱导表皮细胞,而非真皮成纤维细胞,表达和分泌MMP-9,这一作用可能与低氧刺激促进MMP-9的转录有关。
Abstract:
Objective      To determine the effect of hypoxia (2% oxygen) on the expression of MMP-9 in main effector cells and epidermal cells and fibroblasts during wound repair.       Methods      Human epidermal HaCaT cells and primarily cultured fibroblasts from fresh foreskin tissues by circumcision were divided into 2 groups, normoxic group (synchronous culture, no treatment), and hypoxia group (including 2 subgroups with 3 and 6 h under hypoxia). The expressin of MMP-9 in epidermal cells and fibroblasts, extracellular secretion of MMP-9 precursor expressions and changes of MMP-9 at the transcription level were respectively by Western blotting, gelatin zymography and fluorescence quantitative PCR.       Results      MMP-9 had a certain degree of basal expression and extracellular secretion in epidermal cells from normoxic group. Hypoxia significantly promoted the expression levels of MMP-9 protein in the epidermal cells (20% increased in 6 h compared with the normoxic group, P<0.01), and the extracellular secretions of MMP-9 precursor protein in hypoxia group at 6 h and normoxic control group (60.64±3.77 vs 44.03±3.00, P<0.01). However, fibroblasts almost never expressed or secreted MMP-9 under normoxic and hypoxic conditions. Fluorescence real-time quantitative PCR indicated hypoxia increased MMP-9 mRNA expression in epidermal cells (hypoxia group at 6 h 5.47±0.51 vs normoxic control group 1.67±0.69, P=0.002).       Conclusion      Hypoxic stimulation significantly induces the expression and secretion of MMP-9 in epidermal cells, rather than dermal fibroblast, which may be related to the promotion of MMP-9 at transcription level in the wound under local hypoxic environment.

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更新日期/Last Update: 2013-02-25