[1]王恒,王勇,江晓霁,等.莪术醇通过JAK3/STAT信号途径抑制Jurkat细胞增殖[J].第三军医大学学报,2013,35(05):408-411.
 Wang Heng,Wang Yong,Jiang Xiaoji,et al.Proliferation-inhibiting effect of curcumol on human T-cell leukemia cell line Jurkat via blocking JAK3/STAT pathway[J].J Third Mil Med Univ,2013,35(05):408-411.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
35卷
期数:
2013年第05期
页码:
408-411
栏目:
论著
出版日期:
2013-03-15

文章信息/Info

Title:
Proliferation-inhibiting effect of curcumol on human T-cell leukemia cell line Jurkat via blocking JAK3/STAT pathway
作者:
王恒王勇江晓霁王志中刘晓飞方勇飞
第三军医大学西南医院中西医结合科;解放军第474医院康复科
Author(s):
Wang Heng Wang Yong Jiang Xiaoji Wang Zhizhong Liu Xiaofei Fang Yongfei
Department of Integrated Chinese and Western Medicine, Southwest Hospital, Third Military Medical University, Chongqing, 400038; Department of Rehabilitation, No.474 Hospital of PLA, Urumchi, Xinjiang Uygur Autonomous Region, 830011, China
关键词:
莪术醇Jurkat T细胞JAK3-STAT细胞增殖
Keywords:
curcumol Jurkat T cells JAK3-STAT cell proliferation
分类号:
R285.5; R329.28; R392.12
文献标志码:
A
摘要:
目的      观察莪术醇对抑制Jurkat细胞增殖的影响,并从JAK3/STAT信号途径探讨其作用机制。      方法        不同浓度(6.25、12.5、25、50 μg/mL)莪术醇干预处理人Jurkat细胞株,分别用新型四唑单钠盐法、流式细胞技术观察检测细胞增殖活力及细胞周期分布。Hoechst 33258染色,观察莪术醇诱导的细胞凋亡形态学变化。设正常对照组、IL-2刺激组、JAK3抑制剂组及莪术醇干预组,Western blot检测细胞中JAK3、STAT3及STAT5a磷酸化蛋白表达量的变化。      结果        不同浓度的莪术醇体外处理24 h后,Jurkat 细胞的增殖受到明显抑制,呈浓度依赖性下降(P<0.05)。细胞周期分布结果显示,随着莪术醇作用浓度的增加,细胞凋亡的比例明显升高,S期细胞比例呈浓度依赖性增多,G2/M细胞比例则呈减少趋势。50 μg/mL的莪术醇处理24 h后,细胞表现出核固缩、碎裂等典型的凋亡形态学变化。Western blot检测结果显示,50 μg/mL 的莪术醇可抑制JAK3与STAT5a磷酸化蛋白的表达(P<0.05, P<0.01),但对STAT3无明显影响(P>0.05)。      结论        莪术醇作用于Jurkat细胞S~G2/M期,阻滞S期细胞向G2/M期移行,并下调JAK3/STAT5信号分子以抑制该细胞增殖。
Abstract:
Objective        To observe the inhibitory effect of curcumol on Jurkat cells, and to explore the potential mechanism through JAK3 (Janus kinase 3)/STAT (signal transducers and activators of transcription) signal pathway.       Methods        Cell viability was assessed by tetrazolium single sodium salt, and the characteristics of curcumol-induced cell cycle arrest was detected by flow cytometry (FCM) after Jurkat cells exposed to different concentrations of curcumol (6.25, 12.5, 25 and 50 μg/mL) for 24 h. The cell morphology of apoptosis induced by curcumol was assessed by Hoechst 33258 staining. The Jurkat cells were divided into four groups including a normal control group, a interleukin-2 (IL-2) stimulation group, a JAK3 inhibitor group and a curcumol intervention group, and the protein expression levels of p-JAK3 (phosphorylated JAK3), p-STAT3 and p-STAT5a were determined by Western blot analysis.       Results        After different concentrations of curcumol treatment, the Jurkat cell viability decreased significantly in a dose-dependent manner (P<0.05). FCM results showed that apoptotic rate and the proportion of the cells arrested in S phase were elevated along with curcumol concentration increase, while the proportion of cells in G2/M phase declined gradually. After exposure to curcumol for 24 h, the Jurkat cells underwent typical apoptotic morphological changes, such as nucleus condensation and fragmentation. The expression levels of p-JAK3 and p-STAT5a in Jurkat cells were suppressed by 50 μg/mL of curcumol (P<0.05, P<0.01), while there was no obvious change in STAT3 expression (P>0.05).       Conclusion        The underlying mechanism of curcumol on suppressing the proliferation of Jurkat cells may be associated with S to G2/M phase arresting and JAK3/STAT5 pathway down-regulation.

参考文献/References:

王恒, 王勇, 江晓霁, 等. 莪术醇通过JAK3/STAT信号途径抑制Jurkat细胞增殖[J].第三军医大学学报,2013,35(5):408-411.

更新日期/Last Update: 2013-03-05