[1]邬桂玉,赵敏,计岩.溴芬酸钠/壳聚糖纳米粒蛋白胶胶联羊膜的制备及其对兔角膜新生血管的影响[J].第三军医大学学报,2013,35(06):495-499.
 Wu Guiyu,Zhao Min,Ji Yan.Preparation of bromfenac sodium/chitosan nanoparticles loaded fibrin-binding amniotic membrane and its effect on rabbit corneal neovascularization in vitro[J].J Third Mil Med Univ,2013,35(06):495-499.
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溴芬酸钠/壳聚糖纳米粒蛋白胶胶联羊膜的制备及其对兔角膜新生血管的影响(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
35卷
期数:
2013年第06期
页码:
495-499
栏目:
论著
出版日期:
2013-03-30

文章信息/Info

Title:
Preparation of bromfenac sodium/chitosan nanoparticles loaded fibrin-binding amniotic membrane and its effect on rabbit corneal neovascularization in vitro
作者:
邬桂玉赵敏计岩
重庆医科大学附属第一医院眼科,重庆市眼科学重点实验室
Author(s):
Wu Guiyu Zhao Min Ji Yan
Department of Ophthalmology, Chongqing Key Laboratory of Ophthalmology, First Affiliated Hospital, Chongqing Medical University, Chongqing, 400016, China
关键词:
溴芬酸钠纤维蛋白胶壳聚糖纳米粒羊膜角膜新生血管
Keywords:
bromfenac sodium fibrin glue chitosan nanoparticle amniotic membrane corneal neovascularization
分类号:
R318.08;R772.2;R944.9
文献标志码:
A
摘要:
目的      制备一种具有双重缓释功能的新型生物复合材料溴芬酸钠/壳聚糖纳米粒胶联羊膜(BF/CS-FBAM),初步研究其体外缓释性能及对角膜新生血管的影响。      方法      采用离子胶联法制备溴芬酸钠/壳聚糖纳米粒并观察其表征,将载药纳米粒混入纤维蛋白胶中,再将混合物喷涂到羊膜表面制成BF/CS-FBAM,并进行形态学和体外释药观察,最后通过兔角膜碱烧伤模型观察BF/CS-FBAM对角膜新生血管的影响,用实时荧光定量PCR方法检测术后角膜中VEGF的表达。      结果      溴芬酸钠/壳聚糖纳米粒呈球形,表面光滑,大小均一,其粒径为(548.7±14.4)nm,Zeta电位为(29.8±2.2) mV,包封率为(67.11±4.56)%,载药量为(57.71±5.38)%。载有纳米药的纤维蛋白胶与羊膜黏合紧密,表面呈网状结构,纳米药充斥其中。在体外,BF/CS-FBAM的释药可达14 d。在碱烧伤模型中,术后BF/CS-FBAM组和BF/CS组在减少炎症反应、抑制新生血管及降低VEGF表达方面都显著优于对照组,且各时间点的差异有统计学意义(P<0.05);同时,术后14 d和21 d BF/CS-FBAM组中VEGF的表达明显低于BF/CS组(P<0.05)。      结论      制备的BF/CS-FBAM作为一种新型生物移植材料可在局部缓慢释放溴芬酸钠,并对角膜新生血管的产生有较好的抑制性。
Abstract:
Objective      To prepare a novel biological composite material, bromfenac sodium/chitosan nanoparticle loaded fibrin-binding amniotic membrane (BF/CS-FBAM), with double sustained-released function, and investigate the drug release in vitro and its effect on rabbit corneal neovascularization (CNV).       Methods      The ionotropic gelation was used to produce bromfenac sodium/chitosan nanoparticles (BF/CS). After its physic properties were identified, the BF/CS nanoparticles were mixed with fibrin glue (FG), and then sprayed to the surface of amniotic membrane (AM). After BF/CS-FBAM was obtained, the morphological changes and drug release in vitro were observed and measured. The effect of BF/CS-FBAM for CNV was observed in rabbit corneal alkali burn model, meanwhile the expression of vascular endothelial growth factor (VEGF) in the cornea were quantified by reverse transcription-quantitative PCR (RT-qPCR).       Results      The nanoparticles were smooth and spherical, in a diameter of 548.7±14.4 nm, and had a positive zeta potential of 29.8±2.2 mV. The encapsulation efficiency and loading capacity of BF/CS nanoparticles were (67.11±4.56)% and (57.71±5.38)% respectively. The nanoparticle-loaded fibrin gel was firmly adhered to the AM. Moreover, nanoparticles were coated within FBAM symmetrically, and its surface had a netted structure. The BF released from the BF/CS-FBAM was sustained for approximately 14 d in vitro. In rabbit corneal alkali burn model, BF/CS-FBAM group and BF/CS group had better curative effect in attenuating inflammatory reaction, anti-CNV and down-regulating VEGF than control group in every time point after injury (P<0.05). Meanwhile, the expression of VEGF in BF/CS-FBAM group was significantly lower than BF/CS group in the 14th and 21st day after injury (P<0.05).       Conclusion      As a novel biological implantable material, the fabricated BF/CS-FBAM locally releases BF in a sustained way and inhibits the generation of CNV.

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更新日期/Last Update: 2013-03-19