[1]臧春宝,曹姝,李芳,等.IL-6对食管癌细胞侵袭迁移及上皮间质转化的影响[J].第三军医大学学报,2013,35(04):302-306.
 Zang Chunbao,Cao Shu,Li Fang,et al.Effects of interleukin-6 on invasion, migration and epithelial-mesenchymal transition in human esophageal carcinoma cells[J].J Third Mil Med Univ,2013,35(04):302-306.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
35卷
期数:
2013年第04期
页码:
302-306
栏目:
论著
出版日期:
2013-02-28

文章信息/Info

Title:
Effects of interleukin-6 on invasion, migration and epithelial-mesenchymal transition in human esophageal carcinoma cells
作者:
臧春宝曹姝李芳陈兴月李少林
重庆医科大学基础医学院放射医学教研室
Author(s):
Zang Chunbao Cao Shu Li Fang Chen Xingyue Li Shaolin
Department of Radiation Medicine, Chongqing Medical University, Chongqing, 400016, China
关键词:
IL-6食管癌Stat3上皮间质转化侵袭迁移
Keywords:
interleukin-6esophageal carcinomaStat3epithelial-mesenchymal transitioninvasion/migration
分类号:
R392.11;R730.23;R735.1
文献标志码:
A
摘要:
目的      研究白细胞介素-6(interleukin-6, IL-6)对食管癌Eca-109细胞增殖、侵袭迁移及上皮间质转化的影响,探讨其作用机制。      方法      用不同浓度(0、25、50和100 ng/mL)的IL-6处理Eca-109细胞,采用MTT法检测细胞增殖,细胞划痕实验、Transwell小室实验检测细胞迁移侵袭能力,荧光定量PCR检测E-cadherin、N-cadherin、Vimentin和Twist1 mRNA的表达,Western blot检测Stat3、p-Stat3、E-cadherin、N-cadherin、Vimentin和Twist1蛋白表达。      结果      与对照组(0 ng/mL)比较,IL-6处理组(25、50、100 ng/mL)在IL-6作用后第1~5天 Eca-109细胞增殖增加,呈量效-时效关系(P<0.05,P<0.01)。IL-6各浓度(0、25、50 ng/mL)组24 h细胞迁移距离分别为(18.20±0.89)、(23.77±0.40)和(25.27±0.93) mm,与对照组(0 ng/mL)比较,IL-6处理组(25、50 ng/mL) Eca-109细胞体外迁移距离明显增加(P<0.05, P<0.05)。IL-6各浓度(0、25、50 ng/mL)组穿过Matrigel胶的细胞分别为(70.33±2.36)、(103.00±3.51)、(118.00±4.00)个/视野,与对照组(0 ng/mL)比较,IL-6处理组(25、50 ng/mL) Eca-109细胞体外穿过Matrigel胶的细胞数量明显增加(P<0.05, P<0.05)。荧光定量PCR结果显示,与对照组(0 ng/mL)相比,IL-6处理(25、50 ng/mL)组N-cadherin、Vimentin和Twist1 mRNA达增加(P<0.05, P<0.05),E-cadherin mRNA表达降低(P<0.05)。Western blot结果显示,与对照组(0 ng/mL)相比,IL-6各处理(25、50 ng/mL)组p-Stat3、N-cadherin、Vimentin和Twist1蛋白表达增加,E-cadherin蛋白表达降低,Stat3蛋白表达没有明显改变。      结论      IL-6可提高食管癌Eca-109细胞体外增殖、侵袭、迁移能力,促进上皮间质转化过程,其机制可能通过活化Stat3蛋白,上调Twist1的表达,进一步调控EMT蛋白(E-cadherin、N-cadherin和Vimentin)的表达。
Abstract:
Objective      To determine the effects of interleukin-6 (IL-6) on the cell proliferation, invasion, migration and epithelial-mesenchymal transition in human esophageal carcinoma cells.       Methods      The human esophageal carcinoma Eca-109 cells were treated with IL-6 at a dose of 0, 25, 50 and 100 ng/mL, cell proliferation, migration and invasion were detected by MTT assay, cell scratch test and Transwell chamber assay respectively. The mRNA expression of E-cadherin, N-cadherin, Vimentin and Twist1 were evaluated by real-time quantitative PCR. The protein expression levels of Stat3, p-Stat3, E-cadherin, N-cadherin, Vimentin and Twist1 were detected by Western blotting.       Results      Compared with control group (0 ng/mL IL-6 treatment), the cell proliferation was in a dose- and time-dependent manner when the Eca-109 cells were treated by IL-6 at 25, 50 and 100 ng/mL for 1 to 5 d (P<0.05, 0.01). IL-6 at 0, 25 and 50 ng/mL resulted in cell 24-hour migration distance of 18.20±0.89, 23.77±0.40 and 25.27±0.93 mm respectively, with the 2 later doses significantly longer than the former (P<0.05). IL-6 of above doses also resulted in the number of 24-hour Matrigel-permeating Eca-109 cells of 70.33±2.36, 103.00±3.51 and 118.00±4.00 /HP, respectively, with the number in the 2 later groups significantly larger than in the former group (P<0.05). Real time RT-PCR showed that the mRNA expression levels of N-cadherin, Vimentin and Twist1 were significantly increased and the mRNA expression level of E-cadherin was decreased in IL-6 treatment groups (25 and 50 ng/mL), significantly different from those in 0 ng/mL group (P<0.05). Western blotting showed that the protein levels of p-Stat3, N-cadherin, Vimentin and Twist1 were significantly increased, and that of E-cadherin was decreased, while that of Stat3 had no change in IL-6 treatment groups (25 and 50 ng/mL) when compared with 0 ng/mL group (P<0.05).       Conclusion      IL-6 promotes the cell proliferation, invasion, migration and epithelial mesenchymal transition in human esophageal carcinoma cells by constitutively activating Stat3, up-regulating Twist1, and regulating EMT proteins (E-cadherin, N-cadherin and Vimentin).

参考文献/References:

臧春宝, 曹姝, 李芳, 等. IL-6对食管癌细胞侵袭迁移及上皮间质转化的影响[J].第三军医大学学报,2013,35(4):302-306.

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更新日期/Last Update: 2013-02-25