[1]王杨,陈彬,姜晓梅,等.睾酮对乳腺癌细胞中FEN1表达的影响[J].第三军医大学学报,2013,35(02):95-98.
 Wang Yang,Chen Bin,Jiang Xiaomei,et al.Effect of testosterone on FEN1 expression in breast cancer cells[J].J Third Mil Med Univ,2013,35(02):95-98.
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睾酮对乳腺癌细胞中FEN1表达的影响(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
35卷
期数:
2013年第02期
页码:
95-98
栏目:
论著
出版日期:
2013-01-30

文章信息/Info

Title:
Effect of testosterone on FEN1 expression in breast cancer cells
作者:
王杨陈彬姜晓梅王越付晓红何凤田
第三军医大学基础医学部生物化学与分子生物学教研室
Author(s):
Wang Yang Chen Bin Jiang Xiaomei Wang Yue Fu Xiaohong He Fengtian
Department of Biochemistry and Molecular Biology, College of Basic Medical Sciences, Third Military Medical University, Chongqing, 400038, China
关键词:
乳腺肿瘤细胞系 肿瘤睾酮DNA瓣核酸内切酶类
Keywords:
breast neoplasmscell line tumortestosteroneflap endonucleases
分类号:
R737.9;R968.1;R977.12
文献标志码:
A
摘要:
目的      探讨睾酮(testosterone,T)在乳腺癌细胞中对瓣状核酸内切酶(flap endonuclease 1,FEN1)表达的影响及其机制。      方法      以MCF-7作为研究对象,采用RT-PCR观察雌二醇(17β-estradiol,E2)单独作用以及分别与雌激素受体(estrogen receptor,ER)拮抗剂ICI182,780(ICI)和MAPK途径抑制剂U0126共同作用时FEN1表达的变化。以MCF-7aro(芳香化酶过表达的MCF-7)作为研究对象,采用RT-PCR观察加入单独的睾酮以及睾酮和芳香化酶抑制剂来曲唑(letrozole)共同作用时FEN1表达的变化;采用Western blot观察睾酮单独作用以及分别与来曲唑和U0126共同作用时FEN1、p-ERK和p-Elk的变化。      结果      与对照组比较,加入雌二醇后MCF-7细胞中FEN1 mRNA的表达升高2.04倍(P<0.01),加入ICI和U0126后分别降低10.63倍和2.17倍(P<0.01)。加入睾酮后MCF-7aro细胞中FEN1 mRNA的表达升高1.66倍(P<0.01),蛋白的表达升高1.80倍(P<0.01);加入来曲唑后FEN1 mRNA的表达下降2.38倍,蛋白的表达降低1.84倍,加入U0126后蛋白的表达降低2.28倍(P<0.01);加入睾酮后ERK和Elk-1的磷酸化水平分别升高2.28倍和2.60倍(P<0.01),加入来曲唑后ERK和Elk-1的磷酸化水平分别降低2.60倍和2.37倍(P<0.01),加入U0126后ERK和Elk-1的磷酸化水平分别降低10.38倍和119.50倍(P<0.01)。      结论      睾酮可通过MAPK途径上调MCF-7 aro细胞中FEN1的表达。
Abstract:
Objective      To determine the effect of testosterone on the expression of flap endonuclease 1 (FEN1) in breast cancer.       Methods      The expression of FEN1 in MCF-7 cells was observed by RT-PCR after the cells were treated with 17β-estradiol (E2), E2 combined with ICI182, 780, an estrogen receptor antagonist, and E2 combined with U0126, a MEK inhibitor, respectively. In MCF-7 cells over-expressing aromatase (MCF-7/Aro), the expression of FEN1 was observed by RT-PCR when cells were treated with testerone or testerone combined with letrozole, one of aromatase inhibitors. The expression of FEN1 protein, p-ERK and p-Elk was observed by Western blotting when the cells were treated with testosterone, testosterone combined with letrozole, or combined with U0126.       Results      In MCF-7 cells, E2 resulted in an increase in the expression of FEN1 mRNA by 2.04 folds (P<0.01), which was inhibited by ICI182,780 or U0126 (10.63 and 2.17 folds, P<0.01). In MCF-7aro cells, testosterone resulted in an increase in the expression of FEN1 at mRNA and protein levels by 1.66 and 1.80 folds respectively(P<0.01), which was inhibited by letrozole (2.38 and 1.84 folds, P<0.01) or U0126(2.28 folds for protein only, P<0.01). Testosterone increased the phosphorylation of p-ERK and p-Elk by 2.28 and 2.60 folds, which was inhibited by letrozole by 10.38 folds or U0126 by 119.50 folds(P<0.01).       Conclusion      Testosterone up-regulates FEN1 in MCF-7aro cells through MAPK signaling pathway.

参考文献/References:

王杨, 陈彬, 姜晓梅, 等. 睾酮对乳腺癌细胞中FEN1表达的影响[J].第三军医大学学报,2013,35(2):95-98.

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更新日期/Last Update: 2013-01-18