[1]杨林,吴小候,罗春丽,等.肾癌细胞来源的exosomes诱导Jurkat T细胞凋亡[J].第三军医大学学报,2013,35(05):426-430.
 Yang Lin,Wu Xiaohou,Luo Chunli,et al.Exosomes derived from renal cancer cells induce Jurkat T cell apoptosis in vitro[J].J Third Mil Med Univ,2013,35(05):426-430.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
35卷
期数:
2013年第05期
页码:
426-430
栏目:
论著
出版日期:
2013-03-15

文章信息/Info

Title:
Exosomes derived from renal cancer cells induce Jurkat T cell apoptosis in vitro
作者:
杨林吴小候罗春丽王丹陈力学
重庆医科大学:附属第一医院泌尿外科,检验医学院实验诊断教研室,附属第一医院实验研究中心
Author(s):
Yang Lin Wu Xiaohou Luo Chunli Wang Dan Chen Lixue
Department of Urology,Experimental Research Center,First Affiliated Hospital; Department of Laboratory Diagnosis, College of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, China
关键词:
肾癌exosomes免疫逃逸凋亡
Keywords:
renal cancerexosomesimmune escapeapoptosis
分类号:
R730.23; R730.3; R737.11
文献标志码:
A
摘要:
目的      体外研究肾癌786-0细胞来源的exosomes介导肿瘤免疫逃逸的机制。      方法      采用CCK-8法检测肾癌786-0细胞来源的exosomes对Jurkat T细胞生长的影响,瑞氏-姬姆萨染色检测Jurkat T细胞形态变化,Annexin V-FITC/PI双染色流式细胞术检测Jurkat T细胞凋亡率,ELISA法检测Jurkat T细胞分泌功能,可溶性Fas阻断实验检测exosomes对Jurkat T细胞凋亡率的影响,Western blot检测exosomes中FasL、及Jurkat T细胞caspase、Bax及Bcl-2蛋白的表达。      结果      肾癌786-0细胞来源的exosomes可抑制Jurkat T细胞生长,10 μg/mL exosomes作用于Jurkat T细胞24 h,生长抑制率为(19.64±0.92)%,72 h为(36.24±1.12)%;400 μg/mL exosomes作用24 h,生长抑制率为(55.96±1.35)%,72 h为(76.51±1.37)%。Exosomes诱导Jurkat T细胞凋亡,10 μg/mL exosomes作用于Jurkat T细胞8 h,凋亡率为(7.31±1.32)%,24 h为(20.19±1.47)%;400 μg/mL exosomes作用8 h,凋亡率为(27.28±1.29)%,24 h为(41.72±0.88)%。Exosomes还明显抑制Jurkat T细胞IL-2、IFN-γ、IL-6、IL-10的分泌水平;exosomes高表达FasL,可溶性Fas阻断实验能逆转Jurkat T细胞的凋亡;凋亡诱导过程中caspase-3、caspase-8、caspase-9被激活,Bax/Bcl-2上调。      结论      肾癌786-0细胞分泌的exosomes能诱导Jurkat T细胞凋亡,介导肿瘤免疫逃逸。
Abstract:
Objective      To investigate the underlying mechanism of exosomes derived from renal cancer cell lines 786-0 to mediate tumor immune escape in vitro.       Methods      CCK-8 assay was used to determine the effects of exosomes on proliferation in Jurkat T cells. Morphological changes were  by wright-giemsa staining;flow cytometry with Annexin V-FITC/PI double staining was used to detect the apoptosis;secretion functions of Jurkat T cell were detected by ELISA assay; effects of exosomes on apoptosis of Jurkat T cell were detected by soluble Fas block experiment; effects on the protein expression of FasL, caspase, Bax and Bcl-2 were assessed by Western blot analysis.       Results      Exosomes could inhibit Jurkat T cell proliferation, 10 μg/mL exosomes act on Jurkat T cell for 24 and 72 h, growth inhibition rate was (19.64±0.92)% and (36.24±1.12)%; while 400 μg/mL exosomes act on it for 24 h and 72 h, growth inhibition rate was (55.96±1.35)% and (76.51±1.37)% respectively. Exosomes could induce Jurkat T cell apoptosis, 10 μg/mL exosomes act on Jurkat T cell for 8 h, apoptosis rate was (7.31±1.32)%, extending this monitoring to 24 h, apoptosis rate was (20.19±1.47)%; while 400 μg/mL exosomes act on it for 8 and 24 h, apoptosis rate was (27.28±1.29)% and (41.72±0.88)% respectively. Exosomes also suppressed IL-2, IFN-γ, IL-6 and IL-10 secretion obviously. FasL was highly expressed in exosomes, soluble Fas block could reverse Jurkat T cell apoptosis. In this course, caspase-3, caspase-8, caspase-9 were activated, and the ratio of Bax/Bcl-2 increased.       Conclusion      Exosomes could inhibit the growth of Jurkat T cell and induce apoptosis.It could mediate tumor immune escape.

参考文献/References:

杨林, 吴小候, 罗春丽, 等. 肾癌细胞来源的exosomes诱导Jurkat T细胞凋亡[J].第三军医大学学报,2013,35(5):426-430.

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更新日期/Last Update: 2013-03-05