[1]李沉纹,于彩平,李卓恒,等.金属离子对牛蒡子苷与牛血清白蛋白结合的影响[J].第三军医大学学报,2013,35(07):595-597.
 Li Chenwen,Yu Caiping,Li Zhuoheng,et al.Effect of metal ions on binding between arctiin and bovine serum albumin[J].J Third Mil Med Univ,2013,35(07):595-597.
点击复制

金属离子对牛蒡子苷与牛血清白蛋白结合的影响(/HTML )
分享到:

《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
35卷
期数:
2013年第07期
页码:
595-597
栏目:
论著
出版日期:
2013-04-15

文章信息/Info

Title:
Effect of metal ions on binding between arctiin and bovine serum albumin
作者:
李沉纹于彩平李卓恒管海燕罗明和卢来春
第三军医大学大坪医院野战外科研究所药剂科
Author(s):
Li Chenwen Yu Caiping Li Zhuoheng Guan Haiyan Luo Minghe Lu Laichun
Department of Pharmacy, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing, 400042, China
关键词:
牛血清白蛋白牛蒡子苷金属离子荧光增强
Keywords:
bovine serum albumin arctiin metal ion fluorescence enhancing
分类号:
R282.71; R341.21; R962
文献标志码:
A
摘要:
目的      研究金属离子对牛蒡子苷与牛血清白蛋白(bovine serum albumin, BSA)结合的影响。      方法       采用同步荧光光谱法,在拟生理pH条件下考察不同的金属离子(Cu2+、Mg2+、Zn2+)对牛蒡子苷与BSA结合作用的影响。      结果       在金属离子(Cu2+、Mg2+、Zn2+)存在下,随着体系中牛蒡子苷浓度增大,BSA荧光增强,两者间的结合力仍以静电作用力为主。金属离子存在时,牛蒡子苷与BSA的结合常数在25 ℃时,分别为7.899×104(无金属离子)、8.557×104(Cu2+)、6.724×104(Zn2+)、7.062×104(Mg2+);在37 ℃时,结合常数分别为5.962×104(无金属离子)、6.096×104(Cu2+)、5.915×104(Zn2+)、5.612×104(Mg2+)。      结论       金属离子的存在会影响牛蒡子苷与牛血清白蛋白的结合。Cu2+存在下,牛蒡子苷与BSA之间的结合常数增大;锌离子和镁离子存在下,两者的结合常数减小。
Abstract:
Objective       To determine the effect of metal ions on the binding between arctiin and bovine serum albumin (BSA).       Methods       Fluorescence spectroscopy was used to determine the effect of Cu2+, Mg2+, or Zn2+ on the interaction between arctiin and BSA under simulated physiological pH value.       Results       At the presence of metal ions (Cu2+, Mg2+, or Zn2+), BSA fluorescence was enhanced with the increase of the concentration of arctiin, with electro-static forces still playing a major role between them. The binding constant was 7.899×104 (without metal ion), 8.557×104(Cu2+), 6.724×104(Zn2+), and 7.062×104(Mg2+), respectively, at 25 ℃. And it was 5.962×104(without metal ion), 6.096×104(Cu2+), 5.915×104(Zn2+), and 5.612×104(Mg2+), respectively, at 37 ℃.       Conclusion       Metal ions affect the binding between arctiin and BSA. Among the metal ions, Cu2+ increases their binding constant, while, Mg2+ and Zn2+ decreases the binding constant.

参考文献/References:

李沉纹, 于彩平, 李卓恒, 等. 金属离子对牛蒡子苷与牛血清白蛋白结合的影响[J].第三军医大学学报,2013,35(7):595-597.

相似文献/References:

[1]魏运军,张学渊.豚鼠耳蜗微血管内皮细胞体外通透性的研究[J].第三军医大学学报,2002,24(09):0.[doi:10.16016/j.1000-5404.2002.09.025 ]
 WEI Yun jun,ZHANG Xue yuan.[J].J Third Mil Med Univ,2002,24(07):0.[doi:10.16016/j.1000-5404.2002.09.025 ]
[2]李素铠,欧阳斌,徐晓秋,等.PLGA纳米药物递送系统的建立及其对bFGF生物学活性的维持[J].第三军医大学学报,2016,38(11):1319.
 Li Sukai,Ouyang Bin,Xu Xiaoqiu,et al.Establishment of poly(DL-lactide-co-glycolide) nanoparticle drug delivery system and its maintenance for bioactivity of basic fibroblast growth factor[J].J Third Mil Med Univ,2016,38(07):1319.

更新日期/Last Update: 2013-04-07