[1]高诗豪,唐政恒,陈图南,等.蜕皮甾酮对大鼠脑组织自由基损伤的保护作用[J].第三军医大学学报,2012,34(23):2357-2359.
 Gao Shihao,Tang Zhengheng,Chen Tunan,et al.Ecdysterone protects brain injury against free radical in rats[J].J Third Mil Med Univ,2012,34(23):2357-2359.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
34卷
期数:
2012年第23期
页码:
2357-2359
栏目:
论著
出版日期:
2012-12-15

文章信息/Info

Title:
Ecdysterone protects brain injury against free radical in rats
作者:
高诗豪唐政恒陈图南李飞单佑安冯华
第三军医大学西南医院神经外科,全军神经外科研究所,全军神经创伤防治重点实验室
Author(s):
Gao Shihao Tang Zhengheng Chen Tunan Li Fei Shan You’an Feng Hua
Department of Neurosurgery, Institute of Neurosurgery, Key Laboratory of Neurotrauma Prevention and Treatment, Southwest Hospital, Third Military Medical University, Chongqing, 400038, China
关键词:
蜕皮甾酮脑损伤死亡率脑水肿自由基
Keywords:
ecdysterone traumatic brain injury death rate brain edema free radical
分类号:
R362; R651.15; R977
文献标志码:
A
摘要:
目的      观察蜕皮甾酮(ecdysterone, EDS)对大鼠脑损伤的治疗作用。      方法        SD雄性大鼠120只,按随机数字表法分6组,每组20只。采用Feeney法自由落体打击鼠脑造模。假手术组动物只开颅,不打击;治疗组:分别采用4、8、16 mg/kg的EDS腹腔注射模型动物;阴性治疗对照组:模型动物腹腔注射生理盐水;阳性治疗对照组:模型动物腹腔注射3 mg/kg依达拉奉。伤后7 d计算各组动物死亡率;检测各组动物脑组织含水量及丙二醛(MDA)、羟自由基能力及超(过)氧化物歧化酶(superoxide dismutase,SOD)等。HE染色观测各组大鼠脑组织海马病变情况。      结果        伤后7 d,16 mg/kg EDS治疗组的死亡率、脑组织含水量MDA与羟自由基含量较阴性治疗对照组明显降低(P<0.05),SOD活性显著升高(P<0.01),脑细胞损害明显减轻,大鼠海马CA1区正常神经元数量明显优于阴性治疗组(P<0.01)。      结论        蜕皮甾酮对脑损伤具有保护治疗作用。
Abstract:
Objective        To investigate the effect of ecdysterone (EDS) on traumatic brain injury (TBI) in rats.       Methods       A total of 120 male Sprague-Dawley rats were randomly and equally divided into 6 groups, sham-operation group, TBI group, edaravone treatment group and EDS treatment groups. Modified Feeney’s method was used to establish the rat model of experimental TBI with an impact of a weight of 30 g from 20 cm after craniotomy. Rats of EDS treatment groups were given EDS at 4, 8, and 16 mg/kg via peritoneal injection for 7 d. The other 3 groups were treated with normal saline or edaravone (3 mg/kg). The animal death rate was calculated, and the changes of the brain water content, malondialdehyde (MDA), and superoxide dismutase(SOD)in brain tissue were calculated and measured. Hematoxylin-eosin (HE) staining was used to observe the pathological changes of brain hippocampus tissue.       Results        EDS (16 mg/kg) resulted in significant decreases in the death rate, brain water content, and MDA content (P<0.05), and an obvious increase in SOD content (P<0.01) compared with TBI group. EDS (16 mg/kg) also resulted in lesser damage in the brain cells, with the pyramidal cells number significantly higher than TBI  group (P<0.01).       Conclusion        EDS exerts therapeutic effect on TBI through inhibiting free radical damage and brain edema.

参考文献/References:

高诗豪, 唐政恒, 陈图南, 等. 蜕皮甾酮对大鼠脑组织自由基损伤的保护作用[J]. 第三军医大学学报,2012,34(23):2357-2359.

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更新日期/Last Update: 2012-12-04