[1]邹哲华,陶陶,徐坚,等.大鼠大脑皮层神经元缺氧后细胞凋亡情况的动态观察[J].陆军军医大学学报(原第三军医大学学报),2012,34(24):2489-2492.
　Zou Zhehua,Tao Tao,Xu Jian,et al.Dynamic changes of apoptosis in rat cerebral cortex neurons after hypoxia[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(24):2489-2492.
点击复制

大鼠大脑皮层神经元缺氧后细胞凋亡情况的动态观察(/HTML )
分享到：

陆军军医大学学报（原第三军医大学学报）[ISSN:1000-5404/CN:51-1095/R]

卷:
34卷
期数:
2012年第24期
页码:
2489-2492
栏目:
论著
出版日期:
2012-12-30

文章信息/Info

Title:
Dynamic changes of apoptosis in rat cerebral cortex neurons after hypoxia
作者:
邹哲华; 陶陶; 徐坚; 刘智; 罗开俭
贵阳医学院：附属医院神经病学教研室，附属医院药剂科，肿瘤医院；河北省秦皇岛市第一医院老年病一科
Author(s):
Zou Zhehua;  Tao Tao;  Xu Jian;  Liu Zhi;  Luo Kaijian
Department of Neurology, Department of Pharmacy, Affiliated Hospital, Cancer Hospital, Guiyang Medical College, Guiyang, Guizhou Province, 550004; First Branch of Geriatrics Department, First Hospital of Qinhuangdao, Qinhuangdao, Hebei Province, 066000, China
关键词:
神经元; 缺氧; 凋亡; 电镜
Keywords:
neurons;  hypoxia;  apoptosis;  electron microscope
分类号:
R329.25；R338.1；R364.4
文献标志码:
A
摘要:
目的      观察大鼠大脑皮层神经元缺氧后细胞凋亡动态变化。      方法      制备大鼠大脑皮层神经元体外原代培养模型,免疫细胞化学鉴定大鼠大脑皮层神经元，透射电镜下观察不同时间点各实验组神经元超微结构的变化,TUNEL法观察不同时间点各实验组神经元凋亡情况。      结果      正常对照组神经元透射电镜下形态及染色质正常、内质网、线粒体等结构正常，缺氧组神经元水肿，细胞器破坏或消失；TUNEL法检测神经元凋亡：缺氧后各组神经元凋亡明显增加，与相应正常对照组相比有显著差异（P<0.01），缺氧48 h神经元凋亡达高峰（IOD为0.187±0.007），与缺氧12、24 h及72 h相比有显著差异（P<0.01）。      结论      细胞凋亡在缺血、缺氧性脑损伤中呈动态变化，是神经元死亡的重要形式。恰当时间窗内对神经元凋亡进行干预将是缺血、缺氧性脑病的有效治疗措施。
Abstract:
Objective      To observe the dynamic changes of apoptosis in rat cerebral cortex neurons after hypoxia.       Methods      Rat cerebral cortex neurons were primarily cultured from SD rats born within 24 h and then identified by immunocytochemical assay. Then the identified cells were cultured in the medium containing 100 μmol/L CoCl2 to simulate hypoxic condition. The cells cultivated in normal condition served as normal control (normoxia group). Ultrastructural changes of the neurons were observed by transmission electron microscopy (TEM). Neuronal apoptosis were observed by TUNEL assay.       Results      TEM displayed that the morphology of neurons was normal, so was the structure of chromatin, endoplasmic reticulum and mitochondria in normoxia group, while, cellular edema, organelle damage or disappearance were seen in the hypoxia group. TUNEL showed that obvious apoptosis were found in hypoxic cells, with significant difference with normoxia group (P<0.01). The apoptosis reached its peak in 48 h after hypoxia (0.187±0.007), significantly higher than those in 12, 24 and 72 h (P<0.01).       Conclusion      Apoptosis is a dynamic process in hypoxic-ischemic brain injury, and an important pattern of neuronal death. Intervention for neuronal apoptosis should be performed in an appropriate time window to effectively treat hypoxic-ischemic encephalopathy.

相似文献/References:

[1]周帅,蒋理,程崇杰,等.载脂蛋白E对星形胶质细胞缺氧性损伤的保护作用[J].陆军军医大学学报(原第三军医大学学报),2012,34(20):2067.
　Zhou Shuai,Jiang Li,Cheng Chongjie,et al.Apolipoprotein E protects hypoxic injuried astrocytes in vitro[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(24):2067.
[2]生宝亮,徐刚,陈德伟,等.大鼠实验性高原肺水肿中T-AOC、MDA、SOD、CAT和IL-6的表达[J].陆军军医大学学报(原第三军医大学学报),2012,34(23):2364.
　Sheng Baoliang,Xu Gang,Chen Dewei,et al.T-AOC，MDA，SOD，CAT and IL-6 levels in rat pulmonary edema induced by hypobaric hypoxia[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(24):2364.
[3]魏勇,顾健腾,鲁开智,等.瑞芬太尼对肝细胞缺氧复氧损伤保护作用的机制研究[J].陆军军医大学学报(原第三军医大学学报),2007,29(20):1976.
　WEI Yong,GU Jian-teng,LU Kai-zhi,et al.Remifentanyl protects hepatocytes against anoxia-reoxygenation injury[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(24):1976.
[4]杨兆瑞,吴晴,陈嘉薇,等.HIF-1α、JNK1、P-gp、MRP1和LRP蛋白在胃癌中的表达与临床病理及预后的关系[J].陆军军医大学学报(原第三军医大学学报),2007,29(18):1755.
　YANG Zhao-rui,WU Qing,CHEN Jia-wei,et al.Expressions, clinicopathological features and prognostic significance of HIF-1α, JNK1, P-gp, MRP1 and LRP proteins in human gastric carcinoma[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(24):1755.
[5]郑鸿燕,邰浩清,周春祥,等.蒺藜皂苷诱导SD新生大鼠海马神经干细胞分化的实验研究[J].陆军军医大学学报(原第三军医大学学报),2007,29(18):1764.
　ZHENG Hong-yan,TAI Hao-qing,ZHOU Chun-xiang,et al.Effects of total saponins of tribulus on the differentiation of rat neural hippocampus stem cells[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(24):1764.
[6]罗红,高文祥,高钰琪,等.茶多酚预防缺氧大鼠红细胞增多症的实验研究[J].陆军军医大学学报(原第三军医大学学报),2007,29(14):1381.
　LUO Hong,GAO Wen-xiang,GAO Yu-qi,et al.Protective effects of tea polyphenols on polycythemia induced by chronic hypoxia in rats[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(24):1381.
[7]刘茜,吴亚梅,徐玲,等.急性缺氧诱导C57BL/6J小鼠瘦素表达增加[J].陆军军医大学学报(原第三军医大学学报),2007,29(13):1311.
　LIU Xi,WU Ya-mei,XU Ling,et al.Influence of hypoxia on leptin expression in C57BL/6J mice[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(24):1311.
[8]秦伟,阴正勤,翁传煌,等.大鼠视皮层两类神经元的NMDA及GABAA受体介导的突触后电流电学特性研究[J].陆军军医大学学报(原第三军医大学学报),2008,30(08):714.
　QIN Wei,YIN Zheng-qin,WENG Chuan-huang,et al.Properties of the postsynaptic currents mediated by NMDA receptors or GABAA receptors recorded in the pyramidal or granular neurons of rat visual cortex[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(24):714.
[9]王国毅,陶国才,易斌,等.咪达唑仑对幼龄大鼠大脑神经元caspase-3活化的影响[J].陆军军医大学学报(原第三军医大学学报),2008,30(04):337.
　WANG Guo-yi,TAO Guo-cai,YI Bin,et al.Effect of Midazolam on caspase-3 activation of cerebral neurons in 7-day-old rats[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(24):337.
[10]张钢,高钰琪,刘福玉.缺氧对大鼠白细胞粘弹性的影响[J].陆军军医大学学报(原第三军医大学学报),2008,30(01):46.
　ZHANG Gang,GAO Yu-qi,LIU Fu-yu.Effects of hypoxia on viscoelastic properties of polymorphonuclear leukocyte of rats[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(24):46.
[11]李江涛,胡胜利,杜鹏,等.缺氧条件下大鼠神经元白细胞介素-1受体相关激酶-1的表达变化[J].陆军军医大学学报(原第三军医大学学报),2010,32(08):794.
　Li Jiangtao,Hu Shengli,Du Peng,et al.Hypoxia induces expression of interleukin-1 receptor-associated kinases-1 in rat neurons[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(24):794.
[12]黄河清,陈康宁,屠永华,等.培养神经元缺氧后PKC活性变化与细胞凋亡关系的研究[J].陆军军医大学学报(原第三军医大学学报),2002,24(12):0.[doi:10.16016/j.1000-5404.2002.12.011 ]
　HUANG He qing,CHEN Kang ning,TU Yong hua,et al.[J].J Amry Med Univ (J Third Mil Med Univ),2002,24(24):0.[doi:10.16016/j.1000-5404.2002.12.011 ]
[13]王中峰,黎海蒂,万子兵,等.脑缺氧诱导的大鼠大脑皮层神经元大电导NMDA受体通道开放[J].陆军军医大学学报(原第三军医大学学报),1999,21(07):0.[doi:10.16016/j.1000-5404.1999.07.009 ]
　Wang Zhongfeng,Li Haidi,Wan Zibing,et al.[J].J Amry Med Univ (J Third Mil Med Univ),1999,21(24):0.[doi:10.16016/j.1000-5404.1999.07.009 ]
[14]黄河清,陈康宁,邵淑琴,等.蛋白激酶C与培养神经元缺氧后caspase-3表达及细胞凋亡关系的研究[J].陆军军医大学学报(原第三军医大学学报),2001,23(01):0.[doi:10.16016/j.1000-5404.2001.01.016 ]
　HUANG He qing,CHEN Kang ning,SHAO Shu qin.[J].J Amry Med Univ (J Third Mil Med Univ),2001,23(24):0.[doi:10.16016/j.1000-5404.2001.01.016 ]

更新日期/Last Update: 2012-12-19