[1]邹哲华,陶陶,徐坚,等.大鼠大脑皮层神经元缺氧后细胞凋亡情况的动态观察[J].第三军医大学学报,2012,34(24):2489-2492.
　Zou Zhehua,Tao Tao,Xu Jian,et al.Dynamic changes of apoptosis in rat cerebral cortex neurons after hypoxia[J].J Third Mil Med Univ,2012,34(24):2489-2492.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
34卷
期数:
2012年第24期
页码:
2489-2492
栏目:
论著
出版日期:
2012-12-30

文章信息/Info

Title:
Dynamic changes of apoptosis in rat cerebral cortex neurons after hypoxia
作者:
邹哲华; 陶陶; 徐坚; 刘智; 罗开俭
贵阳医学院：附属医院神经病学教研室，附属医院药剂科，肿瘤医院；河北省秦皇岛市第一医院老年病一科
Author(s):
Zou Zhehua;  Tao Tao;  Xu Jian;  Liu Zhi;  Luo Kaijian
Department of Neurology, Department of Pharmacy, Affiliated Hospital, Cancer Hospital, Guiyang Medical College, Guiyang, Guizhou Province, 550004; First Branch of Geriatrics Department, First Hospital of Qinhuangdao, Qinhuangdao, Hebei Province, 066000, China
关键词:
神经元; 缺氧; 凋亡; 电镜
Keywords:
neurons;  hypoxia;  apoptosis;  electron microscope
分类号:
R329.25；R338.1；R364.4
文献标志码:
A
摘要:
目的      观察大鼠大脑皮层神经元缺氧后细胞凋亡动态变化。      方法      制备大鼠大脑皮层神经元体外原代培养模型,免疫细胞化学鉴定大鼠大脑皮层神经元，透射电镜下观察不同时间点各实验组神经元超微结构的变化,TUNEL法观察不同时间点各实验组神经元凋亡情况。      结果      正常对照组神经元透射电镜下形态及染色质正常、内质网、线粒体等结构正常，缺氧组神经元水肿，细胞器破坏或消失；TUNEL法检测神经元凋亡：缺氧后各组神经元凋亡明显增加，与相应正常对照组相比有显著差异（P<0.01），缺氧48 h神经元凋亡达高峰（IOD为0.187±0.007），与缺氧12、24 h及72 h相比有显著差异（P<0.01）。      结论      细胞凋亡在缺血、缺氧性脑损伤中呈动态变化，是神经元死亡的重要形式。恰当时间窗内对神经元凋亡进行干预将是缺血、缺氧性脑病的有效治疗措施。
Abstract:
Objective      To observe the dynamic changes of apoptosis in rat cerebral cortex neurons after hypoxia.       Methods      Rat cerebral cortex neurons were primarily cultured from SD rats born within 24 h and then identified by immunocytochemical assay. Then the identified cells were cultured in the medium containing 100 μmol/L CoCl2 to simulate hypoxic condition. The cells cultivated in normal condition served as normal control (normoxia group). Ultrastructural changes of the neurons were observed by transmission electron microscopy (TEM). Neuronal apoptosis were observed by TUNEL assay.       Results      TEM displayed that the morphology of neurons was normal, so was the structure of chromatin, endoplasmic reticulum and mitochondria in normoxia group, while, cellular edema, organelle damage or disappearance were seen in the hypoxia group. TUNEL showed that obvious apoptosis were found in hypoxic cells, with significant difference with normoxia group (P<0.01). The apoptosis reached its peak in 48 h after hypoxia (0.187±0.007), significantly higher than those in 12, 24 and 72 h (P<0.01).       Conclusion      Apoptosis is a dynamic process in hypoxic-ischemic brain injury, and an important pattern of neuronal death. Intervention for neuronal apoptosis should be performed in an appropriate time window to effectively treat hypoxic-ischemic encephalopathy.

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更新日期/Last Update: 2012-12-19