[1]蒋平,关伟,戴楠,等.DNA损伤修复基因以及TS、β-tubulinⅢ在骨肉瘤中的表达及其与临床病理的关系[J].陆军军医大学学报(原第三军医大学学报),2013,35(01):61-65.
 Jiang Ping,Guan Wei,Dai Nan,et al.Expression of DNA damage repair genes, thymidylate synthase and β-tubulin Ⅲ in human osteosarcoma tissues and its relationship with clinical pathology of osteosarcoma[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(01):61-65.
点击复制

DNA损伤修复基因以及TS、β-tubulinⅢ在骨肉瘤中的表达及其与临床病理的关系(/HTML )
分享到:

陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
35卷
期数:
2013年第01期
页码:
61-65
栏目:
论著
出版日期:
2013-01-15

文章信息/Info

Title:
Expression of DNA damage repair genes, thymidylate synthase and β-tubulin Ⅲ in human osteosarcoma tissues and its relationship with clinical pathology of osteosarcoma
作者:
蒋平关伟戴楠仲召阳毛承毅顾咸庆王东
第三军医大学大坪医院野战外科研究所肿瘤中心
Author(s):
Jiang Ping Guan Wei Dai Nan Zhong Zhaoyang Mao Chengyi Gu Xianqing Wang Dong
Cancer Center, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing, 400042, China
关键词:
骨肉瘤DNA损伤修复基因组织芯片免疫组织化学
Keywords:
osteosarcoma DNA damage repair gene tissue microarray immunohistochemistry
分类号:
R394.2; R730.23; R738.1
文献标志码:
A
摘要:
目的      探讨人骨肉瘤组织中DNA损伤修复基因以及TS、β-tubulinⅢ多个化疗反应相关标志物的表达谱特点,及其与临床病理的关系。       方法        选取50例诊断明确的骨肉瘤患者组织蜡块,运用组织芯片和免疫组化技术检测OGG1、BRCA1、RRM1、XRCC1、APE1、ERCC1以及TS、β-tubulinⅢ 8种基因在骨肉瘤组织中的表达情况。       结果        ①OGG1、BRCA1、RRM1、XRCC1、APE1、ERCC1在骨肉瘤组织中呈阳性表达,阳性率分别为96%、94%、94%、84%、84%、74%,强阳性率为64%、76%、54%、72%、48%、2%,而TS仅有6例有弱阳性表达,β-tubulinⅢ无阳性表达。②阳性表达的基因中,仅APE1表达与骨肉瘤的组织病理类型具有相关性(P<0.05)。③等级相关分析,APE1和RRM1、APE1和OGG1、OGG1和BRCA1表达呈正相关(r=0.342、0.318、0.319,P<0.05),BRCA1和ERCC1表达呈负相关(r=-0.324,P<0.05);APE1和XRCC1、RRM1和XRCC1的表达也呈正相关(r=0.406、0.677,P<0.01)。      结论      骨肉瘤组织中存在多种DNA损伤修复相关基因的表达,其中部分基因的表达强弱之间具有相关性,APE1的表达强弱与肺癌病理类型有关。
Abstract:
Objective        To explore the expression profiles of multiple chemotherapy-related markers of DNA damage repair genes and thymidylate synthase (TS) β-tubulin Ⅲ in human osteosarcoma tissues and their relationship with clinical pathology of osteosarcoma.       Methods       Fifty cases of osteosarcoma tissue paraffin blocks were obtained from the patients with diagnosed osteosarcoma. Tissue microarray and immunohistochemical method were applied to detect gene expression levels of OGG1, BRCA1, RRM1, XRCC1, APE1, ERCC1, TS and β-tubulin Ⅲ in the osteosarcoma tissues.       Results        ①OGG1, BRCA1, RRM1, XRCC1, APE1 and ERCC1 were positively expressed in osteosarcoma tissues with the positive rates of 96%, 94%, 94%, 84%, 84% and 74%, respectively, and their strongly positive rates were 64%, 76%, 54%, 72%, 48% and 2%, respectively. There were only 6 cases showed weakly positive expression of TS, and β-tubulin Ⅲ was negative in osteosarcoma tissues. ②In the positively expressed genes, only APE1 expression was correlated with osteosarcoma pathological types (P<0.05). ③The results of rank correlation analysis showed that APE1 expression was positively correlated with the expression of RRM1, OGG1 and XRCC1 (P<0.05, P<0.01), OGG1 expression was positively correlated with BRCA1 expression (P<0.05), RRM1 expression was positively correlated with and XRCC1 expression (P<0.01), but the expression of BRCA1 was negatively correlated with ERCC1 expression (P<0.05).       Conclusion        Several types of DNA damage repair genes are expressed in osteosarcoma tissues, and some of their expression levels have Spearman rank correlations. The characteristics of the chemotherapy-related gene expression in osteosarcoma may have significance for individual chemotherapy in patients with osteosarcoma.

相似文献/References:

[1]温磊,孟刚,李懿,等.人骨肉瘤抗失巢凋亡细胞的生物学特性研究[J].陆军军医大学学报(原第三军医大学学报),2008,30(10):942.
 WEN Lei,MENG Gang,LI Yi,et al.Biological characteristics of anoikis-resistant cells from human osteosarcoma cell line hFOB1.19[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(01):942.
[2]李懿,孟刚,郭乔楠.P53和P63蛋白在人骨肉瘤组织及恶性转化细胞株中的表达变化及意义[J].陆军军医大学学报(原第三军医大学学报),2006,28(08):763.
[3]仲召阳,王东,李增鹏,等.骨肉瘤中APE1的表达及其与血管生成的关系[J].陆军军医大学学报(原第三军医大学学报),2005,27(10):1045.
[4]卫佳,李星星,陈英华,等.β-catenin降低外源性hS100A6对骨肉瘤细胞的抑制作用[J].陆军军医大学学报(原第三军医大学学报),2010,32(16):1703.
 Wei Jia,Li Xingxing,Chen Yinghua,et al.β-catenin decreases hS100A6-induced inhibition in osteosarcoma cell lines MG63 and U2OS[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(01):1703.
[5]王东,仲召阳,李增鹏,等.骨肉瘤中ephrinB2/EphB4的表达及其与血管生成的关系[J].陆军军医大学学报(原第三军医大学学报),2004,26(22):0.[doi:10.16016/j.1000-5404.2004.22.024 ]
 WANG Dong,ZHONG Zhao-yang,LI Zeng-peng,et al.[J].J Amry Med Univ (J Third Mil Med Univ),2004,26(01):0.[doi:10.16016/j.1000-5404.2004.22.024 ]
[6]何畔,梁珊.RNAi抑制XIAP基因诱导MG63细胞凋亡、化疗增敏及其分子机制[J].陆军军医大学学报(原第三军医大学学报),2010,32(12):1312.
 He Pan,Liang Shan.RNAi silencing of XIAP gene induces apoptosis and susceptibility to chemotherapy of osteosarcoma cell line MG63[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(01):1312.
[7]曾静,温磊,黄玉胜,等.神经营养因子酪氨酸激酶受体B对人永生化成骨细胞hFOB1.19恶性转化细胞体外侵袭力的影响[J].陆军军医大学学报(原第三军医大学学报),2010,32(09):938.
 Zeng Jing,Wen Lei,Huang Yusheng,et al.Effect of neurotrophic tyrosine kinase receptor B on in vitro invasiveness of malignant transformed hFOB1.19 cells[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(01):938.
[8]洪思琦,毕杨,郭振华,等.携带siANGPTL4重组腺病毒的构建及其对MG63增殖的抑制作用[J].陆军军医大学学报(原第三军医大学学报),2011,33(01):28.
 Hong Siqi,Bi Yang,Guo Zhenhua,et al.Construction of recombinant adenovirus with siANGPTL4 gene and its inhibitive effect on MG63 cell proliferation[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(01):28.
[9]周斌,李宏维,史继德,等.PNKP沉默对骨肉瘤放疗的增敏作用[J].陆军军医大学学报(原第三军医大学学报),2016,38(04):390.
 Zhou Bin,Li Hongwei,Shi Jide,et al.PNKP silencing increases radiosensitivity of osteosarcoma cells in vitro[J].J Amry Med Univ (J Third Mil Med Univ),2016,38(01):390.
[10]孟刚,李懿,郭乔楠.高密度Oligo基因芯片筛选人永生化成骨细胞恶性转化中印迹基因的研究[J].陆军军医大学学报(原第三军医大学学报),2007,29(08):666.
 MENG Gang,LI Yi,GUO Qiao-nan.Screening imprinted genes during malignant transformation of immortalized human osteoblastic cells by large-scale oligomicroarray technique[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(01):666.

更新日期/Last Update: 2012-12-31