[1]李铭,陈幸生,郭志辉,等.口服普萘洛尔对婴幼儿血管瘤MMP-2、MMP-9、TIMP-1、TIMP-2表达水平的影响[J].陆军军医大学学报(原第三军医大学学报),2012,34(23):2403-2407.
 Li Ming,Chen Xingsheng,Guo Zhihui,et al.Effect of propranolol on expression of MMP-2, MMP-9, TIMP-1 and TIMP-2 in infantile hemangioma[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(23):2403-2407.
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陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
34卷
期数:
2012年第23期
页码:
2403-2407
栏目:
论著
出版日期:
2012-12-15

文章信息/Info

Title:
Effect of propranolol on expression of MMP-2, MMP-9, TIMP-1 and TIMP-2 in infantile hemangioma
作者:
李铭陈幸生郭志辉谢义德詹明坤周亚宽江成鸿陈小松
福建医科大学附属协和医院:整形外科,血管甲状腺外科
Author(s):
Li Ming Chen Xingsheng Guo Zhihui Xie Yide Zhan Mingkun Zhou Yakuan Jiang Chenghong Chen Xiaosong
Department of Plastic Surgery, Department of Vascular & Thyroid Surgery, Affiliated Fujian Union Hospital, Fujian Medical University,  Fuzhou, Fujian Province, 350001, China
关键词:
血管瘤普萘洛尔MMP-2MMP-9TIMP-1TIMP-2
Keywords:
hemangioma propranolol MMP-2 MMP-9 TIMP-1 TIMP-2
分类号:
R732.2; R966; R972
文献标志码:
A
摘要:
目的      研究口服普萘洛尔前后婴幼儿血管瘤病灶内MMP-2、MMP-9、TIMP-1、TIMP-2表达水平的影响。      方法        选择2010年5月至2011年12月来我院就诊、年龄≤3个月、血管瘤病灶位于肢体或隐蔽部位、家属要求手术治疗却具备单独口服普萘洛尔治疗条件、排除口服普萘洛尔禁忌证、先前未接受过其他任何相关治疗的病例作为研究对象,共39例。经过与家属沟通并征得其书面同意,先按口服普萘洛尔诊疗常规,口服用药前在局麻下夹取血管瘤体组织活检,后口服8周普萘洛尔[剂量:2 mg/(kg·d),分2次,每12小时用药],继而手术切除瘤体。采用免疫组化、实时荧光定量RT-PCR 的方法,检测口服用药前后病灶组织内MMP-2、MMP-9、TIMP-1、TIMP-2表达水平的变化,计算口服用药前后病灶微血管密度(MVD)。      结果        免疫组化与实时荧光定量RT-PCR结果均提示,口服普萘洛尔后血管瘤病灶内MVD值明显减低且具有统计学意义(t=18.458,P<0.01), MMP-2、MMP-9表达水平较用药前明显减少(P<0.01),TIMP-1、TIMP-2表达水平明显增高(P<0.01)。且口服用药后上述4个因子免疫组化平均积分光密度、mRNA相对表达量变化与MVD值变化密切相关。      结论        普萘洛尔可下调血管瘤病灶内MMP-2、MMP-9的表达,上调TIMP-1、TIMP-2的表达,从而抑制新生血管的形成。
Abstract:
Objective        To investigate the effect of propranolol on the expression of matrix metalloproteinase 2 (MMP-2), matrix metalloproteinase 9 (MMP-9), tissue inhibitor of metalloproteinase 1 (TIMP-1) and tissue inhibitor of metalloproteinase 2 (TIMP-2) in infantile hemangioma.       Methods        Thirty-nine patients (age≤3 months) with proliferative phase of hemangioma in our hospital from May, 2010 to December, 2010 were enrolled. The patients were treated with propranolol for 8 weeks after hemangioma biopsy, and then underwent surgery for hemangioma resection. The mRNA and protein expression levels of MMP-2, MMP-9, TIMP-1 and TIMP-2 in hemangioma tissues were examined by quantitative real-time RT-PCR and immunohistochemical method, respectively, and the microvessel density (MVD) was calculated before and after propranolol treatment.       Results        The MVD in the hemangioma tissues decreased significantly after propranolol treatment (P<0.01). The protein and mRNA expressions of MMP-2 and MMP-9 decreased significantly (P<0.01), but the protein and mRNA expressions of TIMP-1 and TIMP-2 increased significantly after propranolol treatment (P<0.01). The changes of MVD were closely correlated with the protein and mRNA expression levels of MMP-2, MMP-9, TIMP-1 and TIMP-2.       Conclusion        Propranolol can reduce proliferative hemangioma through down-regulating MMP-2 and MMP-9 expression and up-regulating TIMP-1 and TIMP-2 expression.

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更新日期/Last Update: 2012-12-04