[1]生宝亮,徐刚,陈德伟,等.大鼠实验性高原肺水肿中T-AOC、MDA、SOD、CAT和IL-6的表达[J].第三军医大学学报,2012,34(23):2364-2367.
 Sheng Baoliang,Xu Gang,Chen Dewei,et al.T-AOC,MDA,SOD,CAT and IL-6 levels in rat pulmonary edema induced by hypobaric hypoxia[J].J Third Mil Med Univ,2012,34(23):2364-2367.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
34卷
期数:
2012年第23期
页码:
2364-2367
栏目:
论著
出版日期:
2012-12-15

文章信息/Info

Title:
T-AOC,MDA,SOD,CAT and IL-6 levels in rat pulmonary edema induced by hypobaric hypoxia
作者:
生宝亮徐刚陈德伟陈建高文祥李晓栩杨娟刘福玉高钰琪
第三军医大学高原军事医学系病理生理与高原生理学教研室,教育部高原医学重点实验室,全军高原医学重点实验室
Author(s):
Sheng Baoliang Xu Gang Chen Dewei Chen Jian Gao Wenxiang Li Xiaoxu Yang Juan Liu Fuyu Gao Yuqi
Department of Pathophysiology and High Altitude Physiology, Key Laboratory of High Altitude Medicine of Ministry of Education, College of High Altitude Military Medicine, Third Military Medical University, Chongqing, 400038, China
关键词:
缺氧高原肺水肿氧化应激
Keywords:
hypoxiahigh-altitude pulmonary edemaoxidative stress
分类号:
R362;R364.4;R563.9
文献标志码:
A
摘要:
目的      观察氧化应激反应在急性缺氧致大鼠高原肺水肿发生中的变化,并探讨其病理生理学意义。      方法        成年雄性SD大鼠72只,体质量(200 ±20)g,按随机数字表法分为平原对照(C)组、模拟急性高原缺氧(H)6、12、24、48和72 h组(n=12)。H组动物置于减压舱内模拟海拔6 000 m高原暴露相应时间;分别在平原和模拟高原环境取材,检测肺组织湿干质量比值、总抗氧化能力(total-antioxidant capacity,T-AOC)、丙二醛(malondialdehyde,MDA)、超氧化物歧化酶(superoxide dismutase,SOD)、过氧化氢酶(catalase,CAT)、血浆及肺组织IL-6。      结果       与平原对照组比较:缺氧各组大鼠肺组织湿干质量比值均显著增加(P<0.05,P<0.01);MDA随缺氧时间延长逐渐增加,缺氧72 h达到最高(P<0.01);SOD在缺氧12、24 h显著降低(P<0.05),在缺氧48、72 h进一步降低(P<0.01);T-AOC、CAT随缺氧时间延长在24、48、72 h显著降低(P<0.05);血浆IL-6在缺氧24、48、72 h组显著增加(P<0.01),肺组织IL-6在缺氧48(P<0.01)、72 h(P<0.05)组显著增加。      结论       急性缺氧诱导大鼠高原肺水肿的发生和氧化应激有关,机体抗氧化能力降低、自由基增加是HAPE发生的重要机制。
Abstract:
Objective        To observe the changes of oxidative stress in the occurrence of pulmonary edema in rats induced by hypoxia and to investigate the pathophysiological significance.        Methods        A total of 72 adult male SD rats weighting 200±20 g were randomly divided into control group (C), and simulated altitude acute hypoxia groups (H). The hypoxia group rats were exposed to a simulated altitude of 6 000 m  for 6, 12, 24, 48 and 72 h respectively. The lung wet/dry (W/D) ratio, total-antioxidant capacity (T-AOC), malondialdehyde (MDA) content, activity of superoxide dismutase (SOD) and catalase (CAT) were measured. Lung and plasma IL-6 were measured by ELISA.       Results        The W/D ratio was increased in hypoxia groups than in control group (H6, H12 and H24 vs C, P<0.01, H48 and H72 vs C, P<0.05). MDA content was gradually increased with the prolongation of hypoxia time, and reached the highest in 72 h after hypoxic exposure (P<0.01). SOD content was significantly deceased in 12 and 24 h after hypoxic exposure(P<0.05) and further reduced in 48 and 72 h(P<0.01). Contents of T-AOC and CAT were significantly reduced with the prolongation of hypoxia time in 24, 48 and 72 h(P<0.05). Plasma IL-6 was significantly increased in hypoxic 24, 48 and 72 h groups (P<0.01), while that in lung tissue was significantly increased in hypoxic 48 and 72 h groups compared with the control group(H48 vs C, P<0.01; H72 vs C, P<0.05).       Conclusion       Acute hypoxia-induced high altitude pulmonary edema is related to oxidative stress. Decrease of antioxidant capacity and increase of free radicals contribute to the occurrence of high altitude pulmonary edema.

参考文献/References:

生宝亮, 徐刚, 陈德伟, 等. 大鼠实验性高原肺水肿中T-AOC、MDA、SOD、CAT和IL-6的表达[J]. 第三军医大学学报,2012,34(23):2364-2367.

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更新日期/Last Update: 2012-12-04