[1]蒙颖,徐芳,王志禄,等.罗格列酮对泡沫细胞中胆固醇贮存与运输相关蛋白ACAT-1、ABCA-1表达的影响[J].第三军医大学学报,2012,34(22):2288-2291.
 Meng Ying,Xu Fang,Wang Zhilu,et al.Effect of rosiglitazone on expression of acyl-coenzyme A cholesterol acyltransferase 1 and ATP-binding cassette transporter A1 in foam cells[J].J Third Mil Med Univ,2012,34(22):2288-2291.
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罗格列酮对泡沫细胞中胆固醇贮存与运输相关蛋白ACAT-1、ABCA-1表达的影响(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
34卷
期数:
2012年第22期
页码:
2288-2291
栏目:
论著
出版日期:
2012-11-30

文章信息/Info

Title:
Effect of rosiglitazone on expression of acyl-coenzyme A cholesterol acyltransferase 1 and ATP-binding cassette transporter A1 in foam cells
作者:
蒙颖徐芳王志禄李万玲贾军正郭文芬谢宛霞胡海英胡旭堂
兰州大学:第一临床医学院心内科,第一医院心内科
Author(s):
Meng Ying Xu Fang Wang Zhilu Li Wanling Jia Junzheng Guo Wenfen Xie Wanxia Hu Haiying Hu Xutang
Department of Cardiology, First Clinical Medical College of Lanzhou University,Department of Cardiology, First Hospital of Lanzhou University, Lanzhou, Gansu Province, 730000, China
关键词:
罗格列酮泡沫细胞胆固醇动脉粥样硬化
Keywords:
rosiglitazone foam cell cholesterol atherosclerosis
分类号:
R543.5;R966;R977.15
文献标志码:
A
摘要:
目的      观察罗格列酮对RAW264.7巨噬源性泡沫细胞形成中胆固醇含量及酰基辅酶A-胆固醇酰基转移酶-1(acyl coenzyme A cholesterol acyl transfer enzyme 1,ACAT-1)、三磷酸腺苷结合盒转运蛋白A-1 (ATP combination box of transporter 1,ABCA-1) 表达的影响,探讨罗格列酮对泡沫细胞形成的影响及可能的作用机制。      方法      在DMEM高糖培养基中培养RAW264.7巨噬细胞,按完全随机分组方法分为:①空白对照组(常规培养基培养巨噬细胞);②氧化低密度脂蛋白(oxidized low density lipoprotein,oxLDL)组(用终浓度为30 mg/L的oxLDL孵育48 h);③oxLDL+罗格列酮组(分别用终浓度5、10、20 μmol/L的罗格列酮+30 mg/L oxLDL共同孵育48 h)(n=10)。采用油红O染色观察泡沫细胞,胆固醇检测试剂盒测定各组细胞内总胆固醇(total cholesterol,TC)和游离胆固醇(free cholesterol,FC)的含量,Western blot法检测各组细胞ACAT-1和ABCA1的表达水平。      结果      oxLDL组中大量泡沫细胞的胞质被油红O染色,oxLDL+罗格列酮组泡沫细胞胞质染色明显浅于oxLDL组的细胞;与oxLDL组相比,oxLDL+罗格列酮组TC及FC显著降低(P<0.05),且呈浓度依赖性;Western blot检测结果表明,不同浓度(5、10、20 μmol/L)oxLDL+罗格列酮组ACAT-1蛋白表达分别为(0.94±0.11)、(0.86±0.13)、(0.58±0.12),与oxLDL组(1.19±0.12)相比有显著差异(P<0.05),不同浓度oxLDL+罗格列酮组ABCA1蛋白表达分别为(0.72±0.08)、(0.91±0.15)、(1.15±0.11),与oxLDL组(0.63±0.05)相比有显著差异(P<0.05),且呈浓度依赖性。      结论      罗格列酮可能通过抑制ACAT-1的表达及促进ABCA-1的表达减少泡沫细胞形成,从而发挥其抗动脉粥样硬化的作用。
Abstract:
Objective      To investigate the effect of rosiglitazone on cholesterol content and the expression of acyl-coenzyme A cholesterol acyltransferase 1 (ACAT1) and ATP-binding cassette transporter A1 (ABCA1) in RAW264.7 macrophage-derived foam cells, and to explore the mechanism of rosiglitazone in foam cell formation.       Methods      Mouse macrophage RAW264.7 cells were cultured in high-glucose DMEM and were randomly divided into a control group, an oxidized low-density lipoprotein (oxLDL) group, in which cells were incubated with 30 mg/L oxLDL for 48 h, and an oxLDL+rosiglitazone group, in which cells were incubated with 30 mg/L oxLDL plus 5, 10 and 20 μmol/L rosiglitazone for 48 h, respectively (n=10). The formation of foam cells were identified by oil red O staining. The levels of intracellular total cholesterol (TC) and free cholesterol (FC) in each group were determined by a cholesterol detection kit, and the expression of ACAT-1 and ABCA-1 protein was determined by Western blotting.       Results      Mouse macrophage RAW264.7 cells in the oxLDL group were stained by oil red O, but the color of the macrophages in the oxLDL+rosiglitazone group was lighter than that in the oxLDL group. Compared with the oxLDL group, the contents of intracellular TC and FC in the oxLDL+rosiglitazone group significantly decreased (P<0.05) in a dose-dependent manner. Western blot results showed the expression levels of ACAT-1 in the oxLDL+rosiglitazone group with different concentrations of rosiglitazone (5, 10 and 20 μmol/L) were (0.94±0.11), (0.86±0.13) and (0.58±0.12), respectively, which were significantly different from that in the oxLDL group (1.19±0.12) (P<0.05). The expression levels of ABCA-1 in the oxLDL+rosiglitazone group with different concentrations of rosiglitazone (5, 10 and 20 μmol/L) were (0.72±0.08), (0.91±0.15) and (1.15±0.11), respectively, which were also significantly different from that in the oxLDL group (0.63±0.05) (P<0.05).       Conclusion      Rosiglitazone may inhibit foam cell formation by decreasing the expression of ACAT-1 and increasing the expression of ABCA-1, which plays an important role in inhibiting atherosclerosis.

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更新日期/Last Update: 2012-11-20