[1]吴登艳,宋娇,董海良,等.mTOR信号通路介导黄芩苷抑制人结肠癌细胞的增殖[J].第三军医大学学报,2012,34(23):2399-2402.
 Wu Dengyan,Song Jiao,Dong Hailiang,et al.mTOR signaling pathway mediates baicalin-inhibited proliferation in human colon cancer cell line HCT116[J].J Third Mil Med Univ,2012,34(23):2399-2402.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
34卷
期数:
2012年第23期
页码:
2399-2402
栏目:
论著
出版日期:
2012-12-15

文章信息/Info

Title:
mTOR signaling pathway mediates baicalin-inhibited proliferation in human colon cancer cell line HCT116
作者:
吴登艳宋娇董海良邢伟徐祥黄宏郝进
重庆医科大学附属第二医院皮肤科;第三军医大学大坪医院野战外科研究所第一研究室,创伤、烧伤与复合伤国家重点实验室
Author(s):
Wu Dengyan Song Jiao Dong Hailiang Xing Wei Xu Xiang Huang Hong Hao Jin
Department of Dermatology, Second Affiliated Hospital, Chongqing Medical University, Chongqing, 400010;State Key Laboratory of Trauma, Burns and Combined Injury, Department 1, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing, 400042, China
关键词:
黄芩苷人结直肠癌HCT116细胞增殖mTOR信号通路
Keywords:
baicalin human colon cancer cell line HCT116 proliferation mTOR signaling pathway
分类号:
R285.5; R730.23; R735.3
文献标志码:
A
摘要:
目的      探讨黄芩苷抑制人结肠癌细胞(HCT116)增殖的分子机制。      方法        采用MTT比色法检测不同浓度的黄芩苷(0.01~100 μg/ml)对体外培养的HCT116细胞增殖的抑制作用;采用Western blot分析检测被黄芩苷干预的HCT 116细胞的增殖相关蛋白的表达。      结果        与对照组比较,浓度为1~100 μg/ml的黄芩苷处理组明显抑制HCT 116细胞的增殖(P<0.05)。用100 μg/ml黄芩苷处理后,增殖相关的蛋白mTOR、p70S6K、S6、eIF4E表达明显下调(P<0.05),4E-BP1表达明显上调。p-mTOR(Ser2448)、p-S6(Ser235/236)、p-4E-BP1(Thr37/46)的磷酸化水平下降(P<0.05)。      结论        黄芩苷能通过抑制mTOR信号通路来抑制人结直肠癌HCT116细胞的增殖。
Abstract:
Objective        To determine the inhibitory effect of baicalin on the proliferation of human colon cancer cell line HCT116 and its underlying mechanism.       Methods        Human colon cancer cell line HCT116 were primarily cultured in vitro and treated with baicalin at different concentrations of 0.01 to 100 μg/ml. MTT assay was performed to assess the cell proliferation. Western blotting was used to analyze the expression of cell proliferation related proteins in HCT116 cells after baicalin treatment.       Results        Baicalin from 1 to 100 μg/ml resulted in significantly inhibitory effect on HCT116 cells proliferation compared with the control group (P<0.05). The protein expression of mTOR, p70S6K,S6 and eIF4E were significantly down-regulated by baicalin (100 μg/ml) (P<0.05), as well as the expression of 4E-BP1 was significantly up-regulated. The phosphorylation of mTOR, S6 and 4E-BP1 were suppressed by baicalin (100 μg/ml) in HCT116 cells (P<0.05).       Conclusion        Baicalin inhibited the proliferation in HCT116 cells through mTOR signaling pathway.

参考文献/References:

吴登艳, 宋娇, 董海良, 等. mTOR信号通路介导黄芩苷抑制人结肠癌细胞的增殖[J]. 第三军医大学学报,2012,34(23):2399-2402.

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更新日期/Last Update: 2012-12-04