[1]夏蕾,熊艳丽,罗维,等.染料木黄酮对辐射损伤小鼠肺纤维化的防护作用[J].陆军军医大学学报(原第三军医大学学报),2012,34(21):2149-2153.
 Xia Lei,Xiong Yanli,Luo Wei,et al.Genistein protects mice against radiation-induced pulmonary fibrosis[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(21):2149-2153.
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染料木黄酮对辐射损伤小鼠肺纤维化的防护作用(/HTML )
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陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
34卷
期数:
2012年第21期
页码:
2149-2153
栏目:
论著
出版日期:
2012-11-15

文章信息/Info

Title:
Genistein protects mice against radiation-induced pulmonary fibrosis
作者:
夏蕾 熊艳丽 罗维 李娟 肖何 王阁 王东 杨镇洲
第三军医大学大坪医院野战外科研究所肿瘤中心
Author(s):
Xia Lei Xiong Yanli Luo Wei Li Juan Xiao He Wang Ge Wang Dong Yang Zhenzhou
Center of Cancer, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing, 400042, China
关键词:
放射性肺纤维化染料木黄酮羟脯氨酸TGF-β1
Keywords:
radiation-induced pulmonary fibrosis genistein transforming growth factor β1 hydroxyprolin
分类号:
R563; R818.74; R979.6
文献标志码:
A
摘要:
目的      探讨染料木黄酮 (genistein, GEN) 对小鼠放射性肺纤维化(radiation-induced pulmonary fibrosis, RIPF)的保护作用。      方法        60只8周龄C57BL/6J雌性小鼠,随机分为对照组(C组)、单纯照射组(R组)、染料木黄酮+照射组(Gen+R组)和氨磷汀 (Amifostine)+照射组(Ami+R组)(n=10)。除对照组外,其余各组小鼠接受8MV X线全胸照射(12.0±0.2) Gy。Gen+R组于照射开始前24、16、8 h皮下注射GEN 200 mg/kg,Ami+R组于照射开始前30 min皮下注射氨磷汀100 mg/kg,照射后每天1次连续给药4周。照射后4、8、12周,HE染色观察肺组织病理改变,ELISA方法测定血清中的TGF-β1的含量,比色法测定肺组织羟脯氨酸的含量。      结果        病理检测显示照射后时间越长,放射性肺损伤小鼠肺纤维化越重,染料木黄酮能减轻肺组织炎症及纤维化;照射后小鼠血清TGF-β1和肺组织羟脯氨酸的含量也呈递增趋势,染料木黄酮能降低TGF-β1和羟脯氨酸的含量(P<0.05),尤其是照射前24 h给予染料木黄酮能达到与氨磷汀类似的疗效。      结论       染料木黄酮可减轻肺组织的纤维化,具有明显的辐射防护作用。
Abstract:
Objective       To evaluate the effect of genistein as a protecting agent on the radiation-induced pulmonary fibrosis in mice.       Methods        The thoraces of female 8-week-old C57BL/6J mice were exposed to either sham irradiation or single fraction of 8MV X ray radiation of (12.0±0.2) Gy. A total of 60 mice were divided as Sham (C group, n=10), radiation (R group, n=10), genistein+radiation (Gen+R group, n=30), amifostine+radiation (Ami+R group, n=10). The mice of Gen+R group were administered with genistein (200 mg/kg) in 8, 16 and 24 h respectively before irradiation (n=10) and then administered once per day until 4 weeks. While the mice of Ami+R group was injected subcutaneously by 100 mg/kg amifostine in 30 min before irradiation and in every day until 4 weeks. Mice were sacrificed at 4, 8, and 12 weeks post-irradiation. The lung tissues were removed and processed for definitive analysis, including hydroxypmline content and HE staining. ELISA was used to measure the serum level of transforming growth factor β1 (TGF-β1).       Results       Pathological study showed that pulmonary fibrosis became more severe along with the time after irradiation, and genistein attenuated lung inflammation and fibrosis. Radiation increased the expression of TGF-β1 and hydroxyprolin content in lung tissue in a time-dependent manner, and genistein reduced TGF-β1 expression and hydroxyprolin content (P<0.05).       Conclusion       Genistein attenuates the progress of radiation-induced lung fibrosis, and exerts significant effect on prevention against radiation.

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更新日期/Last Update: 2012-11-06