[1]夏蕾,熊艳丽,罗维,等.染料木黄酮对辐射损伤小鼠肺纤维化的防护作用[J].陆军军医大学学报(原第三军医大学学报),2012,34(21):2149-2153.
　Xia Lei,Xiong Yanli,Luo Wei,et al.Genistein protects mice against radiation-induced pulmonary fibrosis[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(21):2149-2153.
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染料木黄酮对辐射损伤小鼠肺纤维化的防护作用(/HTML )
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陆军军医大学学报（原第三军医大学学报）[ISSN:1000-5404/CN:51-1095/R]

卷:
34卷
期数:
2012年第21期
页码:
2149-2153
栏目:
论著
出版日期:
2012-11-15

文章信息/Info

Title:
Genistein protects mice against radiation-induced pulmonary fibrosis
作者:
夏蕾;  熊艳丽;  罗维;  李娟;  肖何;  王阁;  王东;  杨镇洲
第三军医大学大坪医院野战外科研究所肿瘤中心
Author(s):
Xia Lei;  Xiong Yanli;  Luo Wei;  Li Juan;  Xiao He;  Wang Ge;  Wang Dong;  Yang Zhenzhou
Center of Cancer, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing, 400042, China
关键词:
放射性肺纤维化; 染料木黄酮; 羟脯氨酸; TGF-β1
Keywords:
radiation-induced pulmonary fibrosis;  genistein;  transforming growth factor β1;  hydroxyprolin
分类号:
R563; R818.74; R979.6
文献标志码:
A
摘要:
目的      探讨染料木黄酮 (genistein, GEN) 对小鼠放射性肺纤维化（radiation-induced pulmonary fibrosis, RIPF）的保护作用。      方法        60只8周龄C57BL/6J雌性小鼠，随机分为对照组（C组）、单纯照射组（R组）、染料木黄酮+照射组（Gen+R组）和氨磷汀 (Amifostine)+照射组（Ami+R组）(n=10)。除对照组外，其余各组小鼠接受8MV X线全胸照射(12.0±0.2) Gy。Gen+R组于照射开始前24、16、8 h皮下注射GEN 200 mg/kg，Ami+R组于照射开始前30 min皮下注射氨磷汀100 mg/kg，照射后每天1次连续给药4周。照射后4、8、12周，HE染色观察肺组织病理改变，ELISA方法测定血清中的TGF-β1的含量，比色法测定肺组织羟脯氨酸的含量。      结果        病理检测显示照射后时间越长，放射性肺损伤小鼠肺纤维化越重，染料木黄酮能减轻肺组织炎症及纤维化；照射后小鼠血清TGF-β1和肺组织羟脯氨酸的含量也呈递增趋势，染料木黄酮能降低TGF-β1和羟脯氨酸的含量(P<0.05)，尤其是照射前24 h给予染料木黄酮能达到与氨磷汀类似的疗效。      结论       染料木黄酮可减轻肺组织的纤维化，具有明显的辐射防护作用。
Abstract:
Objective       To evaluate the effect of genistein as a protecting agent on the radiation-induced pulmonary fibrosis in mice.       Methods        The thoraces of female 8-week-old C57BL/6J mice were exposed to either sham irradiation or single fraction of 8MV X ray radiation of (12.0±0.2) Gy. A total of 60 mice were divided as Sham (C group, n=10), radiation (R group, n=10), genistein+radiation (Gen+R group, n=30), amifostine+radiation (Ami+R group, n=10). The mice of Gen+R group were administered with genistein (200 mg/kg) in 8, 16 and 24 h respectively before irradiation (n=10) and then administered once per day until 4 weeks. While the mice of Ami+R group was injected subcutaneously by 100 mg/kg amifostine in 30 min before irradiation and in every day until 4 weeks. Mice were sacrificed at 4, 8, and 12 weeks post-irradiation. The lung tissues were removed and processed for definitive analysis, including hydroxypmline content and HE staining. ELISA was used to measure the serum level of transforming growth factor β1 (TGF-β1).       Results       Pathological study showed that pulmonary fibrosis became more severe along with the time after irradiation, and genistein attenuated lung inflammation and fibrosis. Radiation increased the expression of TGF-β1 and hydroxyprolin content in lung tissue in a time-dependent manner, and genistein reduced TGF-β1 expression and hydroxyprolin content (P<0.05).       Conclusion       Genistein attenuates the progress of radiation-induced lung fibrosis, and exerts significant effect on prevention against radiation.

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更新日期/Last Update: 2012-11-06