[1]高娟,康炜,陈俊霞,等.siRNA沉默整合素连接激酶对人膀胱癌细胞凋亡的影响[J].陆军军医大学学报(原第三军医大学学报),2012,34(21):2154-2157.
 Gao Juan,Kang Wei,Chen Junxia,et al.Integrin-linked kinase siRNA induces apoptosis in human bladder cancer BIU-87 cells[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(21):2154-2157.
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陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
34卷
期数:
2012年第21期
页码:
2154-2157
栏目:
论著
出版日期:
2012-11-15

文章信息/Info

Title:
Integrin-linked kinase siRNA induces apoptosis in human bladder cancer BIU-87 cells
作者:
高娟康炜陈俊霞朱军潘湘阳
西安医学院附属医院检验科;重庆医科大学基础医学院细胞生物学与遗传学教研室,分子医学与肿瘤研究中心
Author(s):
Gao Juan Kang Wei Chen Junxia Zhu Jun Pan Xiangyang
Department of Medical Laboratory, Affiliated Hospital of Xi’an Medical College, Xi’an, Shaanxi Province, 710077; Department of Cell Biology and Genetics, Molecule Medical and Tumor Research Center, College of Basic Medical Sciences, Chongqing Medical University, Chongqing, 400016, China
关键词:
整合素连接激酶膀胱癌凋亡
Keywords:
integrin linked kinase bladder cancer apoptosis
分类号:
R394.2; R730.23; R737.14
文献标志码:
A
摘要:
目的      探讨应用整合素连接激酶(integrin-linked kinase, ILK)特异性siRNA沉默ILK基因的表达对人膀胱癌细胞凋亡的影响。      方法      将构建好的针对ILK基因的1个特异性siRNA表达质粒和1个无同源性的阴性对照质粒,在脂质体介导下稳定转染人膀胱癌BIU-87细胞,并将实验分为BIU-87 si ILK、BIU-87 vector及BIU-87细胞3组,运用TUNEL凋亡检测试剂盒和流式细胞术检测BIU-87细胞的凋亡,进一步运用Western blot检测siRNA ILK对凋亡相关蛋白Caspase-3、Bax和Bcl-2的影响。      结果      TUNEL检测结果显示,BIU-87 si ILK组出现大量凋亡细胞,而BIU-87细胞组和BIU-87 vector组仅有少量凋亡细胞出现。流式细胞仪检测结果显示,BIU-87 si ILK组细胞大约有(75.70±2.00)%的凋亡细胞,而BIU-87 vector组和BIU-87细胞组仅有(0.88±0.10)%和(1.66±0.90)%的凋亡细胞,Western blot结果显示,在BIU-87 si ILK组中,Bcl-2的表达明显降低(P<0.05),而Bax和Caspase-3的表达明显升高(P<0.01)。       结论       siRNA ILK可能通过调节凋亡相关蛋白Bcl-2、Bax及Caspase-3的表达诱导膀胱癌BIU-87细胞凋亡的发生。
Abstract:
Objective      To determine the effects of integrin-linked kinase (ILK) siRNA on the apoptosis of human bladder cancer BIU-87 cells.       Methods      Two siRNA vectors specific to ILK gene and one non-homologous negative control vector were designed and constructed, then stably transfected into bladder cancer BIU-87 cells via Lipofectamine 2000, and were named as BIU-87 si ILK and BIU-87 vector. Cell apoptosis was assessed by TUNEL kit and flow cytometry. The expression of Caspase-3, Bax and Bcl-2 were assessed by Western blotting.       Results       The number of TUNEL-positive cells was significantly increased in the BIU-87 si ILK cells when compared with BIU-87 cells and BIU-87 vector cells. Flow cytometry showed that (75.70±2.00)% of counted cells became apoptotic in BIU-87 si ILK cells, however, about only (0.88±0.10)% and (1.66±0.90)% of counted cell became apoptotic in BIU-87 vector cells and BIU-87 cells groups respectively. The expression of Bcl-2 was significantly reduced in BIU-87 si ILK cells compared with BIU-87 vector and BIU-87 cells(P<0.05), and that of Caspase-3 and Bax protein was stably increased (P<0.01).       Conclusion      The siRNA ILK induces apoptosis in bladder cancer BIU-87 cells by regulating apoptosis-related proteins Caspase-3, Bax and Bcl-2.

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更新日期/Last Update: 2012-11-06