[1]关雪晶,吴宏,何晓莉,等.当归多糖对辐射损伤小鼠骨髓基质细胞的影响[J].第三军医大学学报,2013,35(08):779-783.
 Guan Xuejing,Wu Hong,He Xiaoli,et al.Effects of Angelica polysaccharides on bone marrow stromal cells in radiation injured mice[J].J Third Mil Med Univ,2013,35(08):779-783.
点击复制

当归多糖对辐射损伤小鼠骨髓基质细胞的影响(/HTML )
分享到:

《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
35卷
期数:
2013年第08期
页码:
779-783
栏目:
论著
出版日期:
2013-04-30

文章信息/Info

Title:
Effects of Angelica polysaccharides on bone marrow stromal cells in radiation injured mice
作者:
关雪晶吴宏何晓莉姜蓉
重庆医科大学基础医学院干细胞与组织工程研究室,组织胚胎学教研室
Author(s):
Guan Xuejing Wu Hong He Xiaoli Jiang Rong
Laboratory of Stem Cells and Tissue Engineering, Department of Histology and Embryology, College of Basic Medical Sciences, Chongqing Medical University, Chongqing, 400016, China
关键词:
当归多糖辐射损伤骨髓基质细胞凋亡率黏附分子
Keywords:
Angelica polysaccharides radiation injury bone marrow stromal cell apoptotic ratio adhesion molecule
分类号:
R285.5;R331.22;R818.74
文献标志码:
A
摘要:
目的   研究当归多糖(Angelica polysaccharides,APS)对辐射损伤小鼠骨髓基质细胞(bone marrow stromal cell,BMSC)的保护作用。   方法   BALB/c小鼠按随机数字表法分为10组,每组24只,共计240只。正常组不作任何处理,其余9组经X射线照射后分别给予生理盐水(NS)、2 mg/kg APS和8 mg/kg APS,给药至第7、14、21天计数小鼠外周血白细胞(WBC)、红细胞(RBC)、血小板(PLT)及骨髓单个核细胞(BMNC);观察BMSC生长达80%贴壁所需时间及形成的成纤维细胞集落(CFU-F)数量;流式细胞术检测BMSC细胞周期、凋亡率及表面黏附分子CD54、CD106的表达水平。   结果   与正常组比较,NS组外周血WBC、RBC、PLT及BMNC计数明显减少(P<0.05);BMSC达80%贴壁所需时间明显延长,CFU-F数明显减少(P<0.05);G0/G1期细胞比例显著增加,S期细胞比例显著降低(P<0.05);BMSC细胞凋亡率明显增高(P<0.05);CD54、CD106表达明显降低(P<0.05)。2 mg/kg APS、8 mg/kg APS组与NS组相比,外周血各指标、BMNC数及CFU-F数明显增加(P<0.05);BMSC达80%融合时间显著缩短(P<0.05);G0/G1期比例明显降低,S期比例显著增加(P<0.05);凋亡率显著降低(P<0.05);CD54、CD106表达明显增高(P<0.05)。第21天8 mg/kg APS组各指标均恢复正常。   结论   APS可能是通过提高BMSC贴壁及增殖能力,加速BMSC由G0/G1期向S期转换,降低BMSC凋亡率,上调CD54、CD106表达来促进辐射损伤后小鼠造血功能的恢复。
Abstract:
Objective   To investigate the radioprotective effect of Angelica polysaccharides (APS) on bone marrow stromal cells (BMSCs) in radiation injured mice.    Methods   Two hundred and forty BALB/c mice were randomly divided into 10 groups, including a normal group, three NS groups, three 2 mg/kg APS groups and three 8 mg/kg APS groups, and there were 24 mice in each group. All the mice except for those of the normal group underwent X-ray radiation, and the radiated mice were injected with NS, 2 mg/kg APS and 8 mg/kg APS for 7, 13 and 21 d, respectively. The numbers of white blood cells (WBC), red blood cells (RBC), platelets and bone marrow mononuclear cells (BMNCs) in peripheral blood were recorded by cell counting after treatment. The time for BMSC growing to 80% adherence and the number of fibroblast colony-forming unit (CFU-F) were observed. Cell cycle, apoptotic ratio and the expression of CD54 and CD106 on BMSC were detected by flow cytometry.    Results   Compared with the normal group, the cell counts of WBC, RBC, platelets and BMNC in peripheral blood of the NS groups decreased significantly (P<0.05), the time for BMSCs growing to 80% adherence prolonged significantly (P<0.05), and the number of CFU-F was significantly decreased (P<0.05). The results of flow cytometry showed that compared with the normal group, the percentage of BMSCs was significantly increased at G0/G1 phase but significantly declined at S phase (P<0.05), the apoptotic ratio was significantly increased (P<0.05), and the expression of CD54 and CD106 on BMSC was decreased significantly (P<0.05). Compared with the NS groups, the results of the 2 mg/kg APS groups and 8 mg/kg APS groups showed that the numbers of peripheral blood cells, BMNCs and CFU-F significantly increased (P<0.05), the time for BMSC growing to 80% adherence was significantly decreased, the percentage of BMSCs was significantly decreased at G0/G1 phase but significantly increased at S phase (P<0.05), the apoptotic ratio was significantly decreased (P<0.05), and the expression of CD54, CD106 on BMSC was significantly increased (P<0.05). The indicators of the 8 mg/kg APS groups returned to normal levels on the 21st day.    Conclusion   For BMSCs, APS can promote adherence and proliferation, accelerate transition from G0/G1 phase to S phase, decline apoptotic ratio, and up-regulate the expression of CD54 and CD106, so as to accelerate the hematopoietic recovery of radiation injured mice.

相似文献/References:

[1]胡晶,冯敏,杨慧,等.当归多糖动员的造血干/祖细胞移植重建小鼠造血功能的研究[J].第三军医大学学报,2007,29(23):2236.
 HU Jing,FENG Min,YANG Hui,et al.Hematopoiesis reconstruction in mice of hematopoietic aplasia by transplanting hematopoietic stem/progenitor cells mobilized by Angelica polysaccharides[J].J Third Mil Med Univ,2007,29(08):2236.
[2]周永,糜漫天,朱俊东,等.三羟异黄酮对小鼠辐射损伤后造血功能的影响[J].第三军医大学学报,2007,29(10):866.
 ZHOU Yong,MI Man-tian,ZHU Jun-dong,et al.Effect of genistein on hematopoiesis in irradiated mice[J].J Third Mil Med Univ,2007,29(08):866.
[3]王富友,高京生.低氧对大鼠小肠上皮细胞辐射损伤修复的影响[J].第三军医大学学报,2005,27(08):700.
[4]周永,糜漫天,杨镇洲.三羟异黄酮对辐射损伤小鼠骨髓基质细胞的保护作用[J].第三军医大学学报,2005,27(06):471.
[5]李招权,万莹铧,李军,等.外源性CCN1促进辐射损伤L929细胞的生长和迁移[J].第三军医大学学报,2010,32(11):1127.
 Li Zhaoquan,Wan Yinghua,Li Jun,et al.Exogenous CCN1 promotes proliferation and migration of radiation-injured L929 cells[J].J Third Mil Med Univ,2010,32(08):1127.
[6]卿轶,董志.当归多糖对大鼠肝性脑病的预防作用[J].第三军医大学学报,2008,30(23):2187.
 QING Yi,DONG Zhi.Angelica sinensis polysaccharides prevents rat from hepatic encephalopathy[J].J Third Mil Med Univ,2008,30(08):2187.
[7]舒崇湘,程天民.康复新对单纯皮肤创面和合并全身放射损伤时的促愈作用[J].第三军医大学学报,1999,21(03):0.[doi:10.16016/j.1000-5404.1999.03.005 ]
 Shu Chongxiang,Cheng Tianmin.[J].J Third Mil Med Univ,1999,21(08):0.[doi:10.16016/j.1000-5404.1999.03.005 ]
[8]张舟,陈芳,曾东风,等.TMP线性二联体多肽的筛选及其对辐射损伤小鼠血小板减少症的救治作用[J].第三军医大学学报,2013,35(19):2005.
 Zhang Zhou,Chen Fang,Zeng Dongfeng,et al.Screening for dimmer of thrombopoietin mimetic peptide with potent thrombo-cytopoietic effect and its effect on radiation-induced thrombocytopenia in mice[J].J Third Mil Med Univ,2013,35(08):2005.
[9]宫立众,孙士红,程天民,等.小鼠全身照射后血中造血祖细胞水平变化[J].第三军医大学学报,2004,26(04):0.[doi:10.16016/j.1000-5404.2004.04.034 ]
[10]胡川闽,粟永萍,程天民,等.小鼠轻型肠型放射病肠上皮细胞差异表达基因的筛选研究[J].第三军医大学学报,2002,24(06):0.[doi:10.16016/j.1000-5404.2002.06.033 ]
 HU Chuan min,SU Yong ping,CHENG Tian min,et al.[J].J Third Mil Med Univ,2002,24(08):0.[doi:10.16016/j.1000-5404.2002.06.033 ]

更新日期/Last Update: 2013-04-22