[1]刘冬,熊仁平,陈星云,等.ROCK1在地塞米松抑制中性粒细胞释放和单核细胞黏附中的作用[J].第三军医大学学报,2012,34(18):1814-1817.
 Liu Dong,Xiong Renping,Chen Xingyun,et al.Role of ROCK1 in dexamethasone-inhibited neutrophil release and monocyte adhesion in vitro[J].J Third Mil Med Univ,2012,34(18):1814-1817.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
34卷
期数:
2012年第18期
页码:
1814-1817
栏目:
论著
出版日期:
2012-09-30

文章信息/Info

Title:
Role of ROCK1 in dexamethasone-inhibited neutrophil release and monocyte adhesion in vitro
作者:
刘冬熊仁平陈星云李平宁亚蕾彭艳赵艳杨楠周元国
第三军医大学大坪医院野战外科研究所分子生物学中心,创伤烧伤及复合伤国家重点实验室
Author(s):
Liu Dong Xiong Renping Chen Xingyun Li Ping Ning Yalei Peng Yan Zhao Yan Yang Nan Zhou Yuanguo
State Key Laboratory of Trauma, Burns, and Combined Injury, Molecular Biology Center, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing, 400042, China
关键词:
地塞米松非基因组效应ROCK1
Keywords:
dexamethasone non-genomic effectsROCK1
分类号:
R392.12;R966;R977.11
文献标志码:
A
摘要:
目的      探讨ROCK1在地塞米松(dexamethasone,Dex)抑制中性粒细胞释放和单核细胞黏附中的作用。      方法      检测地塞米松和法舒地尔(Fasudil,Fas)抑制佛波脂(PMA)诱导的中性粒细胞释放超氧化物阴离子(O2-)和髓过氧化物酶(myeloperoxidase ,MPO)以及U937单核细胞黏附的程度,并检测ROCK1和RhoA的表达和活性改变。此外观察糖皮质激素受体拮抗剂——米非司酮(mifepristone,M)和蛋白质合成抑制剂——放线菌酮(cycloheximide,C)对地塞米松这些作用的影响。实验分组:对照组、PMA组、PMA+Dex组、PMA+Dex+M组、PMA+Dex+C组和PMA+Fas组。      结果      地塞米松可以显著抑制中性粒细胞释放O2- 和MPO[(1.25±0.10) vs (1.95±0.10);(1.07±0.06) vs (1.75±0.08),P<0.05],而法舒地尔对其无明显抑制作用(P>0.05)。地塞米松和法舒地尔均可抑制U937细胞黏附[(0.09±0.01) vs (0.08±0.01) vs (0.28±0.06), P<0.05]。米非司酮和放线菌酮并不能影响地塞米松的这些作用(P>0.05)。      结论      地塞米松以非ROCK1依赖方式抑制中性粒细胞释放;以ROCK1依赖方式通过非基因组效应抑制U937单核细胞的黏附。
Abstract:
Objective      To determine the role of ROCK1 in the inhibitory effect of dexamethasone (Dex) on neutrophil release and monocyte adhesion.       Methods      The inhibitory effect of Dex and Fasudil (Fas) on the release superoxide anion (O2-) and myeloperoxidase (MPO) in neutrophil (PMN) after treatment of PMA, and the adhesion ability of U937 cells to human umbilical vein endothelial cells (HUVEC) after the stimulation as same as above mentioned was also determined. The expression and activity of ROCK1 and RhoA were also determined. Furthermore, the effect of glucocorticoid receptor antagonists (mifepristone, M) and protein synthesis inhibitors (cycloheximide, C) on dexamethasone were also evaluated. Cell samples were divided into control group, PMA group, PMA+Dex group, PMA+Dex+M group, PMA+Dex+C group and PMA+Fas group.       Results      Dex inhibited the release O2- and MPO of PMA-stimulated PMN (1.25±0.10 vs 1.95±0.10, 1.07±0.06 vs 1.75±0.08, P<0.05), but Fasudil had no this function (P>0.05). Dexamethasone and Fasudil inhibited adhesion ability of U937 cells significantly(0.09±0.01 and 0.08±0.01 vs 0.28±0.06, P<0.05). Mifepristone and cycloheximide did not interfere with the effect of Dex.       Conclusion      Dex inhibits the release of neutrophil via non-ROCK1 dependent pathway, and discourages U937 monocyte adhesion in ROCK1 dependent pathway via non-genomic effects.

参考文献/References:

刘冬, 熊仁平, 陈星云, 等. ROCK1在地塞米松抑制中性粒细胞释放和单核细胞黏附中的作用[J].第三军医大学学报,2012,34(18):1814-1817.

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更新日期/Last Update: 2012-09-18