[1]谢湘竹,赵水平,于碧莲,等.高密度脂蛋白对氧化型低密度脂蛋白刺激下脂肪细胞TNF-α表达的影响[J].第三军医大学学报,2012,34(18):1822-1825.
 Xie Xiangzhu,Zhao Shuiping,Yu Bilian,et al.High density lipoprotein suppresses oxidized low density lipoprotein-induced tumor necrosis factor-α in 3T3-L1 adipocytes[J].J Third Mil Med Univ,2012,34(18):1822-1825.
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高密度脂蛋白对氧化型低密度脂蛋白刺激下脂肪细胞TNF-α表达的影响(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
34卷
期数:
2012年第18期
页码:
1822-1825
栏目:
论著
出版日期:
2012-09-30

文章信息/Info

Title:
High density lipoprotein suppresses oxidized low density lipoprotein-induced tumor necrosis factor-α in 3T3-L1 adipocytes
作者:
谢湘竹赵水平于碧莲钟巧青
解放军总医院南楼心血管一科;中南大学湘雅二医院心内科
Author(s):
Xie Xiangzhu Zhao Shuiping Yu Bilian Zhong Qiaoqing
First Department of Cardiology in South Building, General Hospital of PLA, Beijing, 100853; Department of Cardiology, Second Xiangya Hospital, Central South University, Changsha, Hunan Province, 410011, China
关键词:
脂肪细胞氧化型低密度脂蛋白高密度脂蛋白肿瘤坏死因子-α核因子κB蛋白激酶A
Keywords:
adipocytes oxidized low density lipoprotein high density lipoprotein tumor necrosis factor-α nuclear factor-kappa B protein kinase A
分类号:
Q513.5;R329.26;R394.3
文献标志码:
A
摘要:
目的      观察高密度脂蛋白(high density lipoprotein, HDL)对氧化型低密度脂蛋白(oxidized low density lipoprotein,oxLDL)刺激下3T3-L1脂肪细胞肿瘤坏死因子-α(tumor necrosis factor-α, TNF-α)mRNA表达的影响,并探讨其可能的作用机制。      方法      3T3-L1脂肪细胞促分化成熟后,oxLDL刺激脂肪细胞,给予不同浓度的HDL(10~100 μg/ml),及H-89(10 μmol/L)+HDL(100 μg/ml)干预,收集细胞,测定脂肪细胞TNF-α mRNA表达水平,IκB蛋白浓度及核因子-κB(NF-κB)活性。      结果      OxLDL刺激使3T3-L1脂肪细胞TNF-α mRNA表达及NF-κB活性明显增强。HDL浓度依赖性抑制TNF-α mRNA表达、NF-κB活化和IκB降解。与oxLDL刺激组比较,100 μg/ml HDL使TNF-α mRNA表达降低64.5%,NF-κB活性减少49%,并明显增加IκB蛋白水平。HDL的这些抗炎效应能被蛋白激酶A(PKA)抑制剂H-89部分抑制。      结论      HDL能抑制oxLDL诱导的3T3-L1脂肪细胞TNF-α mRNA表达,PKA-IκB-NF-κB信号通路是其中作用途径之一,该效应不需要HDL与oxLDL的直接接触作用。
Abstract:
Objective      To determine the effect of high density lipoprotein (HDL) on the mRNA expression of tumor necrosis factor-α (TNF-α) in oxidized low density lipoprotein (oxLDL)-stimulated 3T3-L1 adipocytes.       Methods      Fully differentiated 3T3-L1 adipocytes were incubated in the medium containing various concentrations of HDL (10 to 100 μg/ml) with oxLDL (50 μg/ml) stimulation, with/without H-89 (10 μmol/L) preincubation. TNF-α mRNA expression was determined by RT-PCR, NF-κB activity was measured by ELISA, and IκB protein level was tested by Western blotting.       Results      OxLDL stimulation induced a significant increase of TNF-α mRNA and NF-κB activity in 3T3-L1 adipocytes. HDL inhibited TNF-α mRNA expression, NF-κB activation and IκB degradation in a dose-dependent manner. Compared with oxLDL stimulation, HDL at 100 μg/ml resulted in a decrease of TNF-α mRNA expression by 64.5% and NF-κB activation by 49%, and an increase of IκB protein. The anti-inflammatory effect of HDL was abolished by PKA inhibitor H-89 partially.       Conclusion      HDL could suppress TNF-α mRNA expression in oxLDL-stimulated 3T3-L1 adipocytes through PKA- IκBα-NF-κB signaling pathway, without any direct contact between HDL and oxLDL.

参考文献/References:

谢湘竹, 赵水平, 于碧莲, 等. 高密度脂蛋白对氧化型低密度脂蛋白刺激下脂肪细胞TNF-α表达的影响[J].第三军医大学学报,2012,34(18):1822-1825.

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更新日期/Last Update: 2012-09-18