[1]彭辉,陈维贤,常琳,等.HBV感染相关的慢加急性肝衰竭患者外周血中TCR γδT细胞促炎细胞因子的产生及与临床指标的相关性分析[J].陆军军医大学学报(原第三军医大学学报),2012,34(17):1733-1736.
　Peng Hui,Chen Weixian,Chang Lin,et al.Proinflammatory cytokine production of TCR γδT cells in peripheral blood of patients with HBV-associated acute-on-chronic liver failure, and correlation analysis between cell proportion and clinical parameters[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(17):1733-1736.
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HBV感染相关的慢加急性肝衰竭患者外周血中TCR γδT细胞促炎细胞因子的产生及与临床指标的相关性分析(/HTML )
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陆军军医大学学报（原第三军医大学学报）[ISSN:1000-5404/CN:51-1095/R]

卷:
34卷
期数:
2012年第17期
页码:
1733-1736
栏目:
论著
出版日期:
2012-09-15

文章信息/Info

Title:
Proinflammatory cytokine production of TCR γδT cells in peripheral blood of patients with HBV-associated acute-on-chronic liver failure, and correlation analysis between cell proportion and clinical parameters
作者:
彭辉; 陈维贤; 常琳; 陈敏
重庆医科大学：附属第二医院检验，病毒性肝炎研究所，感染性疾病分子生物学省部共建教育部重点实验室
Author(s):
Peng Hui;  Chen Weixian;  Chang Lin;  Chen Min
Department of Laboratory Medicine, Second Affiliated Hospital, Chongqing Medical University; Key Laboratory of Molecular Biology for Infectious Diseases cofounded by Chongqing and Ministry of Education, Institute for Viral Hepatitis, Chongqing Medical University, Chongqing, 400010, China
关键词:
γδT细胞; 慢加急性肝衰竭; 乙型肝炎病毒; 炎性因子
Keywords:
γδT;  acute-on-chronic liver failure;  hepatitis B virus;  inflammatory cytokine
分类号:
R373.21; R392.32; R575.302
文献标志码:
A
摘要:
目的      探讨HBV感染相关的慢加急性肝衰竭患者(HBV-associated acute-on-chronic liver failure，HBV-ACLF）外周血中TCRγ δT（γδT）细胞的比例及产生IFN-γ、TNF-α及IL-17等促炎细胞因子的能力，分析它们与临床指标间的相关性。      方法      入选26例HBV-ACLF患者、40例慢性HBV感染患者（CHBV）、25例健康对照者，用流式细胞仪方法检测外周血中γδT细胞的频率及产生炎性细胞因子IFN-γ、TNF-α、IL-17细胞的比例，采用SPSS 13.0统计软件分析各组间各细胞比例的差异及细胞比例与临床指标的相关性。      结果      HBV-ACLF患者外周血中γδT细胞占总T细胞的比例的中位值为4.8%，低于CHB患者（10.7%）与健康对照者（10.3%），差异有统计学意义（P<0.01）。在体外经刺激后，IFN-γ阳性的γδT细胞在HBV-ACLF组的比例中位值为73.9%、CHBV组为70.9%，高于健康对照组（45.3%，P<0.01）；HBV-ACLF患者中TNF-α阳性的γδT细胞中位值为26.7%，显著高于CHBV患者（11.5%）及健康对照组（11.7%，P<0.01）；产生IL-17 因子的γδT在HBV-ACLF组中中位值为0.7%，高于CHBV组(0.3%)及健康对照组(0.3%，P<0.01）。经Spearman Correlation相关性分析，HBV-ACLF组中TNF-α+ γδT 细胞的比例与ALT或AST水平呈负相关。      结论      虽然HBV-ACLF患者外周血中总的γδT细胞比例降低，但产生炎性细胞因子IFN-γ、TNF-α、IL-17的能力显著增强，可能参与了HBV-ACLF发病过程中炎症因子急剧升高的过程。
Abstract:
Objective       To explore the proportion of TCR γδT (γδT) cells and the cytokine production ability of the γδT cells in peripheral blood of patients with hepatitis B virus-associated acute-on-chronic liver failure (HBV-ACLF), and to analyze the correlation between the proportion of cells and the clinical parameters.       Methods       Twenty-six patients with HBV-ACLF (HBV-ACLF group), 40 patients with chronic HBV infection (CHBV group), and 25 healthy controls (HC group) were enrolled in this study. The proportion of γδT cells in peripheral blood of the subjects and the proportion of IFN-γ, TNF-α, or IL-17 producing γδT cells were determined by flow cytometry. The differences of the proportion of cytokine-producing γδT cells among the three groups and the correlation of the proportion with clinical parameters were statistically analyzed by SPSS software.       Results       The proportion of γδT cells in peripheral blood of the HBV-ACLF group (4.8% as median) was significantly lower than that of the CHBV group (10.7% as median) and HC group (10.3% as median) (P<0.01). After stimulation, the proportion of IFN-γ+ γδT cells in the HBV-ACLF group (73.9% as median) and CHBV group (70.9% as median) was significantly higher than that in the HC group (45.3% as median) (P<0.01). The proportions of TNF-α+ γδT cells and IL-17+ γδT cells (26.7% and 0.7% as median) were significantly higher in the HBV-ACLF group than in the CHBV group (11.5% and 0.3%) and HC group (11.7% and 0.3%) (P<0.01). Moreover, the proportion of TNF-α+γδT cells was negatively correlated with the levels of ALT and AST.       Conclusion       Although the proportion of total γδT cells in peripheral blood of HBV-ACLF patients decreases, the ability of the γδT cells in producing IFN-γ, TNF-α and IL-17 is enhanced, which may be involved in the acute systemic inflammatory responses in HBV-ACLF development.

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更新日期/Last Update: 2012-08-31