[1]何迎盈,刘娜娜,罗治彬,等.阿苯达唑抑制P-糖蛋白表达对耐顺铂卵巢癌细胞耐药性的影响[J].陆军军医大学学报(原第三军医大学学报),2012,34(17):1762-1765.
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阿苯达唑抑制P-糖蛋白表达对耐顺铂卵巢癌细胞耐药性的影响(/HTML )
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陆军军医大学学报（原第三军医大学学报）[ISSN:1000-5404/CN:51-1095/R]

卷:
34卷
期数:
2012年第17期
页码:
1762-1765
栏目:
论著
出版日期:
2012-09-15

文章信息/Info

Title:
Albendazole reverses chemoresistance of cisplatin-resistant ovarian cancer cells by inhibiting P-glycoprotein expression
作者:
何迎盈; 刘娜娜; 罗治彬; 李少林
重庆医科大学基础医学院核医学教研室；重庆市合川区人民医院肿瘤血液科
Author(s):
He Yingying;  Liu Nana;  Luo Zhibin;  Li Shaolin
Department of Nuclear Medicine, College of Basic Medical Sciences, Chongqing Medical University, Chongqing, 400016; Department of Hematology, Peoples’ Hospital of Hechuan District, Chongqing, 401520, China
关键词:
阿苯达唑; P-糖蛋白; 耐药性
Keywords:
albendazole;  P-glycoprotein;  chemoresistance
分类号:
R965; R978.6; R979.1
文献标志码:
A
摘要:
目的      探讨阿苯达唑（albendazole，ABZ）通过抑制人耐顺铂卵巢癌SKOV-3/DDP细胞P-糖蛋白（P-glycoprotein，P-gp）的表达对细胞耐药性的影响。      方法        在体外，用溴化二甲噻唑二苯四氨唑（MTT）比色法检测ABZ对SKOV-3/DDP细胞增殖的影响，求得抑制率为0%、25%、50%、75%所对应的ABZ浓度，并按抑制率将实验分为对照组，25%抑制浓度（IC25）组、半数抑制浓度（IC50）组和75%抑制浓度（IC75）组，每组给予相应浓度的ABZ处理，并按作用时间再将每组分为12、24、36 h亚组。分别用流式细胞仪检测各组细胞表面P-gp的表达。将不同浓度的顺铂（cisplatin，DDP）、表阿霉素（epirubicin，EPI）、5-氟尿嘧啶（5-fluorouracil，5-Fu）按照分组，联合和序贯ABZ处理细胞后，采用MTT比色法检测SKOV-3/DDP细胞体外增殖情况。      结果        ABZ作用SKOV3/DDP细胞后使P-gp表达下降，呈浓度和时间依赖性。联合和序贯ABZ均能降低DDP、EPI和5-Fu对SKOV-3/DDP细胞的作用浓度，有浓度和时间依赖性，且联合作用优于序贯作用。      结论       在体外ABZ通过抑制SKOV-3/DDP细胞P-gp表达提高细胞对DDP、EPI、5-Fu的敏感性，并能逆转细胞的耐药性。
Abstract:
Objective      To investigate the effect of albendazole (ABZ) on the chemoresistance of cisplatin-resistant human ovarian cancer SKOV-3/DDP cells by inhibiting P-glycoprotein (P-gp) expression.       Method      The effect of ABZ on the proliferation of SKOV-3/DDP cells was detected by methyl thiazolyl tetrazolium (MTT) colorimetry. According to the inhibition concentrations (IC) of 0%, 25%, 50% and 75%, the cells were then divided into 4 groups including a control group, an IC25 group, an IC50 group and an IC75 group. Moreover, each group was divided into three subgroups (12, 24 and 36 h) based on the ABZ treatment time. P-gp expression was determined by flow cytometry. The proliferation of SKOV-3/DDP cells was detected by MTT colorimetry after the treatment with ABZ combined and sequential with cisplatin (DDP), epirubicin (EPI) and 5-fluorouracil (5-Fu).       Results      The expression of P-gp was decreased by ABZ in a dose- and time-dependent manner. Both combined and sequential with ABZ could decrease the effective concentrations of DDP, EPI and 5-Fu to SKOV-3/DDP cells in a dose- and time-dependent manner, and the effect of combination was better than that of sequential treatment.       Conclusion      Through down-regulating the expression of P-gp in SKOV-3/DDP cells, ABZ can increase the sensitivity of SKOV-3/DDP cell to DDP, EPI and 5-Fu, and reverse its chemoresistance.

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更新日期/Last Update: 2012-08-31