[1]万萍,钟兵,邹庆华,等.类风湿关节炎早期诊断指标的筛选及鉴定[J].第三军医大学学报,2012,34(15):1564-1567.
 Wan Ping,Zhong Bing,Zou Qinhua,et al.Manganese superoxide dismutase: an early diagnostic marker for rheumatoid arthritis[J].J Third Mil Med Univ,2012,34(15):1564-1567.
点击复制

类风湿关节炎早期诊断指标的筛选及鉴定(/HTML )
分享到:

《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
34卷
期数:
2012年第15期
页码:
1564-1567
栏目:
论著
出版日期:
2012-08-15

文章信息/Info

Title:
Manganese superoxide dismutase: an early diagnostic marker for rheumatoid arthritis
作者:
万萍钟兵邹庆华方勇飞
第三军医大学西南医院中西医结合科
Author(s):
Wan Ping Zhong Bing Zou Qinhua Fang Yongfei
Department of Chinese and Western Integrated Medicine, Southwest Hospital, Third Military Medical University. Chongqing, 400038, China
关键词:
类风湿关节炎自身抗体锰超氧化物歧化酶早期诊断
Keywords:
rheumatoid arthritis autoantibody manganese superoxide dismutase early diagnosis
分类号:
R446; R593.22
文献标志码:
A
摘要:
目的      寻找类风湿关节炎的早期诊断指标。      方法        采用双向电泳、MALDI-TOF/TOF质谱分析等方法从类风湿关节炎滑膜组织中筛选并鉴定特异性自身抗原,进一步建立该抗体的ELISA检测方法,测定30例早期类风湿关节炎患者及30例健康对照者血清中的抗体水平,并分析抗体水平与疾病活动度的相关性。      结果        早期类风湿关节炎血清中筛选并鉴定出特异性的锰超氧化物歧化酶抗体。ELISA法检测早期RA血清中锰超氧化物歧化酶抗体D(450)值均值(1.72±0.35)明显高于正常人血清(0.95±0.34)(P<0.0001)。在早期RA患者血清中锰超氧化物歧化酶抗体阳性率为63.3%,且该抗体水平与RF、CCP、CRP呈明显正相关。      结论        锰超氧化物歧化酶抗体可能作为类风湿关节炎早期诊断与疾病活动性预测的指标之一。
Abstract:
Objective       To investigate an early diagnostic marker for rheumatoid arthritis.       Methods      Two-dimensional electrophoresis and MALDI-TOF/TOF mass spectrometry were employed to screen and identify the specific autoantigen in the fresh synovial tissues from 2 patients with identified rheumatoid arthritis. ELISA was used to measure the antibodies in the 30 serum samples from patients with early rheumatoid arthritis and the 30 serum samples from healthy donors. The correlation between antibody levels and disease activity was analyzed.       Results        Manganese superoxide dismutase antibody was specific presence in the serum of early rheumatoid arthritis. The mean D(450) value of manganese superoxide dismutase antibody was 1.72±0.35 in early RA serum, significantly higher than normal serum (0.95±0.34, P<0.0001). The levels of manganese superoxide dismutase antibody was positively correlated with levels of RF, CCP and CRP in patients with early RA. The positive rate for these serum samples was 63.3% to manganese superoxide dismutase antibody RA.       Conclusion        Manganese superoxide dismutase antibodies might be used as a biomarker for early diagnosis of rheumatoid arthritis and for prediction of disease activity.

参考文献/References:

万萍, 钟兵, 邹庆华, 等. 类风湿关节炎早期诊断指标的筛选及鉴定[J].第三军医大学学报,2012,34(15):1564-1567.

相似文献/References:

[1]柏干苹,周丽娜,贺伟峰,等.类风湿关节炎患者滑膜成纤维细胞的差异蛋白质谱分析[J].第三军医大学学报,2008,30(02):166.
 BO Gan-ping,ZHOU Li-na,HE Wei-feng,et al.Differential proteome of human synovial fibroblasts from rheumatoid arthritis patients[J].J Third Mil Med Univ,2008,30(15):166.
[2]吴建红,魏洪兵,姚朝群.来氟米特治疗类风湿关节炎的临床研究[J].第三军医大学学报,2007,29(03):274.
[3]柏干苹,周丽娜,贺伟峰,等.双向电泳-质谱法寻找类风湿关节炎疾病相关蛋白[J].第三军医大学学报,2006,28(07):726.
[4]王雯,陈森洲,王险峰,等.HIF-1α、VEGF在CIA大鼠模型中的表达与意义[J].第三军医大学学报,2010,32(06):563.
 Wang Wen,Chen Senzou,Wang Xianfen,et al.Expression and significance of HIF-1α and VEGF in rats with collagen Ⅱ-induced arthritis[J].J Third Mil Med Univ,2010,32(15):563.
[5]夏璇,肖敬,黄清春.类风湿关节炎合并膜性肾病1例[J].第三军医大学学报,2010,32(17):1907.
[6]李景怡,方勇飞,邹丽云,等.Th1/Th2细胞因子谱在类风湿关节炎患者外周血和关节积液中的表达及意义[J].第三军医大学学报,2010,32(18):1921.
 Li Jingyi,Fang Yongfei,Zou Liyun,et al.Th1/Th2 cytokines’ profile in peripheral blood and synovial fluid of patients with rheumatoid arthritis[J].J Third Mil Med Univ,2010,32(15):1921.
[7]尉世同,孙永华,邹巧菲,等.双醋瑞因联合甲氨喋呤治疗类风湿关节炎的临床研究[J].第三军医大学学报,2010,32(19):2112.
 Wei Shitong,Sun Yonghua,Zou Qiaofei,et al.Combined diacerein and methotrexate in treatment of rheumatoid arthritis: report of 120 cases[J].J Third Mil Med Univ,2010,32(15):2112.
[8]刘青松,朱兴春,邢艳,等.丹参诱导类风湿关节炎患者成纤维样滑膜细胞中Fas/FasL的表达[J].第三军医大学学报,2010,32(22):2452.
[9]李静,柏干苹,王勇,等.401例类风湿关节炎患者中医证候要素分析[J].第三军医大学学报,2009,31(21):2103.
 LI Jing,BAI Gan-ping,WANG Yong,et al.Analysis on traditional Chinese medicine syndroms of 401 rheumatoid arthritis patients[J].J Third Mil Med Univ,2009,31(15):2103.
[10]肖敬,尹智功,蒋耀平,等.类风湿关节炎合并强直性脊柱炎1例[J].第三军医大学学报,2009,31(15):1494.

更新日期/Last Update: 2012-07-27