[1]罗静,叶兴伟,蒋文,等.阿糖胞苷诱导人MDS细胞株MUTZ-8细胞凋亡过程中活性氧水平的变化[J].第三军医大学学报,2012,34(15):1492-1495.
 Luo Jing,Ye Xingwei,Jiang Wen,et al.Alteration of reactive oxygen species in cytarabine-induced apoptosis process in MDS cell line MUTZ-8[J].J Third Mil Med Univ,2012,34(15):1492-1495.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
34卷
期数:
2012年第15期
页码:
1492-1495
栏目:
论著
出版日期:
2012-08-15

文章信息/Info

Title:
Alteration of reactive oxygen species in cytarabine-induced apoptosis process in MDS cell line MUTZ-8
作者:
罗静叶兴伟蒋文周洪静杨泽松刘林王利
重庆医科大学附属第一医院血液科;重庆医科大学附属第一医院第一分院超声科
Author(s):
Luo Jing Ye Xingwei Jiang Wen Zhou Hongjing Yang Zesong Liu Lin Wang Li
Department of Hematology, First Affiliated Hospital, Chongqing Medical University, Chongqing, 400016; Department of Ultrasonography, First Branch of First Affiliated Hospital, Chongqing Medical University, Chongqing, 400015, China
关键词:
细胞凋亡阿糖胞苷MUTZ-8骨髓增生异常综合征活性氧
Keywords:
apoptosis cytarabine MUTZ-8 cells myelodysplastic syndrome reactive oxygen species
分类号:
R73-361;R737.3;R979.12
文献标志码:
A
摘要:
目的      探讨阿糖胞苷诱导人MDS细胞株MUTZ-8细胞凋亡的可能机制。      方法       将细胞分为以下5组:阴性对照组、0.1 μmol/L Ara-c组、1 μmol/L Ara-c组、10 μmol/L Ara-c组、10 μmol/L Ara-c+62.5 μmol/L ALA组。用MTT法测定阿糖胞苷对各组细胞抑制率的影响,用Annexin V-FITC/PI 双染法及Hoechst33258荧光染色观察阿糖胞苷对各组细胞凋亡的影响,并用流式细胞仪检测各组细胞线粒体膜电位的变化。运用二氢二氯荧光素(DCFH-DA)为细胞内活性氧探针,通过荧光显微镜及流式细胞仪检测各组细胞内活性氧水平的变化。      结果       MTT结果提示,阿糖胞苷处理组细胞增殖抑制率高于阴性对照组,二者相比差异具有统计学意义(P<0.05)。流式细胞仪结果提示,不同浓度的阿糖胞苷作用MUTZ-8细胞后,凋亡率明显升高,线粒体膜电位明显下降,与阴性对照组相比,差异具有统计学意义(P<0.05)。流式细胞仪及荧光显微镜检测均显示,阿糖胞苷作用后细胞内活性氧(reactive oxygen species, ROS)明显上升。而加入抗氧化剂α-硫辛酸后细胞内ROS表达水平下降。      结论       阿糖胞苷明显抑制了人MDS细胞株MUTZ-8的生长并诱导其凋亡,可能与阿糖胞苷影响了细胞内ROS水平有关。
Abstract:
Objective        To investigate the possible underlying mechanism of apoptosis in MUTZ-8 cells induced by cytarabine.       Methods       The cells were divided into 5 groups, negative control group, 0.1 μmol/L Ara-c group, 1 μmol/L Ara-c group, 10 μmol/L Ara-c group, and 10 μmol/L Ara-c+62.5 μmol/L ALA group. The proliferation of MUTZ-8 cells in above groups was measured by MTT assay. Apoptotic rate was assessed by Annexin V/PI double staining and luminescent dyes-Hoechst33258. Mitochondrial membrane potential of the cells was determined by flow cytometry. Using a ROS probe (2′,7′-dichlo-rofluorescein diacetate, DCFH-DA), the level of reactive oxygen species (ROS) was detected by flow cytometry and fluorescence microscopy.       Results        MTT assay indicated cytarabine resulted in a stronger inhibition in cell viability when compared with the cells of negative control group (P<0.05). Apoptotic rates of the cells in cytarabine groups were significantly increased as compared with negative control group (P<0.05). Mitochondrial membrane potential levels of the cells were decreased in cytarabine groups  as compared with negative control group (P<0.05). ROS levels in all cytarabine groups were up-regulated as compared with negative control group (P<0.05).       Conclusion       Cytarabine effectively inhibits the growth and induces the apoptosis in MUTZ-8 cells, which might be related to the elevation of ROS level.

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更新日期/Last Update: 2012-07-26