[1]常慧君,申涛,董世武,等.purmorphamine促进人牙周膜干细胞应力成骨[J].陆军军医大学学报(原第三军医大学学报),2012,34(09):839-842.
 Chang Huijun,Shen Tao,Dong Shiwu,et al.Purmorphamine promotes osteogenic differentiation in human periodontal ligament stem cells under dynamic tensile[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(09):839-842.
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陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
34卷
期数:
2012年第09期
页码:
839-842
栏目:
论著
出版日期:
2012-05-15

文章信息/Info

Title:
Purmorphamine promotes osteogenic differentiation in human periodontal ligament stem cells under dynamic tensile
作者:
常慧君申涛董世武杨彦春张洁周继祥
第三军医大学:西南医院口腔科,基础医学部人体解剖学教研室;四川省军区门诊部口腔科;中国人民解放军61251部队
Author(s):
Chang Huijun Shen Tao Dong Shiwu Yang Yanchun Zhang Jie Zhou Jixiang
Department of Stomatology, Southwest Hospital, Department of Anatomy, College of Basic Medical Sciences, Third Military Medical University, Chongqing, 400038;Department of Stomatology, Out-patient Department of Sichuan Military Command, Chengdu, Sichuan Province, 610041; No. 61251 Troop, Qinhuangdao, Hebei Province, 066102, China
关键词:
purmorphamine人牙周膜干细胞Hedgehog动态张应力成骨分化
Keywords:
purmorphamine human periodontal ligament stem cells Hedgehog signal pathway dynamic tensile osteoblast differentiation
分类号:
R322.41; R329.2; R345.61
文献标志码:
A
摘要:
目的      研究purmorphamine在动态张应力促人牙周膜干细胞(human periodontal ligament stem cells,PDLSCs)向成骨细胞分化过程中的作用。      方法        分离培养鉴定PDLSCs,在矿化诱导环境中加入purmorphamine,采用Flexcell FX-4000T应力加载系统对细胞加力24 h,以Real-time PCR检测成骨相关指标Runx2、alkaline phosphatase(ALP)以及Hedgehog(Hh)通路的标志物GLI1、Pathed1(PTCH1)、Smoothend(SMO)。      结果         动态张应力作用24 h后,成骨相关指标Runx2、ALP,Hh通路的标志物GLI1、PTCH1、SMO的表达水平明显升高(P<0.05);加入purmorphamine后,成骨相关指标Runx2、ALP,Hh通路的标志物GLI1、PTCH1、SMO的表达水平较加力组均有增强,差异有统计学意义(P<0.05)。      结论        purmorphamine可能通过激活Hh通路,进而增强人PDLSCs应力条件下向成骨细胞分化。
Abstract:
Objective          To determine the effect of purmorphamine on the osteogenic differentiation in human periodontal ligament stem cells (PDLSCs) under dynamic strain.       Methods        PDLSCs were isolated from freshly extracted teeth and identified with flow cytometry for expression of STRO-1 and CD146, and then subcultured into six-well flexible-bottomed Uniflex culture plates. When 80% confluence was achieved, purmorphamine, an activator of the hedgehog (Hh) signaling pathway, was added into the wells in mineralization induction medium at the concentration of 1 μmol/L, and dynamic strains were applied to PDLSCs with the Flexcell FX-4000T Tension Plus System for 24 h. Then the mRNA expression of osteoblastic related indexes of Runx2, ALP and Hh pathway markers GLI1, PTCH1, SMO was detected by real-time PCR.       Results         After 24 h loading, the gene expression of osteogenic markers Runx2 and ALP and Hh pathway markers (GLI1, PTCH1 and SMO) was all increased (P<0.05). And the group with purmorphamine treatment showed much higher expression of these genes than  the control group and the only mechanical loading group (P<0.05).       Conclusion        Purmorphamine promotes osteogenic differentiation in PDLSCs under dynamic tensile, probably through activation of Hh signal pathway.

相似文献/References:

[1]张洁,常慧君,李雨轩,等.Smoothened慢病毒干扰载体的构建及其对人牙周膜干细胞增殖与分化的影响[J].陆军军医大学学报(原第三军医大学学报),2013,35(11):1133.
 Zhang Jie,Chang Huijun,Li Yuxuan,et al.Construction of lentiviral RNAi vectors containing human smoothened gene and its inhibitory effect on proliferation and differentiation in human periodontal ligament stem cells[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(09):1133.

更新日期/Last Update: 2012-05-07