[1]刘红云,王建春.CD200/CD200R在大鼠脓毒症急性肺损伤中的表达[J].第三军医大学学报,2012,34(15):1487-1491.
 Liu Hongyun,Wang Jianchun.Expression profiles of CD200/CD200R in rat septic acute lung injury[J].J Third Mil Med Univ,2012,34(15):1487-1491.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
34卷
期数:
2012年第15期
页码:
1487-1491
栏目:
论著
出版日期:
2012-08-15

文章信息/Info

Title:
Expression profiles of CD200/CD200R in rat septic acute lung injury
作者:
刘红云王建春
第三军医大学新桥医院全军呼吸内科研究所,全军呼吸病研究重点实验室
Author(s):
Liu Hongyun Wang Jianchun
Institute of Respiratory Diseases, Xinqiao Hospital, Third Military Medical University, Chongqing, 400037, China
关键词:
急性肺损伤CD200/CD200R脓毒症
Keywords:
acute lung injury CD200/CD200R sepsis
分类号:
R392.3; R563.02; R631.1
文献标志码:
A
摘要:
目的      研究脓毒症致大鼠急性肺损伤肺组织中CD200/CD200R的表达。      方法        采用盲肠结扎穿孔术复制大鼠脓毒症急性肺损伤(ALI)模型,将36只大鼠随机分为急性肺损伤组和假手术组,每组设8、16、24 h 3个观察时间点,急性肺损伤组为A、B、C组,假手术组与之相对应的时间点分别为D、E、F组,各组大鼠在相应的时间点行动脉血气分析、检测IL-4、肺湿/干质量比测定、肺组织病理学观察,采用RT-PCR法检测肺组织CD200/CD200R mRNA表达、免疫印迹法检测肺组织CD200/CD200R的蛋白表达。      结果        ①CD200/CD200R蛋白及mRNA表达情况:急性肺损伤A、B组较相应对照D、E组表达显著降低(P<0.05),而急性肺损伤C组与相应对照F组比较无显著差异(P>0.05);B组较A组表达显著上升(P<0.05),C组较B组表达显著上升(P<0.05),急性肺损伤组随着病情进展CD200/CD200R的蛋白及mRNA逐渐恢复至假手术对照组水平,各组间差异有统计学意义(P<0.05);②动脉血气分析:A组较相应对照D组无显著差异(P>0.05),B、C组较相应对照E、F组显著降低(P<0.05);急性肺损伤组随着病情进展动脉血氧分压逐渐降低;③IL-4检测:A组较相应对照D组无显著差异(P>0.05),B、C组较相应对照E、F组显著升高(P<0.05),急性肺损伤组随着病情进展IL-4浓度逐渐增加,差异有统计学意义(P<0.05);④肺湿/干质量比:A组较相应对照D组无显著差异(P>0.05),B、C组较相应对照E、F组显著升高(P<0.05),急性肺损伤组随着病情进展肺湿/干质量比逐渐增加,差异有统计学意义(P<0.05);肺组织病理变化:急性肺损伤组随病情进展,病理变化逐渐加重,假手术组病理学变化不明显。      结论        急性肺损伤组大鼠肺组织CD200/CD200R蛋白及mRNA 早期表达降低,至24 h恢复至假手组水平,IL-4呈逐渐升高趋势,参与肺损伤炎症反应,提示CD200/CD200R参与脓毒症ALI。
Abstract:
Objective        To investigate the expression  of CD200/CD200R in septic acute lung injury in rats.       Methods       Thirty-six Wistar rats were randomly divided into the sepsis group and sham-operation control group. Sepsis was induced by cecal ligation and perforation. Rats of the 2 groups were respectively sacrificed in 8, 16, and 24 h respectively after model establishment. Blood samples were collected from the abdominal artery of rats for blood gas analysis. Tissues from lung of rats were embedded for histological analysis, and wet/dry weight. The serum level of IL-4 were determined by ELISA. The expression of CD200/CD200R in the lung tissue at mRNA and protein levels was measured by RT-PCR and Western blotting.       Results      CD200/CD200R expression at mRNA and protein levels was significantly lower in septic acute lung injury group than in control group in 8 and 16 h after injury (P<0.05), but not in 24 h (P>0.05). In septic acute lung injury group, the levels were highest in 24 h, followed by those in 16 h and in 8 h (both P<0.05). The highest levels in 24 h were then decreased gradually to the levels of sham-operation control. Arterial blood gas analysis showed there was no significant in partial pressure of oxygen in 8 h between the 2 groups. The pressures in 16 and 24 h were respectively higher in septic acute lung injury group than in control group (P<0.05). As the disease progressed, the pressure of oxygen was reduced gradually in septic acute lung injury group. There was no significant difference in IL-4 level at 8 h between septic acute lung injury group and control group. IL-4 level was higher in 16 and 24 h in septic acute lung injury group than control group  (P<0.05), which became higher with progress of the disease. There was no significant difference in lung wet/dry weight ratio in 8 h between septic acute lung injury group and control group. ratio was higher in 16 and 24 h in septic acute lung injury group than control group (P<0.05), which was increased with the progression of the disease (P<0.05). The pathological damage of septic acute lung injury group became severe with the disease progression, but no such change was found in the control group.       Conclusion      Expression of CD200/CD200R at mRNA and protein levels is decreased in the lung in rats at early stage of septic acute lung injury, and then restore to the levels of control group. CD200/CD200R is involved in the progression of septic acute lung injury.

参考文献/References:

刘红云, 王建春. CD200/CD200R在大鼠脓毒症急性肺损伤中的表达[J].第三军医大学学报,2012,34(15):1487-1491.

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更新日期/Last Update: 2012-07-26