[1]王绩英,王涛,曾锦荣,等.三氧化二砷联合非诺贝特对人肺癌A549细胞上皮间质转化及E-cadherin/Snail转化因子的影响[J].陆军军医大学学报(原第三军医大学学报),2012,34(14):1406-1410.
 Wang Jiying,Wang Tao,Zeng Jinrong,et al.Effect of combined arsenic trioxide and fenofibrate on epithelial-interstitial transformation and E-cadherin/Snail transformation factor in human pulmonary carcinoma A549 cells[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(14):1406-1410.
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三氧化二砷联合非诺贝特对人肺癌A549细胞上皮间质转化及E-cadherin/Snail转化因子的影响(/HTML )
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陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
34卷
期数:
2012年第14期
页码:
1406-1410
栏目:
论著
出版日期:
2012-07-30

文章信息/Info

Title:
Effect of combined arsenic trioxide and fenofibrate on epithelial-interstitial transformation and E-cadherin/Snail transformation factor in human pulmonary carcinoma A549 cells
作者:
王绩英王涛曾锦荣刘春妮莫碧文陈梅唏陈峰林武洲
桂林医学院附属医院呼吸内科
Author(s):
Wang Jiying Wang Tao Zeng Jinrong Liu Chunni Mo Biwen Chen Meixi Chen Feng Lin Wuzhou
Department of Respiratory Diseases, Affiliated Hospital of Guilin Medical College, Guilin, Guangxi Zhuang Autonomous Region, 541001, China
关键词:
三氧化二砷非诺贝特肺肿瘤上皮-间质转化E-cadherinSnail
Keywords:
arsenic trioxide fenofibrate lung neoplasm epithelial-interstitial transformationE-cadherin Snail
分类号:
R734.2;R966;R979.1
文献标志码:
A
摘要:
目的      观察三氧化二砷和非诺贝特单独及联合运用对人肺癌A549细胞上皮间质转化及相关因子E-cadherin、Snail的影响。      方法      体外培养人肺癌A549细胞,根据处理因素不同分为4组,A组:阴性对照组(只含有DMEM培养液);B组:1 μmol/L三氧化二砷单药处理组;C组:100 μmol/L非诺贝特单药处理组;D组:1 μmol/L三氧化二砷+100 μmol/L非诺贝特联合处理组。干预A549细胞48 h后,分别运用MTT法检测对A549细胞的抑制作用,流式细胞术检测对A549细胞周期的影响,小室侵袭实验检测对A549细胞侵袭能力的影响,半定量RT-PCR检测对E-cadherin、Snail mRNA表达的影响,Western blot检测对E-cadherin、Snail蛋白表达的影响。      结果      与阴性对照组及单药组相比,三氧化二砷联合非诺贝特可以明显抑制A549细胞的活性,其中三氧化二砷组的抑制率为(17.62±0.51)%,非诺贝特组的抑制率为(28.30±0.27)%,而联合组的抑制率为(35.19±0.04)%,差异有统计学意义(P<0.05)。两者联合还可以干扰A549细胞的细胞周期,减弱A549细胞的侵袭能力,3组的侵袭抑制率分别为:三氧化二砷组(50.3±0.15)%,非诺贝特组(56.7±0.57)%,联合组(68.1±0.21)%,差异有统计学意义(P<0.05)。RT-PCR和Western blot检测结果显示,联合组较阴性组及单药组明显上调E-cadherin mRNA及蛋白的表达,下调Snail mRNA及蛋白的表达,差异有统计学意义(P<0.05)。      结论      三氧化二砷联合非诺贝特有明显的增效作用,两者联合可以增加影响人肺癌A549细胞的上皮间质转化的效果,其机制可能与E-cadherin及Snail相关。
Abstract:
Objective      To observe the effect of combined arsenic trioxide and fenofibrate on epithelial-interstitial transformation and E-cadherin/Snail transformation factor in human pulmonary carcinoma A549 cells.       Methods      Human pulmonary carcinoma A549 cells,cultured in vitro,were divided into group A (control group) containing only DMEM culture fluid, group B (1 μmol/L arsenic trioxide treatment group), group C (100 μmol/L fenofibrate treatment group), and group D (1 μmol/L arsenic trioxide plus 100 μmol/L fenofibrate treatment group). After the A549 cells were exposed to arsenic trioxide, fenofibrate, arsenic trioxide, and fenofibrate, respectively, for 48 h in vitro, their inhibitory effect on proliferation,cell cycle,invasive ability of A549 cells,and expression of E-cadherin/Snail mRNA and protein in A549 cells were detected by MTT assay, flow cytometry, RT-PCR and Western blotting, respectively.       Results      The activity of A549 cells was significantly lower in group D than in groups A to C. The inhibition rate of the activity of A549 cells was (17.62±0.51)%, (28.30±0.27)% and (35.19±0.04)%, respectively, in group B, group C and group D (P<0.05). Combined arsenic trioxide and fenofibrate could interference with the cell cycle and reduce the invasive ability of A549 cells with an invasive inhibition rate of (50.3±0.15)%, (56.7±0.57)% and (68.1±0.21)%, respectively, in group B, group C and group D (P<0.05). RT-PCR and Western blotting showed that the expression level of E-cadherin was significantly higher while the expression level of snail was significantly lower in group D than in groups A to C (P<0.05).       Conclusion      Combined arsenic trioxide and fenofibrate exerts a significant effect on epithelial-interstitial transformation of A549 cells, which may be related with the expression of E-cadherin and Snail.

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更新日期/Last Update: 2012-07-12