[1]陈明亮,易龙,金鑫,等.白藜芦醇对TNF-α诱导的血管内皮细胞炎性反应的影响[J].第三军医大学学报,2012,34(13):1255-1258.
 Chen Mingliang,Yi Long,Jin Xin,et al.Effect of resveratrol on TNF-α-induced vascular endothelial inflammation in vitro[J].J Third Mil Med Univ,2012,34(13):1255-1258.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
34卷
期数:
2012年第13期
页码:
1255-1258
栏目:
论著
出版日期:
2012-07-15

文章信息/Info

Title:
Effect of resveratrol on TNF-α-induced vascular endothelial inflammation in vitro
作者:
陈明亮易龙金鑫谢琦周曦陈春烨张婷王丽糜漫天
第三军医大学军事预防医学院营养与食品安全研究中心,重庆市营养与食品安全重点实验室,重庆市医学营养研究中心
Author(s):
Chen Mingliang Yi Long Jin Xin Xie Qi Zhou Xi Chen Chunye Zhang Ting Wang Li Mi Mantian
Research Center for Nutrition and Food Safety, Chongqing Key Laboratory of Nutrition and Food Safety, Chongqing Research Center of Medical Nutrition, College of Preventive Military Medicine, Third Military Medical University, Chongqing, 400038, China
关键词:
动脉粥样硬化白藜芦醇血管内皮细胞炎性反应ICAM-1TNFR1
Keywords:
atherosclerosis resveratrol EA.hy926 inflammation intercellular adhesion molecule-1 tumor necrosis factor receptor-1
分类号:
R151.2; R364.5; R543
文献标志码:
A
摘要:
目的      观察白藜芦醇对TNF-α刺激引起的内皮细胞炎症反应的影响,并围绕TNFR1探索相关作用机制。      方法       不同浓度(0.01、0.1、1、2.5、5、10、20、50、100、200、400 ng/ml)TNF-α处理人血管内皮细胞株EA.hy926,24 h后MTT法和ELISA法分别检测细胞增殖活力和细胞培养液中sICAM-1释放水平;白藜芦醇(浓度为0.1、1、10 μmol/L)预处理 24 h后,再用TNF-α(10 ng/ml)处理24 h,.利用ELISA法检测细胞培养液中sICAM-1释放水平,qRT-PCR法检测ICAM-1 mRNA和TNFR1 mRNA表达水平。      结果       高浓度TNF-α(≥100 ng/ml)刺激EA.hy926细胞后,细胞增殖活力显著下降(P<0.05),当TNF-浓度为200 ng/ml时,与对照组比较细胞增殖活力下降约59.7%;浓度为10 ng/ml的TNF-α刺激内皮细胞后,细胞的ICAM-1及TNFR1 mRNA表达明显上调(P<0.05);白藜芦醇预处理内皮细胞后可明显下调TNFR1 mRNA表达水平(P<0.05),并显著抑制TNF-α诱导的ICAM-1的表达(P<0.05),且抑制作用随着浓度的增加而增强。      结论       白藜芦醇可能通过抑制TNFR1表达,进而抑制TNF-α刺激引起的炎性因子释放,减轻内皮炎性损伤。
Abstract:
Objective       To determine the inhibitory effects of resveratrol on TNF-α induced inflammatory damage in vascular endothelial cell line EA. hy926.       Methods       After the endothelial EA. hy926 cells were treated with TNF-α of different concentrations (0.01, 0.1,1, 2.5, 5, 10, 20, 50, 100, 200, and 400 ng/ml) for 24 h, cell viability and sICAM-1 release in the supernatant were measured by MTT and ELISA assay, respectively. Cells were pretreated with resveratrol of different concentrations (0.1, 1, and 10 μmol/L) for 24 h, and then incubated with TNF-α (10 ng/ml) for another 24 h. sICAM-1 level in the the supernatant was determined by sICAM-1 ELISA kit. The mRNA expression of intercellular adhesion molecule-1 (ICAM-1) and tumor necrosis factor receptor-1 (TNFR1) were measured by qRT-PCR assay.       Results        After treated with TNF-α at high concentration (≥100 ng/ml), cell viability was decreased significantly. Cell viability incubated with 200 ng/ml TNF-α was decreased by about 59.7% compared with control. When treated with a low concentration of TNF-α (10 ng/ml), the mRNA levels of ICAM-1 and TNFR1 were increased markedly (P<0.05). However, resveratrol pretreatment significantly (P<0.01) inhibited the increase of ICAM-1 and TNFR1 mRNA expression induced by TNF-α in a dose-dependent manner.       Conclusion        Resveratrol might inhibit the TNF-α-induced inflammatory cytokine release and alleviate endothelial inflammation by inhibiting TNFR1-mediated signaling pathway.

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更新日期/Last Update: 2012-07-04